Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation

Research output: Contribution to journal › Journal article › Research › peer-review

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Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. / Mooser, Clémence; Symeonidou, Ioanna-Eleni; Leimbacher, Pia-Amata; Ribeiro, Alison; Shorrocks, Ann-Marie K; Jungmichel, Stephanie; Larsen, Sara C; Knechtle, Katja; Jasrotia, Arti; Zurbriggen, Diana; Jeanrenaud, Alain; Leikauf, Colin; Fink, Daniel; Nielsen, Michael L; Blackford, Andrew N; Stucki, Manuel.

In: Nature Communications, Vol. 11, 123, 2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Mooser, C, Symeonidou, I-E, Leimbacher, P-A, Ribeiro, A, Shorrocks, A-MK, Jungmichel, S, Larsen, SC, Knechtle, K, Jasrotia, A, Zurbriggen, D, Jeanrenaud, A, Leikauf, C, Fink, D, Nielsen, ML, Blackford, AN & Stucki, M 2020, 'Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation', Nature Communications, vol. 11, 123. https://doi.org/10.1038/s41467-019-13981-x

APA

Mooser, C., Symeonidou, I-E., Leimbacher, P-A., Ribeiro, A., Shorrocks, A-M. K., Jungmichel, S., Larsen, S. C., Knechtle, K., Jasrotia, A., Zurbriggen, D., Jeanrenaud, A., Leikauf, C., Fink, D., Nielsen, M. L., Blackford, A. N., & Stucki, M. (2020). Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nature Communications, 11, [123]. https://doi.org/10.1038/s41467-019-13981-x

Vancouver

Mooser C, Symeonidou I-E, Leimbacher P-A, Ribeiro A, Shorrocks A-MK, Jungmichel S et al. Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. Nature Communications. 2020;11. 123. https://doi.org/10.1038/s41467-019-13981-x

Author

Mooser, Clémence ; Symeonidou, Ioanna-Eleni ; Leimbacher, Pia-Amata ; Ribeiro, Alison ; Shorrocks, Ann-Marie K ; Jungmichel, Stephanie ; Larsen, Sara C ; Knechtle, Katja ; Jasrotia, Arti ; Zurbriggen, Diana ; Jeanrenaud, Alain ; Leikauf, Colin ; Fink, Daniel ; Nielsen, Michael L ; Blackford, Andrew N ; Stucki, Manuel. / Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation. In: Nature Communications. 2020 ; Vol. 11.

Bibtex

@article{074c90217f7e49cfafced636be586f69,
title = "Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation",
abstract = "Induction of DNA double-strand breaks (DSBs) in ribosomal DNA (rDNA) repeats is associated with ATM-dependent repression of ribosomal RNA synthesis and large-scale reorganization of nucleolar architecture, but the signaling events that regulate these responses are largely elusive. Here we show that the nucleolar response to rDNA breaks is dependent on both ATM and ATR activity. We further demonstrate that ATM- and NBS1-dependent recruitment of TOPBP1 in the nucleoli is required for inhibition of ribosomal RNA synthesis and nucleolar segregation in response to rDNA breaks. Mechanistically, TOPBP1 recruitment is mediated by phosphorylation-dependent interactions between three of its BRCT domains and conserved phosphorylated Ser/Thr residues at the C-terminus of the nucleolar phosphoprotein Treacle. Our data thus reveal an important cooperation between TOPBP1 and Treacle in the signaling cascade that triggers transcriptional inhibition and nucleolar segregation in response to rDNA breaks.",
author = "Cl{\'e}mence Mooser and Ioanna-Eleni Symeonidou and Pia-Amata Leimbacher and Alison Ribeiro and Shorrocks, {Ann-Marie K} and Stephanie Jungmichel and Larsen, {Sara C} and Katja Knechtle and Arti Jasrotia and Diana Zurbriggen and Alain Jeanrenaud and Colin Leikauf and Daniel Fink and Nielsen, {Michael L} and Blackford, {Andrew N} and Manuel Stucki",
year = "2020",
doi = "10.1038/s41467-019-13981-x",
language = "English",
volume = "11",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Treacle controls the nucleolar response to rDNA breaks via TOPBP1 recruitment and ATR activation

AU - Mooser, Clémence

AU - Symeonidou, Ioanna-Eleni

AU - Leimbacher, Pia-Amata

AU - Ribeiro, Alison

AU - Shorrocks, Ann-Marie K

AU - Jungmichel, Stephanie

AU - Larsen, Sara C

AU - Knechtle, Katja

AU - Jasrotia, Arti

AU - Zurbriggen, Diana

AU - Jeanrenaud, Alain

AU - Leikauf, Colin

AU - Fink, Daniel

AU - Nielsen, Michael L

AU - Blackford, Andrew N

AU - Stucki, Manuel

PY - 2020

Y1 - 2020

N2 - Induction of DNA double-strand breaks (DSBs) in ribosomal DNA (rDNA) repeats is associated with ATM-dependent repression of ribosomal RNA synthesis and large-scale reorganization of nucleolar architecture, but the signaling events that regulate these responses are largely elusive. Here we show that the nucleolar response to rDNA breaks is dependent on both ATM and ATR activity. We further demonstrate that ATM- and NBS1-dependent recruitment of TOPBP1 in the nucleoli is required for inhibition of ribosomal RNA synthesis and nucleolar segregation in response to rDNA breaks. Mechanistically, TOPBP1 recruitment is mediated by phosphorylation-dependent interactions between three of its BRCT domains and conserved phosphorylated Ser/Thr residues at the C-terminus of the nucleolar phosphoprotein Treacle. Our data thus reveal an important cooperation between TOPBP1 and Treacle in the signaling cascade that triggers transcriptional inhibition and nucleolar segregation in response to rDNA breaks.

AB - Induction of DNA double-strand breaks (DSBs) in ribosomal DNA (rDNA) repeats is associated with ATM-dependent repression of ribosomal RNA synthesis and large-scale reorganization of nucleolar architecture, but the signaling events that regulate these responses are largely elusive. Here we show that the nucleolar response to rDNA breaks is dependent on both ATM and ATR activity. We further demonstrate that ATM- and NBS1-dependent recruitment of TOPBP1 in the nucleoli is required for inhibition of ribosomal RNA synthesis and nucleolar segregation in response to rDNA breaks. Mechanistically, TOPBP1 recruitment is mediated by phosphorylation-dependent interactions between three of its BRCT domains and conserved phosphorylated Ser/Thr residues at the C-terminus of the nucleolar phosphoprotein Treacle. Our data thus reveal an important cooperation between TOPBP1 and Treacle in the signaling cascade that triggers transcriptional inhibition and nucleolar segregation in response to rDNA breaks.

U2 - 10.1038/s41467-019-13981-x

DO - 10.1038/s41467-019-13981-x

M3 - Journal article

C2 - 31913317

VL - 11

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 123

ER -

ID: 239208514