Proteomics and cell signaling in the Choudhary Group

A major interest of the Choudhary Group is to decipher cellular signalling networks by combining the latest genome editing technologies with state-of-the-art quantitative proteomics.

Research focus

The Choudhary Group is interested in obtaining novel understanding of the regulatory mechanisms in cell signaling. In particular, we are interested in unraveling the properties and the functional landscape of posttranslational modifications (PTMs) in signaling networks.

The Group uses the latest genome editing technologies to generate engineered mammalian cell line models and applies the cutting-edge mass spectrometry-based proteomic technologies for unbiased, global, and quantitative analysis of key regulatory PTMs, including lysine acetylation and ubiquitylation. In this endeavour, the Group collaborates extensively with leading researchers from Denmark and around the world.

Main findings

Unraveling lysine deacetylase inhibitor specificities for endogenous acetylation sites
Schölz C, Weinert BT, Wagner SA, Beli P, Miyake Y, Jun QI, Jensen LJ, Streicher W, McCarthy AR, Westwood NJ, Lain S, Cox J, Matthias P, Mann M, Bradner JE, Choudhary C. Nature Biotechnology. 2015 Apr;33(4):415-23. 

The growing landscape of lysine acetylation links metabolism and cell signalling
Choudhary C, Weinert BT, Yoshida Y, Verdin E, Mann M. Nature Reviews Molecular Cell Biology. 2014 Jul 23;15(8):536-50.

Acetyl-Phosphate is a Critical Determinant of Lysine Acetylation in E. coli
Weinert BT, Iesmantavicius V, Wagner SA, Gummesson B, Beli P, Nyström T and Choudhary C.. Molecular Cell. 2013 Jul 25;51(2):265-72.

Staff of the Choudhary Group

Group Leader: Professor Chuna Ram Choudhary

Top row: Takeo Narita, Rajat Gupta, Brian Weinert, Thomas Wild, Lidiya Dimova, Gabriela Zofia Prus
Bottom row: Chuna Ram Choudhary, Magdalena Budzowska, Elina Maskey