Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures. / Cossec, Jack Christophe; Traboulsi, Tatiana; Sart, Sébastien; Loe-Mie, Yann; Guthmann, Manuel; Hendriks, Ivo A.; Theurillat, Ilan; Nielsen, Michael L.; Torres-Padilla, Maria Elena; Baroud, Charles N.; Dejean, Anne.

In: Cell Reports, Vol. 42, No. 4, 112380, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Cossec, JC, Traboulsi, T, Sart, S, Loe-Mie, Y, Guthmann, M, Hendriks, IA, Theurillat, I, Nielsen, ML, Torres-Padilla, ME, Baroud, CN & Dejean, A 2023, 'Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures', Cell Reports, vol. 42, no. 4, 112380. https://doi.org/10.1016/j.celrep.2023.112380

APA

Cossec, J. C., Traboulsi, T., Sart, S., Loe-Mie, Y., Guthmann, M., Hendriks, I. A., Theurillat, I., Nielsen, M. L., Torres-Padilla, M. E., Baroud, C. N., & Dejean, A. (2023). Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures. Cell Reports, 42(4), [112380]. https://doi.org/10.1016/j.celrep.2023.112380

Vancouver

Cossec JC, Traboulsi T, Sart S, Loe-Mie Y, Guthmann M, Hendriks IA et al. Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures. Cell Reports. 2023;42(4). 112380. https://doi.org/10.1016/j.celrep.2023.112380

Author

Cossec, Jack Christophe ; Traboulsi, Tatiana ; Sart, Sébastien ; Loe-Mie, Yann ; Guthmann, Manuel ; Hendriks, Ivo A. ; Theurillat, Ilan ; Nielsen, Michael L. ; Torres-Padilla, Maria Elena ; Baroud, Charles N. ; Dejean, Anne. / Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures. In: Cell Reports. 2023 ; Vol. 42, No. 4.

Bibtex

@article{83db4ffe135a42b0badd1299e5f91499,
title = "Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures",
abstract = "Recent advances in synthetic embryology have opened new avenues for understanding the complex events controlling mammalian peri-implantation development. Here, we show that mouse embryonic stem cells (ESCs) solely exposed to chemical inhibition of SUMOylation generate embryo-like structures comprising anterior neural and trunk-associated regions. HypoSUMOylation-instructed ESCs give rise to spheroids that self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in a droplet microfluidic device form elongated structures that undergo axial organization reminiscent of natural embryo morphogenesis. Single-cell transcriptomics reveals various cellular lineages, including properly positioned anterior neuronal cell types and paraxial mesoderm segmented into somite-like structures. Transient SUMOylation suppression gradually increases DNA methylation genome wide and repressive mark deposition at Nanog. Interestingly, cell-to-cell variations in SUMOylation levels occur during early embryogenesis. Our approach provides a proof of principle for potentially powerful strategies to explore early embryogenesis by targeting chromatin roadblocks of cell fate change.",
keywords = "cell identity, chromatin, CP: Developmental biology, CP: Stem cell research, embryoids, embryonic stem cells, epigenetics, gastruloids, microfluidics, SUMOylation, synthetic embryos",
author = "Cossec, {Jack Christophe} and Tatiana Traboulsi and S{\'e}bastien Sart and Yann Loe-Mie and Manuel Guthmann and Hendriks, {Ivo A.} and Ilan Theurillat and Nielsen, {Michael L.} and Torres-Padilla, {Maria Elena} and Baroud, {Charles N.} and Anne Dejean",
note = "Publisher Copyright: {\textcopyright} 2023 The Author(s)",
year = "2023",
doi = "10.1016/j.celrep.2023.112380",
language = "English",
volume = "42",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Transient suppression of SUMOylation in embryonic stem cells generates embryo-like structures

AU - Cossec, Jack Christophe

AU - Traboulsi, Tatiana

AU - Sart, Sébastien

AU - Loe-Mie, Yann

AU - Guthmann, Manuel

AU - Hendriks, Ivo A.

AU - Theurillat, Ilan

AU - Nielsen, Michael L.

AU - Torres-Padilla, Maria Elena

AU - Baroud, Charles N.

AU - Dejean, Anne

N1 - Publisher Copyright: © 2023 The Author(s)

PY - 2023

Y1 - 2023

N2 - Recent advances in synthetic embryology have opened new avenues for understanding the complex events controlling mammalian peri-implantation development. Here, we show that mouse embryonic stem cells (ESCs) solely exposed to chemical inhibition of SUMOylation generate embryo-like structures comprising anterior neural and trunk-associated regions. HypoSUMOylation-instructed ESCs give rise to spheroids that self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in a droplet microfluidic device form elongated structures that undergo axial organization reminiscent of natural embryo morphogenesis. Single-cell transcriptomics reveals various cellular lineages, including properly positioned anterior neuronal cell types and paraxial mesoderm segmented into somite-like structures. Transient SUMOylation suppression gradually increases DNA methylation genome wide and repressive mark deposition at Nanog. Interestingly, cell-to-cell variations in SUMOylation levels occur during early embryogenesis. Our approach provides a proof of principle for potentially powerful strategies to explore early embryogenesis by targeting chromatin roadblocks of cell fate change.

AB - Recent advances in synthetic embryology have opened new avenues for understanding the complex events controlling mammalian peri-implantation development. Here, we show that mouse embryonic stem cells (ESCs) solely exposed to chemical inhibition of SUMOylation generate embryo-like structures comprising anterior neural and trunk-associated regions. HypoSUMOylation-instructed ESCs give rise to spheroids that self-organize into gastrulating structures containing cell types spatially and functionally related to embryonic and extraembryonic compartments. Alternatively, spheroids cultured in a droplet microfluidic device form elongated structures that undergo axial organization reminiscent of natural embryo morphogenesis. Single-cell transcriptomics reveals various cellular lineages, including properly positioned anterior neuronal cell types and paraxial mesoderm segmented into somite-like structures. Transient SUMOylation suppression gradually increases DNA methylation genome wide and repressive mark deposition at Nanog. Interestingly, cell-to-cell variations in SUMOylation levels occur during early embryogenesis. Our approach provides a proof of principle for potentially powerful strategies to explore early embryogenesis by targeting chromatin roadblocks of cell fate change.

KW - cell identity

KW - chromatin

KW - CP: Developmental biology

KW - CP: Stem cell research

KW - embryoids

KW - embryonic stem cells

KW - epigenetics

KW - gastruloids

KW - microfluidics

KW - SUMOylation

KW - synthetic embryos

U2 - 10.1016/j.celrep.2023.112380

DO - 10.1016/j.celrep.2023.112380

M3 - Journal article

C2 - 37061916

AN - SCOPUS:85152458544

VL - 42

JO - Cell Reports

JF - Cell Reports

SN - 2211-1247

IS - 4

M1 - 112380

ER -

ID: 345374069