Mammalian Micro-C-XL

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Mammalian Micro-C-XL. / Krietenstein, Nils; Rando, Oliver J.

Chromatin. ed. / Julia Horsfield; Judith Marsman. Springer, 2022. p. 321-332 (Methods in Molecular Biology, Vol. 2458).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Krietenstein, N & Rando, OJ 2022, Mammalian Micro-C-XL. in J Horsfield & J Marsman (eds), Chromatin. Springer, Methods in Molecular Biology, vol. 2458, pp. 321-332. https://doi.org/10.1007/978-1-0716-2140-0_17

APA

Krietenstein, N., & Rando, O. J. (2022). Mammalian Micro-C-XL. In J. Horsfield, & J. Marsman (Eds.), Chromatin (pp. 321-332). Springer. Methods in Molecular Biology Vol. 2458 https://doi.org/10.1007/978-1-0716-2140-0_17

Vancouver

Krietenstein N, Rando OJ. Mammalian Micro-C-XL. In Horsfield J, Marsman J, editors, Chromatin. Springer. 2022. p. 321-332. (Methods in Molecular Biology, Vol. 2458). https://doi.org/10.1007/978-1-0716-2140-0_17

Author

Krietenstein, Nils ; Rando, Oliver J. / Mammalian Micro-C-XL. Chromatin. editor / Julia Horsfield ; Judith Marsman. Springer, 2022. pp. 321-332 (Methods in Molecular Biology, Vol. 2458).

Bibtex

@inbook{07d9df18ff5b41caace51fa306641928,
title = "Mammalian Micro-C-XL",
abstract = "Chromosome Conformation Capture (3C) methods are a family of sequencing-based assays to measure the three-dimensional structure of genomes, with Hi-C as the most prominent method in widespread use. The Micro-C-XL protocol is technical variant that improves the resolution and signal-to-noise ratio of the Hi-C protocol and therefore offers enhanced detection of chromatin features such as chromosome loops and fine-grained resolution of topologically associated domains. Here we describe a detailed step-by-step protocol for Micro-C-XL in mammalian cells.",
keywords = "Chromatin fiber, Chromosome conformation capture (3C), Chromosome loops, Genome architecture, Hi-C, MNase, Nucleosome, TADs",
author = "Nils Krietenstein and Rando, {Oliver J.}",
note = "Publisher Copyright: {\textcopyright} 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2140-0_17",
language = "English",
isbn = "978-1-0716-2139-4",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "321--332",
editor = "Horsfield, {Julia } and Judith Marsman",
booktitle = "Chromatin",
address = "Switzerland",

}

RIS

TY - CHAP

T1 - Mammalian Micro-C-XL

AU - Krietenstein, Nils

AU - Rando, Oliver J.

N1 - Publisher Copyright: © 2022, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Chromosome Conformation Capture (3C) methods are a family of sequencing-based assays to measure the three-dimensional structure of genomes, with Hi-C as the most prominent method in widespread use. The Micro-C-XL protocol is technical variant that improves the resolution and signal-to-noise ratio of the Hi-C protocol and therefore offers enhanced detection of chromatin features such as chromosome loops and fine-grained resolution of topologically associated domains. Here we describe a detailed step-by-step protocol for Micro-C-XL in mammalian cells.

AB - Chromosome Conformation Capture (3C) methods are a family of sequencing-based assays to measure the three-dimensional structure of genomes, with Hi-C as the most prominent method in widespread use. The Micro-C-XL protocol is technical variant that improves the resolution and signal-to-noise ratio of the Hi-C protocol and therefore offers enhanced detection of chromatin features such as chromosome loops and fine-grained resolution of topologically associated domains. Here we describe a detailed step-by-step protocol for Micro-C-XL in mammalian cells.

KW - Chromatin fiber

KW - Chromosome conformation capture (3C)

KW - Chromosome loops

KW - Genome architecture

KW - Hi-C

KW - MNase

KW - Nucleosome

KW - TADs

U2 - 10.1007/978-1-0716-2140-0_17

DO - 10.1007/978-1-0716-2140-0_17

M3 - Book chapter

C2 - 35103975

AN - SCOPUS:85123966928

SN - 978-1-0716-2139-4

T3 - Methods in Molecular Biology

SP - 321

EP - 332

BT - Chromatin

A2 - Horsfield, Julia

A2 - Marsman, Judith

PB - Springer

ER -

ID: 297037016