Quantitative proteomic assessment of very early cellular signaling events
Research output: Contribution to journal › Journal article › Research › peer-review
Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.
Original language | English |
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Journal | Nature Biotechnology |
Volume | 25 |
Issue number | 5 |
Pages (from-to) | 566-8 |
Number of pages | 2 |
ISSN | 1087-0156 |
DOIs | |
Publication status | Published - 2007 |
Externally published | Yes |
Bibliographical note
Keywords: Fluorescence Recovery After Photobleaching; Metabolic Clearance Rate; Microscopy, Fluorescence; Phosphorylation; Proteome; Receptor, Epidermal Growth Factor; Signal Transduction; Time Factors
ID: 46461904