Analytic framework for peptidomics applied to large-scale neuropeptide identification
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Analytic framework for peptidomics applied to large-scale neuropeptide identification. / Secher, Anna; Kelstrup, Christian D; Conde-Frieboes, Kilian W; Pyke, Charles; Raun, Kirsten; Wulff, Birgitte S; Olsen, Jesper V.
In: Nature Communications, Vol. 7, 11436, 2016.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Analytic framework for peptidomics applied to large-scale neuropeptide identification
AU - Secher, Anna
AU - Kelstrup, Christian D
AU - Conde-Frieboes, Kilian W
AU - Pyke, Charles
AU - Raun, Kirsten
AU - Wulff, Birgitte S
AU - Olsen, Jesper V
PY - 2016
Y1 - 2016
N2 - Large-scale mass spectrometry-based peptidomics for drug discovery is relatively unexplored because of challenges in peptide degradation and identification following tissue extraction. Here we present a streamlined analytical pipeline for large-scale peptidomics. We developed an optimized sample preparation protocol to achieve fast, reproducible and effective extraction of endogenous peptides from sub-dissected organs such as the brain, while diminishing unspecific protease activity. Each peptidome sample was analysed by high-resolution tandem mass spectrometry and the resulting data set was integrated with publically available databases. We developed and applied an algorithm that reduces the peptide complexity for identification of biologically relevant peptides. The developed pipeline was applied to rat hypothalamus and identifies thousands of neuropeptides and their post-translational modifications, which is combined in a resource format for visualization, qualitative and quantitative analyses.
AB - Large-scale mass spectrometry-based peptidomics for drug discovery is relatively unexplored because of challenges in peptide degradation and identification following tissue extraction. Here we present a streamlined analytical pipeline for large-scale peptidomics. We developed an optimized sample preparation protocol to achieve fast, reproducible and effective extraction of endogenous peptides from sub-dissected organs such as the brain, while diminishing unspecific protease activity. Each peptidome sample was analysed by high-resolution tandem mass spectrometry and the resulting data set was integrated with publically available databases. We developed and applied an algorithm that reduces the peptide complexity for identification of biologically relevant peptides. The developed pipeline was applied to rat hypothalamus and identifies thousands of neuropeptides and their post-translational modifications, which is combined in a resource format for visualization, qualitative and quantitative analyses.
U2 - 10.1038/ncomms11436
DO - 10.1038/ncomms11436
M3 - Journal article
C2 - 27142507
VL - 7
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
M1 - 11436
ER -
ID: 161081462