Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Standard

Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. / Nakamura, Kyosuke; Groth, Anja; Alabert, Constance.

Histone Methyltransferases. ed. / R. Margueron; D. Holoch. Humana Press, 2022. p. 407-417 (Methods in Molecular Biology, Vol. 2529).

Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review

Harvard

Nakamura, K, Groth, A & Alabert, C 2022, Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. in R Margueron & D Holoch (eds), Histone Methyltransferases. Humana Press, Methods in Molecular Biology, vol. 2529, pp. 407-417. https://doi.org/10.1007/978-1-0716-2481-4_17

APA

Nakamura, K., Groth, A., & Alabert, C. (2022). Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. In R. Margueron, & D. Holoch (Eds.), Histone Methyltransferases (pp. 407-417). Humana Press. Methods in Molecular Biology Vol. 2529 https://doi.org/10.1007/978-1-0716-2481-4_17

Vancouver

Nakamura K, Groth A, Alabert C. Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. In Margueron R, Holoch D, editors, Histone Methyltransferases. Humana Press. 2022. p. 407-417. (Methods in Molecular Biology, Vol. 2529). https://doi.org/10.1007/978-1-0716-2481-4_17

Author

Nakamura, Kyosuke ; Groth, Anja ; Alabert, Constance. / Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture. Histone Methyltransferases. editor / R. Margueron ; D. Holoch. Humana Press, 2022. pp. 407-417 (Methods in Molecular Biology, Vol. 2529).

Bibtex

@inbook{41990ad516cb44dc8fb949cf6de22a0d,
title = "Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture",
abstract = "Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.",
keywords = "Chromatin/genetics, Histone Code, Histones/genetics, Mass Spectrometry, Protein Processing, Post-Translational",
author = "Kyosuke Nakamura and Anja Groth and Constance Alabert",
note = "{\textcopyright} 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2022",
doi = "10.1007/978-1-0716-2481-4_17",
language = "English",
isbn = "978-1-0716-2480-7",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "407--417",
editor = "R. Margueron and D. Holoch",
booktitle = "Histone Methyltransferases",
address = "United States",

}

RIS

TY - CHAP

T1 - Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

AU - Nakamura, Kyosuke

AU - Groth, Anja

AU - Alabert, Constance

N1 - © 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

PY - 2022

Y1 - 2022

N2 - Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.

AB - Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.

KW - Chromatin/genetics

KW - Histone Code

KW - Histones/genetics

KW - Mass Spectrometry

KW - Protein Processing, Post-Translational

U2 - 10.1007/978-1-0716-2481-4_17

DO - 10.1007/978-1-0716-2481-4_17

M3 - Book chapter

C2 - 35733024

SN - 978-1-0716-2480-7

T3 - Methods in Molecular Biology

SP - 407

EP - 417

BT - Histone Methyltransferases

A2 - Margueron, R.

A2 - Holoch, D.

PB - Humana Press

ER -

ID: 336467761