Investigating Mitotic Inheritance of Histone Posttranslational Modifications by Triple pSILAC Coupled to Nascent Chromatin Capture

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Pulse stable isotope labeling with amino acids in cell culture (pSILAC) coupled to mass spectrometric analysis is a powerful tool to study propagation of histone post-translational modifications (PTMs). We describe the combination of triple pSILAC with pulse-chase labeling of newly replicated DNA by nascent chromatin capture (NCC). This technology tracks newly synthesized and recycled old histones, from deposition to transmission to daughter cells, unveiling principles of histone-based inheritance.

Original languageEnglish
Title of host publicationHistone Methyltransferases
EditorsR. Margueron, D. Holoch
PublisherHumana Press
Publication date2022
ISBN (Print)978-1-0716-2480-7
ISBN (Electronic)978-1-0716-2481-4
Publication statusPublished - 2022
SeriesMethods in Molecular Biology

Bibliographical note

© 2022. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.

    Research areas

  • Chromatin/genetics, Histone Code, Histones/genetics, Mass Spectrometry, Protein Processing, Post-Translational

ID: 336467761