Quantitative proteomic assessment of very early cellular signaling events

Research output: Contribution to journal › Journal article › Research › peer-review

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Quantitative proteomic assessment of very early cellular signaling events. / Dengjel, Joern; Akimov, Vyacheslav; Olsen, Jesper Velgaard; Bunkenborg, Jakob; Mann, Matthias; Blagoev, Blagoy; Andersen, Jens S.

In: Nature Biotechnology, Vol. 25, No. 5, 2007, p. 566-8.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Dengjel, J, Akimov, V, Olsen, JV, Bunkenborg, J, Mann, M, Blagoev, B & Andersen, JS 2007, 'Quantitative proteomic assessment of very early cellular signaling events', Nature Biotechnology, vol. 25, no. 5, pp. 566-8. https://doi.org/10.1038/nbt1301

APA

Dengjel, J., Akimov, V., Olsen, J. V., Bunkenborg, J., Mann, M., Blagoev, B., & Andersen, J. S. (2007). Quantitative proteomic assessment of very early cellular signaling events. Nature Biotechnology, 25(5), 566-8. https://doi.org/10.1038/nbt1301

Vancouver

Dengjel J, Akimov V, Olsen JV, Bunkenborg J, Mann M, Blagoev B et al. Quantitative proteomic assessment of very early cellular signaling events. Nature Biotechnology. 2007;25(5):566-8. https://doi.org/10.1038/nbt1301

Author

Dengjel, Joern ; Akimov, Vyacheslav ; Olsen, Jesper Velgaard ; Bunkenborg, Jakob ; Mann, Matthias ; Blagoev, Blagoy ; Andersen, Jens S. / Quantitative proteomic assessment of very early cellular signaling events. In: Nature Biotechnology. 2007 ; Vol. 25, No. 5. pp. 566-8.

Bibtex

@article{46f571dcdf16488cbf34533e8ce4cf37,
title = "Quantitative proteomic assessment of very early cellular signaling events",
abstract = "Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.",
author = "Joern Dengjel and Vyacheslav Akimov and Olsen, {Jesper Velgaard} and Jakob Bunkenborg and Matthias Mann and Blagoy Blagoev and Andersen, {Jens S}",
note = "Keywords: Fluorescence Recovery After Photobleaching; Metabolic Clearance Rate; Microscopy, Fluorescence; Phosphorylation; Proteome; Receptor, Epidermal Growth Factor; Signal Transduction; Time Factors",
year = "2007",
doi = "10.1038/nbt1301",
language = "English",
volume = "25",
pages = "566--8",
journal = "Nature Biotechnology",
issn = "1087-0156",
publisher = "nature publishing group",
number = "5",

}

RIS

TY - JOUR

T1 - Quantitative proteomic assessment of very early cellular signaling events

AU - Dengjel, Joern

AU - Akimov, Vyacheslav

AU - Olsen, Jesper Velgaard

AU - Bunkenborg, Jakob

AU - Mann, Matthias

AU - Blagoev, Blagoy

AU - Andersen, Jens S

N1 - Keywords: Fluorescence Recovery After Photobleaching; Metabolic Clearance Rate; Microscopy, Fluorescence; Phosphorylation; Proteome; Receptor, Epidermal Growth Factor; Signal Transduction; Time Factors

PY - 2007

Y1 - 2007

N2 - Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

AB - Technical limitations have prevented proteomic analyses of events occurring less than 30 s after signal initiation. We developed an automated, continuous quench-flow system allowing quantitative proteomic assessment of very early cellular signaling events (qPACE) with a time resolution of 1 s. Using this technique, we determined that autophosphorylation of the epidermal growth factor receptor occurs within 1 s after ligand stimulation and is followed rapidly by phosphorylation of the downstream signaling intermediates Src homologous and collagen-like protein and phospholipase C gamma 1.

U2 - 10.1038/nbt1301

DO - 10.1038/nbt1301

M3 - Journal article

C2 - 17450129

VL - 25

SP - 566

EP - 568

JO - Nature Biotechnology

JF - Nature Biotechnology

SN - 1087-0156

IS - 5

ER -

ID: 46461904