Identification of the transcription factor MAZ as a regulator of erythropoiesis
Research output: Contribution to journal › Journal article › Research › peer-review
Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors (TFs). Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as a TF that binds to the promoter of the erythroid-specific human α-globin gene. Genome-wide mapping in primary human erythroid cells revealed that MAZ also occupies active promoters as well as GATA1-bound enhancer elements of key erythroid genes. Consistent with an important role during erythropoiesis, knockdown of MAZ reduces α-globin expression in K562 cells and impairs differentiation in primary human erythroid cells. Genetic variants in the MAZ locus are associated with changes in clinically important human erythroid traits. Taken together, these findings reveal the zinc-finger TF MAZ to be a previously unrecognized regulator of the erythroid differentiation program.
Original language | English |
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Journal | Blood advances |
Volume | 5 |
Issue number | 15 |
Pages (from-to) | 3002-3015 |
Number of pages | 14 |
ISSN | 2473-9529 |
DOIs | |
Publication status | Published - 10 Aug 2021 |
Externally published | Yes |
Bibliographical note
© 2021 by The American Society of Hematology.
- DNA-Binding Proteins/genetics, Erythroid Cells/metabolism, Erythropoiesis/genetics, Gene Expression Regulation, Humans, K562 Cells, Promoter Regions, Genetic, Transcription Factors/genetics
Research areas
ID: 276701164