Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency

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Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency. / Sukparangsi, Woranop; Morganti, Elena; Lowndes, Molly; Mayeur, Hélène; Weisser, Melanie; Hammachi, Fella; Peradziryi, Hanna; Roske, Fabian; Hölzenspies, Jurriaan; Livigni, Alessandra; Godard, Benoit Gilbert; Sugahara, Fumiaki; Kuratani, Shigeru; Montoya, Guillermo; Frankenberg, Stephen R.; Mazan, Sylvie; Brickman, Joshua M.

In: Nature Communications, Vol. 13, No. 1, 5537, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Sukparangsi, W, Morganti, E, Lowndes, M, Mayeur, H, Weisser, M, Hammachi, F, Peradziryi, H, Roske, F, Hölzenspies, J, Livigni, A, Godard, BG, Sugahara, F, Kuratani, S, Montoya, G, Frankenberg, SR, Mazan, S & Brickman, JM 2022, 'Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency', Nature Communications, vol. 13, no. 1, 5537. https://doi.org/10.1038/s41467-022-32481-z

APA

Sukparangsi, W., Morganti, E., Lowndes, M., Mayeur, H., Weisser, M., Hammachi, F., Peradziryi, H., Roske, F., Hölzenspies, J., Livigni, A., Godard, B. G., Sugahara, F., Kuratani, S., Montoya, G., Frankenberg, S. R., Mazan, S., & Brickman, J. M. (2022). Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency. Nature Communications, 13(1), [5537]. https://doi.org/10.1038/s41467-022-32481-z

Vancouver

Sukparangsi W, Morganti E, Lowndes M, Mayeur H, Weisser M, Hammachi F et al. Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency. Nature Communications. 2022;13(1). 5537. https://doi.org/10.1038/s41467-022-32481-z

Author

Sukparangsi, Woranop ; Morganti, Elena ; Lowndes, Molly ; Mayeur, Hélène ; Weisser, Melanie ; Hammachi, Fella ; Peradziryi, Hanna ; Roske, Fabian ; Hölzenspies, Jurriaan ; Livigni, Alessandra ; Godard, Benoit Gilbert ; Sugahara, Fumiaki ; Kuratani, Shigeru ; Montoya, Guillermo ; Frankenberg, Stephen R. ; Mazan, Sylvie ; Brickman, Joshua M. / Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency. In: Nature Communications. 2022 ; Vol. 13, No. 1.

Bibtex

@article{d0a5174f2ec9404f8383c79d6be46bcb,
title = "Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency",
abstract = "The support of pluripotent cells over time is an essential feature of development. In eutherian embryos, pluripotency is maintained from na{\"i}ve states in peri-implantation to primed pluripotency at gastrulation. To understand how these states emerged, we reconstruct the evolutionary trajectory of the Pou5 gene family, which contains the central pluripotency factor OCT4. By coupling evolutionary sequence analysis with functional studies in mouse embryonic stem cells, we find that the ability of POU5 proteins to support pluripotency originated in the gnathostome lineage, prior to the generation of two paralogues, Pou5f1 and Pou5f3 via gene duplication. In osteichthyans, retaining both genes, the paralogues differ in their support of na{\"i}ve and primed pluripotency. The specialization of these duplicates enables the diversification of function in self-renewal and differentiation. By integrating sequence evolution, cell phenotypes, developmental contexts and structural modelling, we pinpoint OCT4 regions sufficient for na{\"i}ve pluripotency and describe their adaptation over evolutionary time.",
author = "Woranop Sukparangsi and Elena Morganti and Molly Lowndes and H{\'e}l{\`e}ne Mayeur and Melanie Weisser and Fella Hammachi and Hanna Peradziryi and Fabian Roske and Jurriaan H{\"o}lzenspies and Alessandra Livigni and Godard, {Benoit Gilbert} and Fumiaki Sugahara and Shigeru Kuratani and Guillermo Montoya and Frankenberg, {Stephen R.} and Sylvie Mazan and Brickman, {Joshua M.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
doi = "10.1038/s41467-022-32481-z",
language = "English",
volume = "13",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Evolutionary origin of vertebrate OCT4/POU5 functions in supporting pluripotency

AU - Sukparangsi, Woranop

AU - Morganti, Elena

AU - Lowndes, Molly

AU - Mayeur, Hélène

AU - Weisser, Melanie

AU - Hammachi, Fella

AU - Peradziryi, Hanna

AU - Roske, Fabian

AU - Hölzenspies, Jurriaan

AU - Livigni, Alessandra

AU - Godard, Benoit Gilbert

AU - Sugahara, Fumiaki

AU - Kuratani, Shigeru

AU - Montoya, Guillermo

AU - Frankenberg, Stephen R.

AU - Mazan, Sylvie

AU - Brickman, Joshua M.

N1 - Publisher Copyright: © 2022, The Author(s).

PY - 2022

Y1 - 2022

N2 - The support of pluripotent cells over time is an essential feature of development. In eutherian embryos, pluripotency is maintained from naïve states in peri-implantation to primed pluripotency at gastrulation. To understand how these states emerged, we reconstruct the evolutionary trajectory of the Pou5 gene family, which contains the central pluripotency factor OCT4. By coupling evolutionary sequence analysis with functional studies in mouse embryonic stem cells, we find that the ability of POU5 proteins to support pluripotency originated in the gnathostome lineage, prior to the generation of two paralogues, Pou5f1 and Pou5f3 via gene duplication. In osteichthyans, retaining both genes, the paralogues differ in their support of naïve and primed pluripotency. The specialization of these duplicates enables the diversification of function in self-renewal and differentiation. By integrating sequence evolution, cell phenotypes, developmental contexts and structural modelling, we pinpoint OCT4 regions sufficient for naïve pluripotency and describe their adaptation over evolutionary time.

AB - The support of pluripotent cells over time is an essential feature of development. In eutherian embryos, pluripotency is maintained from naïve states in peri-implantation to primed pluripotency at gastrulation. To understand how these states emerged, we reconstruct the evolutionary trajectory of the Pou5 gene family, which contains the central pluripotency factor OCT4. By coupling evolutionary sequence analysis with functional studies in mouse embryonic stem cells, we find that the ability of POU5 proteins to support pluripotency originated in the gnathostome lineage, prior to the generation of two paralogues, Pou5f1 and Pou5f3 via gene duplication. In osteichthyans, retaining both genes, the paralogues differ in their support of naïve and primed pluripotency. The specialization of these duplicates enables the diversification of function in self-renewal and differentiation. By integrating sequence evolution, cell phenotypes, developmental contexts and structural modelling, we pinpoint OCT4 regions sufficient for naïve pluripotency and describe their adaptation over evolutionary time.

U2 - 10.1038/s41467-022-32481-z

DO - 10.1038/s41467-022-32481-z

M3 - Journal article

C2 - 36130934

AN - SCOPUS:85138266299

VL - 13

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

M1 - 5537

ER -

ID: 321475033