Mammalian Micro-C-XL
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Chromosome Conformation Capture (3C) methods are a family of sequencing-based assays to measure the three-dimensional structure of genomes, with Hi-C as the most prominent method in widespread use. The Micro-C-XL protocol is technical variant that improves the resolution and signal-to-noise ratio of the Hi-C protocol and therefore offers enhanced detection of chromatin features such as chromosome loops and fine-grained resolution of topologically associated domains. Here we describe a detailed step-by-step protocol for Micro-C-XL in mammalian cells.
Original language | English |
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Title of host publication | Chromatin |
Editors | Julia Horsfield, Judith Marsman |
Number of pages | 12 |
Publisher | Springer |
Publication date | 2022 |
Pages | 321-332 |
ISBN (Print) | 978-1-0716-2139-4 |
DOIs | |
Publication status | Published - 2022 |
Series | Methods in Molecular Biology |
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Volume | 2458 |
ISSN | 1064-3745 |
Bibliographical note
Publisher Copyright:
© 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Chromatin fiber, Chromosome conformation capture (3C), Chromosome loops, Genome architecture, Hi-C, MNase, Nucleosome, TADs
Research areas
ID: 297037016