Ctk1 function is necessary for full translation initiation activity in Saccharomyces cerevisiae
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Ctk1 function is necessary for full translation initiation activity in Saccharomyces cerevisiae. / Coordes, Britta; Brünger, Katharina M; Burger, Kaspar; Soufi, Boumediene; Horenk, Juliane; Eick, Dirk; Olsen, Jesper V; Sträßer, Katja.
In: Eukaryotic Cell (Online Edition), Vol. 14, No. 1, 01.2015, p. 86.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Ctk1 function is necessary for full translation initiation activity in Saccharomyces cerevisiae
AU - Coordes, Britta
AU - Brünger, Katharina M
AU - Burger, Kaspar
AU - Soufi, Boumediene
AU - Horenk, Juliane
AU - Eick, Dirk
AU - Olsen, Jesper V
AU - Sträßer, Katja
N1 - Copyright © 2014, American Society for Microbiology. All Rights Reserved.
PY - 2015/1
Y1 - 2015/1
N2 - Translation is a fundamental and highly regulated cellular process. Previously, we reported that the kinase and transcription elongation factor Ctk1 increases fidelity during translation elongation in Saccharomyces cerevisiae. Here, we show that loss of Ctk1 function also affects the initiation step of translation. Translation active extracts from Ctk1 depleted cells show impaired translation activity of capped, but not CrPV IRES containing mRNA reporters. Furthermore, the formation of 80S initiation complexes is decreased, which is probably due to reduced subunit joining. In addition, we determined the changes in the phosphorylation pattern of a ribosome enriched fraction after depletion of Ctk1. Thus, we provide a catalogue of phosphoproteomic changes dependent on Ctk1. Taken together, our data suggest a stimulatory function of Ctk1 in 80S formation during translation initiation.
AB - Translation is a fundamental and highly regulated cellular process. Previously, we reported that the kinase and transcription elongation factor Ctk1 increases fidelity during translation elongation in Saccharomyces cerevisiae. Here, we show that loss of Ctk1 function also affects the initiation step of translation. Translation active extracts from Ctk1 depleted cells show impaired translation activity of capped, but not CrPV IRES containing mRNA reporters. Furthermore, the formation of 80S initiation complexes is decreased, which is probably due to reduced subunit joining. In addition, we determined the changes in the phosphorylation pattern of a ribosome enriched fraction after depletion of Ctk1. Thus, we provide a catalogue of phosphoproteomic changes dependent on Ctk1. Taken together, our data suggest a stimulatory function of Ctk1 in 80S formation during translation initiation.
U2 - 10.1128/EC.00106-14
DO - 10.1128/EC.00106-14
M3 - Journal article
C2 - 25416238
VL - 14
SP - 86
JO - mSphere
JF - mSphere
SN - 2379-5042
IS - 1
ER -
ID: 128736574