Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer

Research output: Contribution to journal › Journal article › Research › peer-review

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Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer. / Wiebringhaus, Robert; Pecoraro, Matteo; Neubauer, Heidi A; Trachtová, Karolína; Trimmel, Bettina; Wieselberg, Maritta; Pencik, Jan; Egger, Gerda; Krall, Christoph; Moriggl, Richard; Mann, Matthias; Hantusch, Brigitte; Kenner, Lukas.

In: Cancers, Vol. 13, No. 23, 30.11.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wiebringhaus, R, Pecoraro, M, Neubauer, HA, Trachtová, K, Trimmel, B, Wieselberg, M, Pencik, J, Egger, G, Krall, C, Moriggl, R, Mann, M, Hantusch, B & Kenner, L 2021, 'Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer', Cancers, vol. 13, no. 23. https://doi.org/10.3390/cancers13236036

APA

Wiebringhaus, R., Pecoraro, M., Neubauer, H. A., Trachtová, K., Trimmel, B., Wieselberg, M., Pencik, J., Egger, G., Krall, C., Moriggl, R., Mann, M., Hantusch, B., & Kenner, L. (2021). Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer. Cancers, 13(23). https://doi.org/10.3390/cancers13236036

Vancouver

Wiebringhaus R, Pecoraro M, Neubauer HA, Trachtová K, Trimmel B, Wieselberg M et al. Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer. Cancers. 2021 Nov 30;13(23). https://doi.org/10.3390/cancers13236036

Author

Wiebringhaus, Robert ; Pecoraro, Matteo ; Neubauer, Heidi A ; Trachtová, Karolína ; Trimmel, Bettina ; Wieselberg, Maritta ; Pencik, Jan ; Egger, Gerda ; Krall, Christoph ; Moriggl, Richard ; Mann, Matthias ; Hantusch, Brigitte ; Kenner, Lukas. / Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer. In: Cancers. 2021 ; Vol. 13, No. 23.

Bibtex

@article{c11ea70450414551bb88ee838c225d3a,
title = "Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer",
abstract = "We aimed to identify novel markers for aggressive prostate cancer in a STAT3-low proteomics-derived dataset of mitochondrial proteins by immunohistochemical analysis and correlation with transcriptomic data and biochemical recurrence in a STAT3 independent PCa cohort. Formalin-fixed paraffin-embedded tissue (FFPE) sample selection for proteomic analysis and tissue-microarray (TMA) generation was conducted from a cohort of PCa patients. Retrospective data analysis was performed with the same cohort. 153 proteins differentially expressed between STAT3-low and STAT3-high samples were identified. Out of these, 46 proteins were associated with mitochondrial processes including oxidative phosphorylation (OXPHOS), and 45 proteins were upregulated, including NDUFS1/ATP5O. In a STAT3 independent PCa cohort, high expression of NDUFS1/ATP5O was confirmed by immunocytochemistry (IHC) and was significantly associated with earlier biochemical recurrence (BCR). mRNA expression levels for these two genes were significantly higher in intra-epithelial neoplasia and in PCa compared to benign prostate glands. NDUFS1/ATP5O levels are increased both at the mRNA and protein level in aggressive PCa. Our results provide evidence that NDUFS1/ATP5O could be used to identify high-risk PCa patients.",
author = "Robert Wiebringhaus and Matteo Pecoraro and Neubauer, {Heidi A} and Karol{\'i}na Trachtov{\'a} and Bettina Trimmel and Maritta Wieselberg and Jan Pencik and Gerda Egger and Christoph Krall and Richard Moriggl and Matthias Mann and Brigitte Hantusch and Lukas Kenner",
year = "2021",
month = nov,
day = "30",
doi = "10.3390/cancers13236036",
language = "English",
volume = "13",
journal = "Cancers",
issn = "2072-6694",
publisher = "M D P I AG",
number = "23",

}

RIS

TY - JOUR

T1 - Proteomic Analysis Identifies NDUFS1 and ATP5O as Novel Markers for Survival Outcome in Prostate Cancer

AU - Wiebringhaus, Robert

AU - Pecoraro, Matteo

AU - Neubauer, Heidi A

AU - Trachtová, Karolína

AU - Trimmel, Bettina

AU - Wieselberg, Maritta

AU - Pencik, Jan

AU - Egger, Gerda

AU - Krall, Christoph

AU - Moriggl, Richard

AU - Mann, Matthias

AU - Hantusch, Brigitte

AU - Kenner, Lukas

PY - 2021/11/30

Y1 - 2021/11/30

N2 - We aimed to identify novel markers for aggressive prostate cancer in a STAT3-low proteomics-derived dataset of mitochondrial proteins by immunohistochemical analysis and correlation with transcriptomic data and biochemical recurrence in a STAT3 independent PCa cohort. Formalin-fixed paraffin-embedded tissue (FFPE) sample selection for proteomic analysis and tissue-microarray (TMA) generation was conducted from a cohort of PCa patients. Retrospective data analysis was performed with the same cohort. 153 proteins differentially expressed between STAT3-low and STAT3-high samples were identified. Out of these, 46 proteins were associated with mitochondrial processes including oxidative phosphorylation (OXPHOS), and 45 proteins were upregulated, including NDUFS1/ATP5O. In a STAT3 independent PCa cohort, high expression of NDUFS1/ATP5O was confirmed by immunocytochemistry (IHC) and was significantly associated with earlier biochemical recurrence (BCR). mRNA expression levels for these two genes were significantly higher in intra-epithelial neoplasia and in PCa compared to benign prostate glands. NDUFS1/ATP5O levels are increased both at the mRNA and protein level in aggressive PCa. Our results provide evidence that NDUFS1/ATP5O could be used to identify high-risk PCa patients.

AB - We aimed to identify novel markers for aggressive prostate cancer in a STAT3-low proteomics-derived dataset of mitochondrial proteins by immunohistochemical analysis and correlation with transcriptomic data and biochemical recurrence in a STAT3 independent PCa cohort. Formalin-fixed paraffin-embedded tissue (FFPE) sample selection for proteomic analysis and tissue-microarray (TMA) generation was conducted from a cohort of PCa patients. Retrospective data analysis was performed with the same cohort. 153 proteins differentially expressed between STAT3-low and STAT3-high samples were identified. Out of these, 46 proteins were associated with mitochondrial processes including oxidative phosphorylation (OXPHOS), and 45 proteins were upregulated, including NDUFS1/ATP5O. In a STAT3 independent PCa cohort, high expression of NDUFS1/ATP5O was confirmed by immunocytochemistry (IHC) and was significantly associated with earlier biochemical recurrence (BCR). mRNA expression levels for these two genes were significantly higher in intra-epithelial neoplasia and in PCa compared to benign prostate glands. NDUFS1/ATP5O levels are increased both at the mRNA and protein level in aggressive PCa. Our results provide evidence that NDUFS1/ATP5O could be used to identify high-risk PCa patients.

U2 - 10.3390/cancers13236036

DO - 10.3390/cancers13236036

M3 - Journal article

C2 - 34885151

VL - 13

JO - Cancers

JF - Cancers

SN - 2072-6694

IS - 23

ER -

ID: 303114796