The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition
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The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition. / Zonderland, Gijs; Vanzo, Riccardo; Amitash, Sampath; Martín-Doncel, Elena; Coscia, Fabian; Mund, Andreas; Lerdrup, Mads; Benada, Jan; Boos, Dominik; Toledo, Luis.
In: Molecular Cell, Vol. 82, No. 18, 2022, p. 3350-3365.e7.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - The TRESLIN-MTBP complex couples completion of DNA replication with S/G2 transition
AU - Zonderland, Gijs
AU - Vanzo, Riccardo
AU - Amitash, Sampath
AU - Martín-Doncel, Elena
AU - Coscia, Fabian
AU - Mund, Andreas
AU - Lerdrup, Mads
AU - Benada, Jan
AU - Boos, Dominik
AU - Toledo, Luis
N1 - Publisher Copyright: © 2022 The Authors
PY - 2022
Y1 - 2022
N2 - It has been proposed that ATR kinase senses the completion of DNA replication to initiate the S/G2 transition. In contrast to this model, we show here that the TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently of ATR/CHK1 kinases. TRESLIN-MTBP acts transiently at pre-replication complexes (preRCs) to initiate origin firing and is released after the subsequent recruitment of CDC45. This dynamic behavior of TRESLIN-MTBP implements a monitoring system that checks the activation of replication forks and senses the rate of origin firing to prevent the entry into G2. This system detects the decline in the number of origins of replication that naturally occurs in very late S, which is the signature that cells use to determine the completion of DNA replication and permit the S/G2 transition. Our work introduces TRESLIN-MTBP as a key player in cell-cycle control independent of canonical checkpoints.
AB - It has been proposed that ATR kinase senses the completion of DNA replication to initiate the S/G2 transition. In contrast to this model, we show here that the TRESLIN-MTBP complex prevents a premature entry into G2 from early S-phase independently of ATR/CHK1 kinases. TRESLIN-MTBP acts transiently at pre-replication complexes (preRCs) to initiate origin firing and is released after the subsequent recruitment of CDC45. This dynamic behavior of TRESLIN-MTBP implements a monitoring system that checks the activation of replication forks and senses the rate of origin firing to prevent the entry into G2. This system detects the decline in the number of origins of replication that naturally occurs in very late S, which is the signature that cells use to determine the completion of DNA replication and permit the S/G2 transition. Our work introduces TRESLIN-MTBP as a key player in cell-cycle control independent of canonical checkpoints.
KW - ATR
KW - cell cycle
KW - checkpoint
KW - CHK1
KW - DNA replication
KW - MTBP
KW - origin firing
KW - S/G2 transition
KW - TRESLIN
U2 - 10.1016/j.molcel.2022.08.006
DO - 10.1016/j.molcel.2022.08.006
M3 - Journal article
C2 - 36049481
AN - SCOPUS:85138058778
VL - 82
SP - 3350-3365.e7
JO - Molecular Cell
JF - Molecular Cell
SN - 1097-2765
IS - 18
ER -
ID: 321473826