The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep
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The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep. / Noya, Sara B; Colameo, David; Brüning, Franziska; Spinnler, Andrea; Mircsof, Dennis; Opitz, Lennart; Mann, Matthias; Tyagarajan, Shiva K; Robles, Maria S; Brown, Steven A.
In: Science, Vol. 366, No. 6462, eaav2642 , 2019.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - The forebrain synaptic transcriptome is organized by clocks but its proteome is driven by sleep
AU - Noya, Sara B
AU - Colameo, David
AU - Brüning, Franziska
AU - Spinnler, Andrea
AU - Mircsof, Dennis
AU - Opitz, Lennart
AU - Mann, Matthias
AU - Tyagarajan, Shiva K
AU - Robles, Maria S
AU - Brown, Steven A
PY - 2019
Y1 - 2019
N2 - Neurons have adapted mechanisms to traffic RNA and protein into distant dendritic and axonal arbors. Taking a biochemical approach, we reveal that forebrain synaptic transcript accumulation shows overwhelmingly daily rhythms, with two-thirds of synaptic transcripts showing time-of-day-dependent abundance independent of oscillations in the soma. These transcripts formed two sharp temporal and functional clusters, with transcripts preceding dawn related to metabolism and translation and those anticipating dusk related to synaptic transmission. Characterization of the synaptic proteome around the clock demonstrates the functional relevance of temporal gating for synaptic processes and energy homeostasis. Unexpectedly, sleep deprivation completely abolished proteome but not transcript oscillations. Altogether, the emerging picture is one of a circadian anticipation of messenger RNA needs in the synapse followed by translation as demanded by sleep-wake cycles.
AB - Neurons have adapted mechanisms to traffic RNA and protein into distant dendritic and axonal arbors. Taking a biochemical approach, we reveal that forebrain synaptic transcript accumulation shows overwhelmingly daily rhythms, with two-thirds of synaptic transcripts showing time-of-day-dependent abundance independent of oscillations in the soma. These transcripts formed two sharp temporal and functional clusters, with transcripts preceding dawn related to metabolism and translation and those anticipating dusk related to synaptic transmission. Characterization of the synaptic proteome around the clock demonstrates the functional relevance of temporal gating for synaptic processes and energy homeostasis. Unexpectedly, sleep deprivation completely abolished proteome but not transcript oscillations. Altogether, the emerging picture is one of a circadian anticipation of messenger RNA needs in the synapse followed by translation as demanded by sleep-wake cycles.
U2 - 10.1126/science.aav2642
DO - 10.1126/science.aav2642
M3 - Journal article
C2 - 31601739
VL - 366
JO - Science
JF - Science
SN - 0036-8075
IS - 6462
M1 - eaav2642
ER -
ID: 229855390