The Danish Cryo-EM Facility - Copenhagen node
The Danish Cryo-EM Facility offers access to state-of-the-art equipment to collect data in single particle and tomography. The Facility has two nodes, one at University at Copenhagen and one at Aarhus University. The Copenhagen node is placed at the Core Facility for Integrated Microscopy (CFIM) at the Faculty of Health and Medial Sciences, and coordinated by CPR and Guillermo Montoya.
The Titan Krios G3 is a powerful and flexible high resolution electron microscope for 3D characterization of biological samples. It was funded by a grant from The Novo Nordisk Foundation and was inaugurated in 2017. You can read more about the instrument at University of Copenhagen here.
The facility is open for all users, access is granted based on applications submitted through the Cryo-EM Application Portal Denmark.
Structure and Function of Stator Units of the Bacterial Flagellar Motor.
Santiveri M, Roa-Eguiara A, Kühne C, Wadhwa N, Hu H, Berg HC, Erhardt M, Taylor NMI.
Cell. 2020 Oct 1;183(1):244-257.e16. doi: 10.1016/j.cell.2020.08.016. Epub 2020 Sep 14.
Structures of the Cmr-β Complex Reveal the Regulation of the Immunity Mechanism of Type III-B CRISPR-Cas.
Sofos N, Feng M, Stella S, Pape T, Fuglsang A, Lin J, Huang Q, Li Y, She Q, Montoya G.
Mol Cell. 2020 Sep 3;79(5):741-757.e7. doi: 10.1016/j.molcel.2020.07.008. Epub 2020 Jul 29.
Membrane Protein Cryo-EM: Cryo-Grid Optimization and Data Collection with Protein in Detergent.
Bloch M, Santiveri M, Taylor NMI.
Methods Mol Biol. 2020;2127:227-244. doi: 10.1007/978-1-0716-0373-4_16.
Structural basis of CRISPR-Cas Type III prokaryotic defence systems.
Molina R, Sofos N, Montoya G.
Curr Opin Struct Biol. 2020 Jul 23;65:119-129. doi: 10.1016/j.sbi.2020.06.010. Online ahead of print.
PMID: 32712502 Review
CRISPR-Cas12a: Functional overview and applications.
Paul B, Montoya G.
Biomed J. 2020 Feb;43(1):8-17. doi: 10.1016/j.bj.2019.10.005. Epub 2020 Feb 5.
PMID: 32200959 Free PMC article. Review.
Structure of Csx1-cOA4 complex reveals the basis of RNA decay in Type III-B CRISPR-Cas.
Molina R, Stella S, Feng M, Sofos N, Jauniskis V, Pozdnyakova I, López-Méndez B, She Q, Montoya G.
Nat Commun. 2019 Sep 20;10(1):4302. doi: 10.1038/s41467-019-12244-z.
PMID: 31541109 Free PMC article
High-Resolution Structure of Cas13b and Biochemical Characterization of RNA Targeting and Cleavage.
Slaymaker IM, Mesa P, Kellner MJ, Kannan S, Brignole E, Koob J, Feliciano PR, Stella S, Abudayyeh OO, Gootenberg JS, Strecker J, Montoya G, Zhang F.
Cell Rep. 2019 Mar 26;26(13):3741-3751.e5. doi: 10.1016/j.celrep.2019.02.094.
PMID: 30917325 Free PMC article.
Conformational Activation Promotes CRISPR-Cas12a Catalysis and Resetting of the Endonuclease Activity.
Stella S, Mesa P, Thomsen J, Paul B, Alcón P, Jensen SB, Saligram B, Moses ME, Hatzakis NS, Montoya G.
Cell. 2018 Dec 13;175(7):1856-1871.e21. doi: 10.1016/j.cell.2018.10.045. Epub 2018 Nov 29.
All publications that include data generated at the Cryo-EM facility must include the following statement: Data collection was performed at the Danish Cryo-EM Facility at CFIM, University of Copenhagen, supported by grant id NNF0024386