Recent findings and technological advances in phosphoproteomics for cells and tissues
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Recent findings and technological advances in phosphoproteomics for cells and tissues. / von Stechow, Louise; Francavilla, Chiara; Olsen, Jesper V.
In: Expert Review of Proteomics, Vol. 12, No. 5, 2015, p. 469-87.Research output: Contribution to journal › Review › Research › peer-review
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TY - JOUR
T1 - Recent findings and technological advances in phosphoproteomics for cells and tissues
AU - von Stechow, Louise
AU - Francavilla, Chiara
AU - Olsen, Jesper V
PY - 2015
Y1 - 2015
N2 - Site-specific phosphorylation is a fast and reversible covalent post-translational modification that is tightly regulated in cells. The cellular machinery of enzymes that write, erase and read these modifications (kinases, phosphatases and phospho-binding proteins) is frequently deregulated in different diseases, including cancer. Large-scale studies of phosphoproteins - termed phosphoproteomics - strongly rely on the use of high-performance mass spectrometric instrumentation. This powerful technology has been applied to study a great number of phosphorylation-based phenotypes. Nevertheless, many technical and biological challenges have to be overcome to identify biologically relevant phosphorylation sites in cells and tissues. This review describes different technological strategies to identify and quantify phosphorylation sites with high accuracy, without significant loss of analysis speed and reproducibility in tissues and cells. Moreover, computational tools for analysis, integration and biological interpretation of phosphorylation events are discussed.
AB - Site-specific phosphorylation is a fast and reversible covalent post-translational modification that is tightly regulated in cells. The cellular machinery of enzymes that write, erase and read these modifications (kinases, phosphatases and phospho-binding proteins) is frequently deregulated in different diseases, including cancer. Large-scale studies of phosphoproteins - termed phosphoproteomics - strongly rely on the use of high-performance mass spectrometric instrumentation. This powerful technology has been applied to study a great number of phosphorylation-based phenotypes. Nevertheless, many technical and biological challenges have to be overcome to identify biologically relevant phosphorylation sites in cells and tissues. This review describes different technological strategies to identify and quantify phosphorylation sites with high accuracy, without significant loss of analysis speed and reproducibility in tissues and cells. Moreover, computational tools for analysis, integration and biological interpretation of phosphorylation events are discussed.
U2 - 10.1586/14789450.2015.1078730
DO - 10.1586/14789450.2015.1078730
M3 - Review
C2 - 26400465
VL - 12
SP - 469
EP - 487
JO - Expert Review of Proteomics
JF - Expert Review of Proteomics
SN - 1478-9450
IS - 5
ER -
ID: 152960126