NetworKIN: a resource for exploring cellular phosphorylation networks
Research output: Contribution to journal › Journal article › Research › peer-review
Protein kinases control cellular responses by phosphorylating specific substrates. Recent proteome-wide mapping of protein phosphorylation sites by mass spectrometry has discovered thousands of in vivo sites. Systematically assigning all 518 human kinases to all these sites is a challenging problem. The NetworKIN database (http://networkin.info) integrates consensus substrate motifs with context modelling for improved prediction of cellular kinase-substrate relations. Based on the latest human phosphoproteome from the Phospho.ELM and PhosphoSite databases, the resource offers insight into phosphorylation-modulated interaction networks. Here, we describe how NetworKIN can be used for both global and targeted molecular studies. Via the web interface users can query the database of precomputed kinase-substrate relations or obtain predictions on novel phosphoproteins. The database currently contains a predicted phosphorylation network with 20,224 site-specific interactions involving 3978 phosphoproteins and 73 human kinases from 20 families.
Original language | English |
---|---|
Journal | Nucleic Acids Research |
Volume | 36 |
Issue number | Database issue |
Pages (from-to) | D695-9 |
ISSN | 0305-1048 |
DOIs | |
Publication status | Published - 2008 |
Externally published | Yes |
ID: 40740202