Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein. / Schimmel, Joost; Eifler, Karolin; Sigurdsson, Jón Otti; Cuijpers, Sabine A G; Hendriks, Ivo A; Verlaan-de Vries, Matty; Kelstrup, Christian D; Francavilla, Chiara; Medema, René H; Olsen, Jesper Velgaard; Vertegaal, Alfred C O.

In: Molecular Cell, Vol. 53, No. 6, 20.03.2014, p. 1053-1066.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Schimmel, J, Eifler, K, Sigurdsson, JO, Cuijpers, SAG, Hendriks, IA, Verlaan-de Vries, M, Kelstrup, CD, Francavilla, C, Medema, RH, Olsen, JV & Vertegaal, ACO 2014, 'Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein', Molecular Cell, vol. 53, no. 6, pp. 1053-1066. https://doi.org/10.1016/j.molcel.2014.02.001

APA

Schimmel, J., Eifler, K., Sigurdsson, J. O., Cuijpers, S. A. G., Hendriks, I. A., Verlaan-de Vries, M., Kelstrup, C. D., Francavilla, C., Medema, R. H., Olsen, J. V., & Vertegaal, A. C. O. (2014). Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein. Molecular Cell, 53(6), 1053-1066. https://doi.org/10.1016/j.molcel.2014.02.001

Vancouver

Schimmel J, Eifler K, Sigurdsson JO, Cuijpers SAG, Hendriks IA, Verlaan-de Vries M et al. Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein. Molecular Cell. 2014 Mar 20;53(6):1053-1066. https://doi.org/10.1016/j.molcel.2014.02.001

Author

Schimmel, Joost ; Eifler, Karolin ; Sigurdsson, Jón Otti ; Cuijpers, Sabine A G ; Hendriks, Ivo A ; Verlaan-de Vries, Matty ; Kelstrup, Christian D ; Francavilla, Chiara ; Medema, René H ; Olsen, Jesper Velgaard ; Vertegaal, Alfred C O. / Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein. In: Molecular Cell. 2014 ; Vol. 53, No. 6. pp. 1053-1066.

Bibtex

@article{04f95086598142c8ac2cd14e476ae381,
title = "Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein",
abstract = "Loss of small ubiquitin-like modification (SUMOylation) in mice causes genomic instability due to the missegregation of chromosomes. Currently, little is known about the identity of relevant SUMO target proteins that are involved in this process and about global SUMOylation dynamics during cell-cycle progression. We performed a large-scale quantitative proteomics screen to address this and identified 593 proteins to be SUMO-2 modified, including the Forkhead box transcription factor M1 (FoxM1), a key regulator of cell-cycle progression and chromosome segregation. SUMOylation of FoxM1 peaks during G2 and M phase, when FoxM1 transcriptional activity is required. We found that a SUMOylation-deficient FoxM1 mutant was less active compared to wild-type FoxM1, implying that SUMOylation of the protein enhances its transcriptional activity. Mechanistically, SUMOylation blocks the dimerization of FoxM1, thereby relieving FoxM1 autorepression. Cells deficient for FoxM1 SUMOylation showed increased levels of polyploidy. Our findings contribute to understanding the role of SUMOylation during cell-cycle progression.",
author = "Joost Schimmel and Karolin Eifler and Sigurdsson, {J{\'o}n Otti} and Cuijpers, {Sabine A G} and Hendriks, {Ivo A} and {Verlaan-de Vries}, Matty and Kelstrup, {Christian D} and Chiara Francavilla and Medema, {Ren{\'e} H} and Olsen, {Jesper Velgaard} and Vertegaal, {Alfred C O}",
note = "Copyright {\textcopyright} 2014 Elsevier Inc. All rights reserved.",
year = "2014",
month = mar,
day = "20",
doi = "10.1016/j.molcel.2014.02.001",
language = "English",
volume = "53",
pages = "1053--1066",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - Uncovering SUMOylation Dynamics during Cell-Cycle Progression Reveals FoxM1 as a Key Mitotic SUMO Target Protein

AU - Schimmel, Joost

AU - Eifler, Karolin

AU - Sigurdsson, Jón Otti

AU - Cuijpers, Sabine A G

AU - Hendriks, Ivo A

AU - Verlaan-de Vries, Matty

AU - Kelstrup, Christian D

AU - Francavilla, Chiara

AU - Medema, René H

AU - Olsen, Jesper Velgaard

AU - Vertegaal, Alfred C O

N1 - Copyright © 2014 Elsevier Inc. All rights reserved.

PY - 2014/3/20

Y1 - 2014/3/20

N2 - Loss of small ubiquitin-like modification (SUMOylation) in mice causes genomic instability due to the missegregation of chromosomes. Currently, little is known about the identity of relevant SUMO target proteins that are involved in this process and about global SUMOylation dynamics during cell-cycle progression. We performed a large-scale quantitative proteomics screen to address this and identified 593 proteins to be SUMO-2 modified, including the Forkhead box transcription factor M1 (FoxM1), a key regulator of cell-cycle progression and chromosome segregation. SUMOylation of FoxM1 peaks during G2 and M phase, when FoxM1 transcriptional activity is required. We found that a SUMOylation-deficient FoxM1 mutant was less active compared to wild-type FoxM1, implying that SUMOylation of the protein enhances its transcriptional activity. Mechanistically, SUMOylation blocks the dimerization of FoxM1, thereby relieving FoxM1 autorepression. Cells deficient for FoxM1 SUMOylation showed increased levels of polyploidy. Our findings contribute to understanding the role of SUMOylation during cell-cycle progression.

AB - Loss of small ubiquitin-like modification (SUMOylation) in mice causes genomic instability due to the missegregation of chromosomes. Currently, little is known about the identity of relevant SUMO target proteins that are involved in this process and about global SUMOylation dynamics during cell-cycle progression. We performed a large-scale quantitative proteomics screen to address this and identified 593 proteins to be SUMO-2 modified, including the Forkhead box transcription factor M1 (FoxM1), a key regulator of cell-cycle progression and chromosome segregation. SUMOylation of FoxM1 peaks during G2 and M phase, when FoxM1 transcriptional activity is required. We found that a SUMOylation-deficient FoxM1 mutant was less active compared to wild-type FoxM1, implying that SUMOylation of the protein enhances its transcriptional activity. Mechanistically, SUMOylation blocks the dimerization of FoxM1, thereby relieving FoxM1 autorepression. Cells deficient for FoxM1 SUMOylation showed increased levels of polyploidy. Our findings contribute to understanding the role of SUMOylation during cell-cycle progression.

U2 - 10.1016/j.molcel.2014.02.001

DO - 10.1016/j.molcel.2014.02.001

M3 - Journal article

C2 - 24582501

VL - 53

SP - 1053

EP - 1066

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 6

ER -

ID: 102260430