Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells. / Niss, Kristoffer; Jakobsson, Magnus E.; Westergaard, David; Belling, Kirstine G.; Olsen, Jesper V.; Brunak, Søren.

In: Molecular and Cellular Endocrinology, Vol. 517, 110923, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Niss, K, Jakobsson, ME, Westergaard, D, Belling, KG, Olsen, JV & Brunak, S 2020, 'Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells', Molecular and Cellular Endocrinology, vol. 517, 110923. https://doi.org/10.1016/j.mce.2020.110923

APA

Niss, K., Jakobsson, M. E., Westergaard, D., Belling, K. G., Olsen, J. V., & Brunak, S. (2020). Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells. Molecular and Cellular Endocrinology, 517, [110923]. https://doi.org/10.1016/j.mce.2020.110923

Vancouver

Niss K, Jakobsson ME, Westergaard D, Belling KG, Olsen JV, Brunak S. Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells. Molecular and Cellular Endocrinology. 2020;517. 110923. https://doi.org/10.1016/j.mce.2020.110923

Author

Niss, Kristoffer ; Jakobsson, Magnus E. ; Westergaard, David ; Belling, Kirstine G. ; Olsen, Jesper V. ; Brunak, Søren. / Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells. In: Molecular and Cellular Endocrinology. 2020 ; Vol. 517.

Bibtex

@article{5733016dec84408980e032d968612de0,
title = "Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells",
abstract = "Activated transcription factor (TF) farnesoid X receptor (FXR) represses glucagon-like peptide-1 (GLP-1) secretion in enteroendocrine L cells. This, in turn, reduces insulin secretion, which is triggered when β cells bind GLP-1. Preventing FXR activation could boost GLP-1 production and insulin secretion. Yet, FXR's broader role in L cell biology still lacks understanding. Here, we show that FXR is a multifaceted TF in L cells using proteomics and gene expression data generated on GLUTag L cells. Most striking, 252 proteins regulated upon glucose stimulation have their abundances neutralized upon FXR activation. Mitochondrial repression or glucose import block are likely mechanisms of this. Further, FXR physically targets bile acid metabolism proteins, growth factors and other TFs, regulates ChREBP, while extensive text-mining found 30 FXR-regulated proteins to be well-known in L cell biology. Taken together, this outlines FXR as a powerful TF, where GLP-1 secretion block is just one of many downstream effects.",
author = "Kristoffer Niss and Jakobsson, {Magnus E.} and David Westergaard and Belling, {Kirstine G.} and Olsen, {Jesper V.} and S{\o}ren Brunak",
year = "2020",
doi = "10.1016/j.mce.2020.110923",
language = "English",
volume = "517",
journal = "Molecular and Cellular Endocrinology",
issn = "0303-7207",
publisher = "Elsevier Ireland Ltd",

}

RIS

TY - JOUR

T1 - Effects of active farnesoid X receptor on GLUTag enteroendocrine L cells

AU - Niss, Kristoffer

AU - Jakobsson, Magnus E.

AU - Westergaard, David

AU - Belling, Kirstine G.

AU - Olsen, Jesper V.

AU - Brunak, Søren

PY - 2020

Y1 - 2020

N2 - Activated transcription factor (TF) farnesoid X receptor (FXR) represses glucagon-like peptide-1 (GLP-1) secretion in enteroendocrine L cells. This, in turn, reduces insulin secretion, which is triggered when β cells bind GLP-1. Preventing FXR activation could boost GLP-1 production and insulin secretion. Yet, FXR's broader role in L cell biology still lacks understanding. Here, we show that FXR is a multifaceted TF in L cells using proteomics and gene expression data generated on GLUTag L cells. Most striking, 252 proteins regulated upon glucose stimulation have their abundances neutralized upon FXR activation. Mitochondrial repression or glucose import block are likely mechanisms of this. Further, FXR physically targets bile acid metabolism proteins, growth factors and other TFs, regulates ChREBP, while extensive text-mining found 30 FXR-regulated proteins to be well-known in L cell biology. Taken together, this outlines FXR as a powerful TF, where GLP-1 secretion block is just one of many downstream effects.

AB - Activated transcription factor (TF) farnesoid X receptor (FXR) represses glucagon-like peptide-1 (GLP-1) secretion in enteroendocrine L cells. This, in turn, reduces insulin secretion, which is triggered when β cells bind GLP-1. Preventing FXR activation could boost GLP-1 production and insulin secretion. Yet, FXR's broader role in L cell biology still lacks understanding. Here, we show that FXR is a multifaceted TF in L cells using proteomics and gene expression data generated on GLUTag L cells. Most striking, 252 proteins regulated upon glucose stimulation have their abundances neutralized upon FXR activation. Mitochondrial repression or glucose import block are likely mechanisms of this. Further, FXR physically targets bile acid metabolism proteins, growth factors and other TFs, regulates ChREBP, while extensive text-mining found 30 FXR-regulated proteins to be well-known in L cell biology. Taken together, this outlines FXR as a powerful TF, where GLP-1 secretion block is just one of many downstream effects.

U2 - 10.1016/j.mce.2020.110923

DO - 10.1016/j.mce.2020.110923

M3 - Journal article

C2 - 32702472

VL - 517

JO - Molecular and Cellular Endocrinology

JF - Molecular and Cellular Endocrinology

SN - 0303-7207

M1 - 110923

ER -

ID: 245620719