Complete Topological Mapping of a Cellular Protein Interactome Reveals Bow-Tie Motifs as Ubiquitous Connectors of Protein Complexes
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Complete Topological Mapping of a Cellular Protein Interactome Reveals Bow-Tie Motifs as Ubiquitous Connectors of Protein Complexes. / Niss, Kristoffer; Gomez-Casado, Cristina; Hjaltelin, Jessica X; Joeris, Thorsten; Agace, William W.; Belling, Kirstine G; Brunak, Søren.
In: Cell Reports, Vol. 31, No. 11, 107763, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Complete Topological Mapping of a Cellular Protein Interactome Reveals Bow-Tie Motifs as Ubiquitous Connectors of Protein Complexes
AU - Niss, Kristoffer
AU - Gomez-Casado, Cristina
AU - Hjaltelin, Jessica X
AU - Joeris, Thorsten
AU - Agace, William W.
AU - Belling, Kirstine G
AU - Brunak, Søren
PY - 2020
Y1 - 2020
N2 - The network topology of a protein interactome is shaped by the function of each protein, making it a resource of functional knowledge in tissues and in single cells. Today, this resource is underused, as complete network topology characterization has proved difficult for large protein interactomes. We apply a matrix visualization and decoding approach to a physical protein interactome of a dendritic cell, thereby characterizing its topology with no prior assumptions of structure. We discover 294 proteins, each forming topological motifs called "bow-ties" that tie together the majority of observed protein complexes. The central proteins of these bow-ties have unique network properties, display multifunctional capabilities, are enriched for essential proteins, and are widely expressed in other cells and tissues. Collectively, the bow-tie motifs are a pervasive and previously unnoted topological trend in cellular interactomes. As such, these results provide fundamental knowledge on how intracellular protein connectivity is organized and operates.
AB - The network topology of a protein interactome is shaped by the function of each protein, making it a resource of functional knowledge in tissues and in single cells. Today, this resource is underused, as complete network topology characterization has proved difficult for large protein interactomes. We apply a matrix visualization and decoding approach to a physical protein interactome of a dendritic cell, thereby characterizing its topology with no prior assumptions of structure. We discover 294 proteins, each forming topological motifs called "bow-ties" that tie together the majority of observed protein complexes. The central proteins of these bow-ties have unique network properties, display multifunctional capabilities, are enriched for essential proteins, and are widely expressed in other cells and tissues. Collectively, the bow-tie motifs are a pervasive and previously unnoted topological trend in cellular interactomes. As such, these results provide fundamental knowledge on how intracellular protein connectivity is organized and operates.
U2 - 10.1016/j.celrep.2020.107763
DO - 10.1016/j.celrep.2020.107763
M3 - Journal article
C2 - 32553166
VL - 31
JO - Cell Reports
JF - Cell Reports
SN - 2211-1247
IS - 11
M1 - 107763
ER -
ID: 243475518