Quantitative proteomic assessment of very early cellular signaling events
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Quantitative proteomic assessment of very early cellular signaling events. / Dengjel, Joern; Akimov, Vyacheslav; Olsen, Jesper Velgaard; Bunkenborg, Jakob; Mann, Matthias; Blagoev, Blagoy; Andersen, Jens S.
In: Nature Biotechnology, Vol. 25, No. 5, 2007, p. 566-8.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Quantitative proteomic assessment of very early cellular signaling events
AU - Dengjel, Joern
AU - Akimov, Vyacheslav
AU - Olsen, Jesper Velgaard
AU - Bunkenborg, Jakob
AU - Mann, Matthias
AU - Blagoev, Blagoy
AU - Andersen, Jens S
N1 - Keywords: Fluorescence Recovery After Photobleaching; Metabolic Clearance Rate; Microscopy, Fluorescence; Phosphorylation; Proteome; Receptor, Epidermal Growth Factor; Signal Transduction; Time Factors
PY - 2007
Y1 - 2007
N2 - Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.
AB - Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.
U2 - 10.1038/nbt1301
DO - 10.1038/nbt1301
M3 - Journal article
C2 - 17450129
VL - 25
SP - 566
EP - 568
JO - Nature Biotechnology
JF - Nature Biotechnology
SN - 1087-0156
IS - 5
ER -
ID: 46461904