Social network architecture of human immune cells unveiled by quantitative proteomics
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Social network architecture of human immune cells unveiled by quantitative proteomics. / Rieckmann, Jan C; Geiger, Roger; Hornburg, Daniel; Wolf, Tobias; Kveler, Ksenya; Jarrossay, David; Sallusto, Federica; Shen-Orr, Shai S; Lanzavecchia, Antonio; Mann, Matthias; Meissner, Felix.
In: Nature Immunology, Vol. 18, No. 5, 05.2017, p. 583-593.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Social network architecture of human immune cells unveiled by quantitative proteomics
AU - Rieckmann, Jan C
AU - Geiger, Roger
AU - Hornburg, Daniel
AU - Wolf, Tobias
AU - Kveler, Ksenya
AU - Jarrossay, David
AU - Sallusto, Federica
AU - Shen-Orr, Shai S
AU - Lanzavecchia, Antonio
AU - Mann, Matthias
AU - Meissner, Felix
PY - 2017/5
Y1 - 2017/5
N2 - The immune system is unique in its dynamic interplay between numerous cell types. However, a system-wide view of how immune cells communicate to protect against disease has not yet been established. We applied high-resolution mass-spectrometry-based proteomics to characterize 28 primary human hematopoietic cell populations in steady and activated states at a depth of >10,000 proteins in total. Protein copy numbers revealed a specialization of immune cells for ligand and receptor expression, thereby connecting distinct immune functions. By integrating total and secreted proteomes, we discovered fundamental intercellular communication structures and previously unknown connections between cell types. Our publicly accessible (http://www.immprot.org/) proteomic resource provides a framework for the orchestration of cellular interplay and a reference for altered communication associated with pathology.
AB - The immune system is unique in its dynamic interplay between numerous cell types. However, a system-wide view of how immune cells communicate to protect against disease has not yet been established. We applied high-resolution mass-spectrometry-based proteomics to characterize 28 primary human hematopoietic cell populations in steady and activated states at a depth of >10,000 proteins in total. Protein copy numbers revealed a specialization of immune cells for ligand and receptor expression, thereby connecting distinct immune functions. By integrating total and secreted proteomes, we discovered fundamental intercellular communication structures and previously unknown connections between cell types. Our publicly accessible (http://www.immprot.org/) proteomic resource provides a framework for the orchestration of cellular interplay and a reference for altered communication associated with pathology.
KW - Animals
KW - Blood Cells
KW - Bodily Secretions
KW - Cell Communication
KW - Computer Simulation
KW - Humans
KW - Immunity, Cellular
KW - Mass Spectrometry
KW - Protein Interaction Maps
KW - Proteome
KW - Proteomics
KW - Social Support
KW - Journal Article
U2 - 10.1038/ni.3693
DO - 10.1038/ni.3693
M3 - Journal article
C2 - 28263321
VL - 18
SP - 583
EP - 593
JO - Nature Immunology
JF - Nature Immunology
SN - 1529-2908
IS - 5
ER -
ID: 184292224