High accuracy mass spectrometry in large-scale analysis of protein phosphorylation

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Standard

High accuracy mass spectrometry in large-scale analysis of protein phosphorylation. / Olsen, Jesper V; Macek, Boris.

In: Methods in Molecular Biology, Vol. 492, 2009, p. 131-42.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Olsen, JV & Macek, B 2009, 'High accuracy mass spectrometry in large-scale analysis of protein phosphorylation', Methods in Molecular Biology, vol. 492, pp. 131-42. https://doi.org/10.1007/978-1-59745-493-3_7

APA

Olsen, J. V., & Macek, B. (2009). High accuracy mass spectrometry in large-scale analysis of protein phosphorylation. Methods in Molecular Biology, 492, 131-42. https://doi.org/10.1007/978-1-59745-493-3_7

Vancouver

Olsen JV, Macek B. High accuracy mass spectrometry in large-scale analysis of protein phosphorylation. Methods in Molecular Biology. 2009;492:131-42. https://doi.org/10.1007/978-1-59745-493-3_7

Author

Olsen, Jesper V ; Macek, Boris. / High accuracy mass spectrometry in large-scale analysis of protein phosphorylation. In: Methods in Molecular Biology. 2009 ; Vol. 492. pp. 131-42.

Bibtex

@article{03977d50aa0d11debc73000ea68e967b,
title = "High accuracy mass spectrometry in large-scale analysis of protein phosphorylation",
abstract = "With the appearance of a new generation of high-performance hybrid mass spectrometers, high accuracy (sub-parts-per-million) mass spectrometry is becoming increasingly available to a wider scientific community. Here we discuss the advantages of such mass spectrometric instrumentation in the global analysis of protein phosphorylation. We describe a detailed workflow for fractionation and enrichment of phosphopeptides from digests of whole cell/tissue lysates by strong cation exchange and TiO(2) chromatography and their subsequent measurement on an LTQ-Orbitrap mass spectrometer under several acquisition regimes.",
author = "Olsen, {Jesper V} and Boris Macek",
note = "Keywords: Amino Acid Sequence; Chromatography, Ion Exchange; Chromatography, Liquid; Hela Cells; Humans; Mass Spectrometry; Molecular Sequence Data; Phosphopeptides; Phosphoproteins; Phosphorylation; Proteomics; Sensitivity and Specificity; Titanium; Trypsin",
year = "2009",
doi = "10.1007/978-1-59745-493-3_7",
language = "English",
volume = "492",
pages = "131--42",
journal = "Methods in Molecular Biology",
issn = "1064-3745",
publisher = "Humana Press",

}

RIS

TY - JOUR

T1 - High accuracy mass spectrometry in large-scale analysis of protein phosphorylation

AU - Olsen, Jesper V

AU - Macek, Boris

N1 - Keywords: Amino Acid Sequence; Chromatography, Ion Exchange; Chromatography, Liquid; Hela Cells; Humans; Mass Spectrometry; Molecular Sequence Data; Phosphopeptides; Phosphoproteins; Phosphorylation; Proteomics; Sensitivity and Specificity; Titanium; Trypsin

PY - 2009

Y1 - 2009

N2 - With the appearance of a new generation of high-performance hybrid mass spectrometers, high accuracy (sub-parts-per-million) mass spectrometry is becoming increasingly available to a wider scientific community. Here we discuss the advantages of such mass spectrometric instrumentation in the global analysis of protein phosphorylation. We describe a detailed workflow for fractionation and enrichment of phosphopeptides from digests of whole cell/tissue lysates by strong cation exchange and TiO(2) chromatography and their subsequent measurement on an LTQ-Orbitrap mass spectrometer under several acquisition regimes.

AB - With the appearance of a new generation of high-performance hybrid mass spectrometers, high accuracy (sub-parts-per-million) mass spectrometry is becoming increasingly available to a wider scientific community. Here we discuss the advantages of such mass spectrometric instrumentation in the global analysis of protein phosphorylation. We describe a detailed workflow for fractionation and enrichment of phosphopeptides from digests of whole cell/tissue lysates by strong cation exchange and TiO(2) chromatography and their subsequent measurement on an LTQ-Orbitrap mass spectrometer under several acquisition regimes.

U2 - 10.1007/978-1-59745-493-3_7

DO - 10.1007/978-1-59745-493-3_7

M3 - Journal article

C2 - 19241030

VL - 492

SP - 131

EP - 142

JO - Methods in Molecular Biology

JF - Methods in Molecular Biology

SN - 1064-3745

ER -

ID: 14701352