PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex

Research output: Contribution to journal › Journal article › Research › peer-review

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PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. / Link, Stephanie; Spitzer, Ramona M M; Sana, Maryam; Torrado, Mario; Völker-Albert, Moritz C; Keilhauer, Eva C; Burgold, Thomas; Pünzeler, Sebastian; Low, Jason K K; Lindström, Ida; Nist, Andrea; Regnard, Catherine; Stiewe, Thorsten; Hendrich, Brian; Imhof, Axel; Mann, Matthias; Mackay, Joel P; Bartkuhn, Marek; Hake, Sandra B.

In: Nature Communications, Vol. 9, No. 1, 16.10.2018, p. 4300.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Link, S, Spitzer, RMM, Sana, M, Torrado, M, Völker-Albert, MC, Keilhauer, EC, Burgold, T, Pünzeler, S, Low, JKK, Lindström, I, Nist, A, Regnard, C, Stiewe, T, Hendrich, B, Imhof, A, Mann, M, Mackay, JP, Bartkuhn, M & Hake, SB 2018, 'PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex', Nature Communications, vol. 9, no. 1, pp. 4300. https://doi.org/10.1038/s41467-018-06665-5

APA

Link, S., Spitzer, R. M. M., Sana, M., Torrado, M., Völker-Albert, M. C., Keilhauer, E. C., Burgold, T., Pünzeler, S., Low, J. K. K., Lindström, I., Nist, A., Regnard, C., Stiewe, T., Hendrich, B., Imhof, A., Mann, M., Mackay, J. P., Bartkuhn, M., & Hake, S. B. (2018). PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nature Communications, 9(1), 4300. https://doi.org/10.1038/s41467-018-06665-5

Vancouver

Link S, Spitzer RMM, Sana M, Torrado M, Völker-Albert MC, Keilhauer EC et al. PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. Nature Communications. 2018 Oct 16;9(1):4300. https://doi.org/10.1038/s41467-018-06665-5

Author

Link, Stephanie ; Spitzer, Ramona M M ; Sana, Maryam ; Torrado, Mario ; Völker-Albert, Moritz C ; Keilhauer, Eva C ; Burgold, Thomas ; Pünzeler, Sebastian ; Low, Jason K K ; Lindström, Ida ; Nist, Andrea ; Regnard, Catherine ; Stiewe, Thorsten ; Hendrich, Brian ; Imhof, Axel ; Mann, Matthias ; Mackay, Joel P ; Bartkuhn, Marek ; Hake, Sandra B. / PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex. In: Nature Communications. 2018 ; Vol. 9, No. 1. pp. 4300.

Bibtex

@article{803c60566ed04c5096f20e63bbc052f4,
title = "PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex",
abstract = "Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified that PWWP2A tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial-facial development. Here, using in vitro assays, we show that distinct domains of PWWP2A mediate binding to free linker DNA as well as H3K36me3 nucleosomes. In vivo, PWWP2A strongly recognizes H2A.Z-containing regulatory regions and weakly binds H3K36me3-containing gene bodies. Further, PWWP2A binds to an MTA1-specific subcomplex of the NuRD complex (M1HR), which consists solely of MTA1, HDAC1, and RBBP4/7, and excludes CHD, GATAD2 and MBD proteins. Depletion of PWWP2A leads to an increase of acetylation levels on H3K27 as well as H2A.Z, presumably by impaired chromatin recruitment of M1HR. Thus, this study identifies PWWP2A as a complex chromatin-binding protein that serves to direct the deacetylase complex M1HR to H2A.Z-containing chromatin, thereby promoting changes in histone acetylation levels.",
author = "Stephanie Link and Spitzer, {Ramona M M} and Maryam Sana and Mario Torrado and V{\"o}lker-Albert, {Moritz C} and Keilhauer, {Eva C} and Thomas Burgold and Sebastian P{\"u}nzeler and Low, {Jason K K} and Ida Lindstr{\"o}m and Andrea Nist and Catherine Regnard and Thorsten Stiewe and Brian Hendrich and Axel Imhof and Matthias Mann and Mackay, {Joel P} and Marek Bartkuhn and Hake, {Sandra B}",
year = "2018",
month = oct,
day = "16",
doi = "10.1038/s41467-018-06665-5",
language = "English",
volume = "9",
pages = "4300",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - PWWP2A binds distinct chromatin moieties and interacts with an MTA1-specific core NuRD complex

AU - Link, Stephanie

AU - Spitzer, Ramona M M

AU - Sana, Maryam

AU - Torrado, Mario

AU - Völker-Albert, Moritz C

AU - Keilhauer, Eva C

AU - Burgold, Thomas

AU - Pünzeler, Sebastian

AU - Low, Jason K K

AU - Lindström, Ida

AU - Nist, Andrea

AU - Regnard, Catherine

AU - Stiewe, Thorsten

AU - Hendrich, Brian

AU - Imhof, Axel

AU - Mann, Matthias

AU - Mackay, Joel P

AU - Bartkuhn, Marek

AU - Hake, Sandra B

PY - 2018/10/16

Y1 - 2018/10/16

N2 - Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified that PWWP2A tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial-facial development. Here, using in vitro assays, we show that distinct domains of PWWP2A mediate binding to free linker DNA as well as H3K36me3 nucleosomes. In vivo, PWWP2A strongly recognizes H2A.Z-containing regulatory regions and weakly binds H3K36me3-containing gene bodies. Further, PWWP2A binds to an MTA1-specific subcomplex of the NuRD complex (M1HR), which consists solely of MTA1, HDAC1, and RBBP4/7, and excludes CHD, GATAD2 and MBD proteins. Depletion of PWWP2A leads to an increase of acetylation levels on H3K27 as well as H2A.Z, presumably by impaired chromatin recruitment of M1HR. Thus, this study identifies PWWP2A as a complex chromatin-binding protein that serves to direct the deacetylase complex M1HR to H2A.Z-containing chromatin, thereby promoting changes in histone acetylation levels.

AB - Chromatin structure and function is regulated by reader proteins recognizing histone modifications and/or histone variants. We recently identified that PWWP2A tightly binds to H2A.Z-containing nucleosomes and is involved in mitotic progression and cranial-facial development. Here, using in vitro assays, we show that distinct domains of PWWP2A mediate binding to free linker DNA as well as H3K36me3 nucleosomes. In vivo, PWWP2A strongly recognizes H2A.Z-containing regulatory regions and weakly binds H3K36me3-containing gene bodies. Further, PWWP2A binds to an MTA1-specific subcomplex of the NuRD complex (M1HR), which consists solely of MTA1, HDAC1, and RBBP4/7, and excludes CHD, GATAD2 and MBD proteins. Depletion of PWWP2A leads to an increase of acetylation levels on H3K27 as well as H2A.Z, presumably by impaired chromatin recruitment of M1HR. Thus, this study identifies PWWP2A as a complex chromatin-binding protein that serves to direct the deacetylase complex M1HR to H2A.Z-containing chromatin, thereby promoting changes in histone acetylation levels.

U2 - 10.1038/s41467-018-06665-5

DO - 10.1038/s41467-018-06665-5

M3 - Journal article

C2 - 30327463

VL - 9

SP - 4300

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -

ID: 203882742