High accuracy mass spectrometry in large-scale analysis of protein phosphorylation
Research output: Contribution to journal › Journal article › Research › peer-review
With the appearance of a new generation of high-performance hybrid mass spectrometers, high accuracy (sub-parts-per-million) mass spectrometry is becoming increasingly available to a wider scientific community. Here we discuss the advantages of such mass spectrometric instrumentation in the global analysis of protein phosphorylation. We describe a detailed workflow for fractionation and enrichment of phosphopeptides from digests of whole cell/tissue lysates by strong cation exchange and TiO(2) chromatography and their subsequent measurement on an LTQ-Orbitrap mass spectrometer under several acquisition regimes.
Original language | English |
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Journal | Methods in Molecular Biology |
Volume | 492 |
Pages (from-to) | 131-42 |
Number of pages | 11 |
ISSN | 1064-3745 |
DOIs | |
Publication status | Published - 2009 |
Externally published | Yes |
Bibliographical note
Keywords: Amino Acid Sequence; Chromatography, Ion Exchange; Chromatography, Liquid; Hela Cells; Humans; Mass Spectrometry; Molecular Sequence Data; Phosphopeptides; Phosphoproteins; Phosphorylation; Proteomics; Sensitivity and Specificity; Titanium; Trypsin
ID: 14701352