The Danish Cryo-EM Facility - Copenhagen node

The Danish Cryo-EM Facility offers access to state-of-the-art equipment to collect data in single particle  and tomography. The Facility has two nodes, one at University at Copenhagen and one at Aarhus University. The Copenhagen node is placed at the Core Facility for Integrated Microscopy (CFIM) at the Faculty of Health and Medial Sciences, and coordinated by CPR and Guillermo Montoya.

The Titan Krios G3 is a powerful and flexible high resolution electron microscope for 3D characterization of biological samples. It was funded by a grant from The Novo Nordisk Foundation and was inaugurated in 2017. You can read more about the instrument at University of Copenhagen here.

The facility is open for all users, access is granted based on applications submitted through the Cryo-EM Application Portal Denmark.

Scientific output:

 

Structure of the mini-RNA-guided endonuclease CRISPR-Cas12j3.
Carabias A, Fuglsang A, Temperini P, Pape T, Sofos N, Stella S, Erlendsson S, Montoya G. Nat Commun. 2021 Jul 22;12(1):4476. doi: 10.1038/s41467-021-24707-3.

Structural basis of torque generation in the bi-directional bacterial flagellar motor.
Hu H, Santiveri M, Wadhwa N, Berg HC, Erhardt M, Taylor NMI. Trends Biochem Sci. 2021 Jul 19:S0968-0004(21)00139-0. doi: 10.1016/j.tibs.2021.06.005. Online

Structure and transport mechanism of P5B-ATPases.
Li P, Wang K, Salustros N, Grønberg C, Gourdon P.  Nat Commun. 2021 Jun 25;12(1):3973. doi: 10.1038/s41467-021-24148-y. PMID: 34172751; PMCID: PMC8233418

Structure and Function of Stator Units of the Bacterial Flagellar Motor.
Santiveri M, Roa-Eguiara A, Kühne C, Wadhwa N, Hu H, Berg HC, Erhardt M, Taylor NMI.
Cell. 2020 Oct 1;183(1):244-257.e16. doi: 10.1016/j.cell.2020.08.016. Epub 2020 Sep 14.
PMID: 32931735

Structures of the Cmr-β Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas.
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G.
Mol Cell. 2020 Sep 3;79(5):741-757.e7. doi: 10.1016/j.molcel.2020.07.008. Epub 2020 Jul 29.
PMID: 32730741

Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent.
Bloch M, Santiveri M, Taylor NMI.
Methods Mol Biol. 2020;2127:227-244. doi: 10.1007/978-1-0716-0373-4_16.
PMID: 32112326

Structural basis of CRISPR-Cas Type III prokaryotic defence systems.
Molina R, Sofos N, Montoya G.
Curr Opin Struct Biol. 2020 Jul 23;65:119-129. doi: 10.1016/j.sbi.2020.06.010. Online ahead of print.
PMID: 327
12502 Review

CRISPR-Cas12a: Functional overview and applications.
Paul B, Montoya G.
Biomed J. 2020 Feb;43(1):8-17. doi: 10.1016/j.bj.2019.10.005. Epub 2020 Feb 5.
PMID: 32200959 Free PMC article. Review.

Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas.
Molina R, Stella S, Feng M, Sofos N, Jauniskis V, Pozdnyakova I, López-Méndez B, She Q, Montoya G.
Nat Commun. 2019 Sep 20;10(1):4302. doi: 10.1038/s41467-019-12244-z.
PMID: 31541109 Free PMC article

High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage.
Slaymaker IM, Mesa P, Kellner MJ, Kannan S, Brignole E, Koob J, Feliciano PR, Stella S, Abudayyeh OO, Gootenberg JS, Strecker J, Montoya G, Zhang F.
Cell Rep. 2019 Mar 26;26(13):3741-3751.e5. doi: 10.1016/j.celrep.2019.02.094.
PMID: 30917325 Free PMC article.

Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Stella S, Mesa P, Thomsen J, Paul B, Alcón P, Jensen SB, Saligram B, Moses ME, Hatzakis NS, Montoya G.
Cell. 2018 Dec 13;175(7):1856-1871.e21. doi: 10.1016/j.cell.2018.10.045. Epub 2018 Nov 29.
PMID: 30503205

 

 

7OP8
7OP5
7OP1
7OP3
7ODF 
6QZT 
6GTC 
6GTD  
6GTE  
6GTG, 
6GTF
6S6B 
6SIC 
6S8B 
6S91 
6S8E  
6SHB 
6SH8
6YKM
6YKP
6YKR 

 

EMD-13014
EMD-13013
EMD-13011
EMD-13012
EMD-12827
EMD-10828
EMD-10829
EMD-10830
EMD-10831
EMD-10832

EMD-4691
EMD-0061
EMD-0062
EMD-0063
EMD-0065
EMD-0064 
EMD-10102 
EMD-10209 
EMD 10117
EMD: 10126 
EMD: 10119 
EMD: 10197
EMD: 10196 

 

 

 

 

Guillermo Montoya, Tillmann Pape