Structural molecular biology in the Montoya Group
The Montoya Group works in the interface between biology, physics and chemistry and uses molecular biology, X-ray crystallography and cryo-electron microscopy to dissect the working mechanisms of the macromolecules that constitute the cell’s machinery.
“Our approach is to use advanced methodology to understand basic cellular mechanisms at an atomic level. We investigate the structural details and function of macromolecules involved in cell cycle progression and genome integrity. Deciphering the working mechanisms of these important processes provides the basis for understanding disease.” says Guillermo Montoya
A recent leap in methodological development has overcome some of the barriers that avoided the study of large macromolecular complexes and allows for a degree of accuracy that has not been possible before. The Montoya group is part of this revolution and since 2017 the group has contributed to set up a state-of-the-art cryo-electron microscopy system that have accelerated their research.
Characterization of CRISPR-Cas12a
The CRISPR-Cas Cas12a/Cpf1 endonuclease is emerging as a powerful genome-editing tool. Our analysis of the Cpf1 structure gives important information about its DNA targeting mechanism.
“Structure of the Cpf1 endonuclease R-loop complex after target DNA cleavage” - Stella et al., Nature 2017
"Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity" - Stella et al ., Cell 2018
I-DmoI catalytic mechanisms
We described the first-of-its-kind catalytic mechanism of a homing endonuclease, I-DmoI, as it is cleaving its target DNA.
“Visualizing phosphodiester-bond hydrolysis by an endonuclease”
Molina et al., Nature Structural & Molecular Biology, 2015
XTACC3 and XMAP215 in spindle assembly during mitosis
By dissecting the mechanism by which XTACC3 and XMAP215 interacts, we showed how microtubule elongation and spindle assembly during mitosis is promoted.
“XTACC3-XMAP215 association reveals an asymmetric interaction promoting microtubule elongation”
Mortuza et al., Nature Communications, 2014
Montoya group news
CPR CRISPR spin-out accepted in BioInnovation Institute program
Researchers map mechanisms in the largest CRISPR system
|Anders Fuglsang||Research Assistant||+4535332077|
|Arturo Carabias del Rey||Postdoc||+4535337930|
|Bijoya Paul||PhD Fellow||+4535332341|
|Dea Nikoline Kjæmpe Nielsen||Laboratory Assistant|
|Elisabeth Bragado Nilsson||Research Technician.||+4535330659|
|Ellen Kirstine Lyhne||Laboratory Technician||+4530449698|
|Francisco Javier Tenjo Castano||PhD Fellow||+4535336501|
|Guillermo Montoya||Head of Research||+4535330663|
|Javier Marchena Hurtado||Master Student|
|Magdalena Karolina Masternak||PhD Fellow||+4535332143|
|Melanie Bianca Weisser||Postdoc||+4535336504|
|Mie Gunni Kolmos Pedersen||Laboratory Assistant|
|Nicholas Heelund Sofos||Microscopy specialist||+4535328272|
|Piero Temperini||PhD Student|
|Rasmus Greve Falbe-Hansen||Research Assistant||+4535334380|
|Sigrid Egevang Jensen||Academic Research Staff|
|Simon Erlendsson||Assistant Professor||+4535337150|
|Stefano Stella||Associate Professor||+4535333188|
|Tillmann Hanns Pape||Microscopy specialist||+4535326593|