Minimal information for studies of extracellular vesicles 2018 (MISEV2018): a position statement of the International Society for Extracellular Vesicles and update of the MISEV2014 guidelines

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  • Clotilde Théry
  • Kenneth W Witwer
  • Elena Aikawa
  • Maria Jose Alcaraz
  • Johnathon D Anderson
  • Ramaroson Andriantsitohaina
  • Anna Antoniou
  • Tanina Arab
  • Fabienne Archer
  • Georgia K Atkin-Smith
  • D Craig Ayre
  • Jean-Marie Bach
  • Daniel Bachurski
  • Hossein Baharvand
  • Leonora Balaj
  • Shawn Baldacchino
  • Natalie N Bauer
  • Amy A Baxter
  • Mary Bebawy
  • Carla Beckham
  • Apolonija Bedina Zavec
  • Abderrahim Benmoussa
  • Anna C Berardi
  • Paolo Bergese
  • Ewa Bielska
  • Cherie Blenkiron
  • Sylwia Bobis-Wozowicz
  • Eric Boilard
  • Wilfrid Boireau
  • Antonella Bongiovanni
  • Francesc E Borràs
  • Steffi Bosch
  • Chantal M Boulanger
  • Xandra Breakefield
  • Andrew M Breglio
  • Meadhbh Á Brennan
  • David R Brigstock
  • Alain Brisson
  • Marike Ld Broekman
  • Jacqueline F Bromberg
  • Paulina Bryl-Górecka
  • Shilpa Buch
  • Amy H Buck
  • Dylan Burger
  • Sara Busatto
  • Dominik Buschmann
  • Malene Møller Jørgensen
  • Peter Nejsum
  • Jing Xu

The last decade has seen a sharp increase in the number of scientific publications describing physiological and pathological functions of extracellular vesicles (EVs), a collective term covering various subtypes of cell-released, membranous structures, called exosomes, microvesicles, microparticles, ectosomes, oncosomes, apoptotic bodies, and many other names. However, specific issues arise when working with these entities, whose size and amount often make them difficult to obtain as relatively pure preparations, and to characterize properly. The International Society for Extracellular Vesicles (ISEV) proposed Minimal Information for Studies of Extracellular Vesicles ("MISEV") guidelines for the field in 2014. We now update these "MISEV2014" guidelines based on evolution of the collective knowledge in the last four years. An important point to consider is that ascribing a specific function to EVs in general, or to subtypes of EVs, requires reporting of specific information beyond mere description of function in a crude, potentially contaminated, and heterogeneous preparation. For example, claims that exosomes are endowed with exquisite and specific activities remain difficult to support experimentally, given our still limited knowledge of their specific molecular machineries of biogenesis and release, as compared with other biophysically similar EVs. The MISEV2018 guidelines include tables and outlines of suggested protocols and steps to follow to document specific EV-associated functional activities. Finally, a checklist is provided with summaries of key points.

Original languageEnglish
Article number1535750
JournalJournal of Extracellular Vesicles
Issue number1
Pages (from-to)1-47
Publication statusPublished - 2018

Bibliographical note

Position Paper

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