Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit

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Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit. / Hu, Haidai; Popp, Philipp F.; Santiveri, Mònica; Roa-Eguiara, Aritz; Yan, Yumeng; Martin, Freddie J.O.; Liu, Zheyi; Wadhwa, Navish; Wang, Yong; Erhardt, Marc; Taylor, Nicholas M.I.

In: Nature Communications, Vol. 14, 4411, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hu, H, Popp, PF, Santiveri, M, Roa-Eguiara, A, Yan, Y, Martin, FJO, Liu, Z, Wadhwa, N, Wang, Y, Erhardt, M & Taylor, NMI 2023, 'Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit', Nature Communications, vol. 14, 4411. https://doi.org/10.1038/s41467-023-39899-z

APA

Hu, H., Popp, P. F., Santiveri, M., Roa-Eguiara, A., Yan, Y., Martin, F. J. O., Liu, Z., Wadhwa, N., Wang, Y., Erhardt, M., & Taylor, N. M. I. (2023). Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit. Nature Communications, 14, [4411]. https://doi.org/10.1038/s41467-023-39899-z

Vancouver

Hu H, Popp PF, Santiveri M, Roa-Eguiara A, Yan Y, Martin FJO et al. Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit. Nature Communications. 2023;14. 4411. https://doi.org/10.1038/s41467-023-39899-z

Author

Hu, Haidai ; Popp, Philipp F. ; Santiveri, Mònica ; Roa-Eguiara, Aritz ; Yan, Yumeng ; Martin, Freddie J.O. ; Liu, Zheyi ; Wadhwa, Navish ; Wang, Yong ; Erhardt, Marc ; Taylor, Nicholas M.I. / Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit. In: Nature Communications. 2023 ; Vol. 14.

Bibtex

@article{cbc48b8b8be34147853142ef9c8aa2e8,
title = "Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit",
abstract = "Bacteria swim using a flagellar motor that is powered by stator units. Vibrio spp. are highly motile bacteria responsible for various human diseases, the polar flagella of which are exclusively driven by sodium-dependent stator units (PomAB). However, how ion selectivity is attained, how ion transport triggers the directional rotation of the stator unit, and how the stator unit is incorporated into the flagellar rotor remained largely unclear. Here, we have determined by cryo-electron microscopy the structure of Vibrio PomAB. The electrostatic potential map uncovers sodium binding sites, which together with functional experiments and molecular dynamics simulations, reveal a mechanism for ion translocation and selectivity. Bulky hydrophobic residues from PomA prime PomA for clockwise rotation. We propose that a dynamic helical motif in PomA regulates the distance between PomA subunit cytoplasmic domains, stator unit activation, and torque transmission. Together, our study provides mechanistic insights for understanding ion selectivity and rotor incorporation of the stator unit of the bacterial flagellum.",
author = "Haidai Hu and Popp, {Philipp F.} and M{\`o}nica Santiveri and Aritz Roa-Eguiara and Yumeng Yan and Martin, {Freddie J.O.} and Zheyi Liu and Navish Wadhwa and Yong Wang and Marc Erhardt and Taylor, {Nicholas M.I.}",
note = "Publisher Copyright: {\textcopyright} 2023, The Author(s).",
year = "2023",
doi = "10.1038/s41467-023-39899-z",
language = "English",
volume = "14",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Ion selectivity and rotor coupling of the Vibrio flagellar sodium-driven stator unit

AU - Hu, Haidai

AU - Popp, Philipp F.

AU - Santiveri, Mònica

AU - Roa-Eguiara, Aritz

AU - Yan, Yumeng

AU - Martin, Freddie J.O.

AU - Liu, Zheyi

AU - Wadhwa, Navish

AU - Wang, Yong

AU - Erhardt, Marc

AU - Taylor, Nicholas M.I.

N1 - Publisher Copyright: © 2023, The Author(s).

PY - 2023

Y1 - 2023

N2 - Bacteria swim using a flagellar motor that is powered by stator units. Vibrio spp. are highly motile bacteria responsible for various human diseases, the polar flagella of which are exclusively driven by sodium-dependent stator units (PomAB). However, how ion selectivity is attained, how ion transport triggers the directional rotation of the stator unit, and how the stator unit is incorporated into the flagellar rotor remained largely unclear. Here, we have determined by cryo-electron microscopy the structure of Vibrio PomAB. The electrostatic potential map uncovers sodium binding sites, which together with functional experiments and molecular dynamics simulations, reveal a mechanism for ion translocation and selectivity. Bulky hydrophobic residues from PomA prime PomA for clockwise rotation. We propose that a dynamic helical motif in PomA regulates the distance between PomA subunit cytoplasmic domains, stator unit activation, and torque transmission. Together, our study provides mechanistic insights for understanding ion selectivity and rotor incorporation of the stator unit of the bacterial flagellum.

AB - Bacteria swim using a flagellar motor that is powered by stator units. Vibrio spp. are highly motile bacteria responsible for various human diseases, the polar flagella of which are exclusively driven by sodium-dependent stator units (PomAB). However, how ion selectivity is attained, how ion transport triggers the directional rotation of the stator unit, and how the stator unit is incorporated into the flagellar rotor remained largely unclear. Here, we have determined by cryo-electron microscopy the structure of Vibrio PomAB. The electrostatic potential map uncovers sodium binding sites, which together with functional experiments and molecular dynamics simulations, reveal a mechanism for ion translocation and selectivity. Bulky hydrophobic residues from PomA prime PomA for clockwise rotation. We propose that a dynamic helical motif in PomA regulates the distance between PomA subunit cytoplasmic domains, stator unit activation, and torque transmission. Together, our study provides mechanistic insights for understanding ion selectivity and rotor incorporation of the stator unit of the bacterial flagellum.

U2 - 10.1038/s41467-023-39899-z

DO - 10.1038/s41467-023-39899-z

M3 - Journal article

C2 - 37500658

AN - SCOPUS:85165929254

VL - 14

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 4411

ER -

ID: 361848522