Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture
Research output: Chapter in Book/Report/Conference proceeding › Book chapter › Research › peer-review
Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.
|Title of host publication||Histone Methyltransferases|
|Editors||R. Margueron, D. Holoch|
|Publication status||Published - 2022|
|Series||Methods in Molecular Biology|
© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
- Chromatin/genetics, Histone Code, Histones/genetics, Mass Spectrometry, Protein Processing, Post-Translational