Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease

Research output: Contribution to journal › Journal article › Research › peer-review

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Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease. / Karayel, Ozge; Virreira Winter, Sebastian; Padmanabhan, Shalini; Kuras, Yuliya I.; Vu, Duc Tung; Tuncali, Idil; Merchant, Kalpana; Wills, Anne Marie; Scherzer, Clemens R.; Mann, Matthias.

In: Cell Reports Medicine, Vol. 3, No. 6, 100661, 2022.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Karayel, O, Virreira Winter, S, Padmanabhan, S, Kuras, YI, Vu, DT, Tuncali, I, Merchant, K, Wills, AM, Scherzer, CR & Mann, M 2022, 'Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease', Cell Reports Medicine, vol. 3, no. 6, 100661. https://doi.org/10.1016/j.xcrm.2022.100661

APA

Karayel, O., Virreira Winter, S., Padmanabhan, S., Kuras, Y. I., Vu, D. T., Tuncali, I., Merchant, K., Wills, A. M., Scherzer, C. R., & Mann, M. (2022). Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease. Cell Reports Medicine, 3(6), [100661]. https://doi.org/10.1016/j.xcrm.2022.100661

Vancouver

Karayel O, Virreira Winter S, Padmanabhan S, Kuras YI, Vu DT, Tuncali I et al. Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease. Cell Reports Medicine. 2022;3(6). 100661. https://doi.org/10.1016/j.xcrm.2022.100661

Author

Karayel, Ozge ; Virreira Winter, Sebastian ; Padmanabhan, Shalini ; Kuras, Yuliya I. ; Vu, Duc Tung ; Tuncali, Idil ; Merchant, Kalpana ; Wills, Anne Marie ; Scherzer, Clemens R. ; Mann, Matthias. / Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease. In: Cell Reports Medicine. 2022 ; Vol. 3, No. 6.

Bibtex

@article{7626ed81e757410ab4f19acf43bb94b4,
title = "Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease",
abstract = "Parkinson's disease (PD) is a growing burden worldwide, and there is no reliable biomarker used in clinical routines to date. Cerebrospinal fluid (CSF) is routinely collected in patients with neurological symptoms and should closely reflect alterations in PD patients{\textquoteright} brains. Here, we describe a scalable and sensitive mass spectrometry (MS)-based proteomics workflow for CSF proteome profiling. From two independent cohorts with over 200 individuals, our workflow reproducibly quantifies over 1,700 proteins from minimal CSF amounts. Machine learning determines OMD, CD44, VGF, PRL, and MAN2B1 to be altered in PD patients or to significantly correlate with clinical scores. We also uncover signatures of enhanced neuroinflammation in LRRK2 G2019S carriers, as indicated by increased levels of CTSS, PLD4, and HLA proteins. A comparison with our previously acquired urinary proteomes reveals a large overlap in PD-associated changes, including lysosomal proteins, opening up new avenues to improve our understanding of PD pathogenesis.",
keywords = "biomarker, CSF, DIA, LRRK2, mass spectrometry, Parkinson's disease, proteomics",
author = "Ozge Karayel and {Virreira Winter}, Sebastian and Shalini Padmanabhan and Kuras, {Yuliya I.} and Vu, {Duc Tung} and Idil Tuncali and Kalpana Merchant and Wills, {Anne Marie} and Scherzer, {Clemens R.} and Matthias Mann",
note = "Publisher Copyright: {\textcopyright} 2022 The Author(s)",
year = "2022",
doi = "10.1016/j.xcrm.2022.100661",
language = "English",
volume = "3",
journal = "Cell Reports Medicine",
issn = "2666-3791",
publisher = "Cell Press",
number = "6",

}

RIS

TY - JOUR

T1 - Proteome profiling of cerebrospinal fluid reveals biomarker candidates for Parkinson's disease

AU - Karayel, Ozge

AU - Virreira Winter, Sebastian

AU - Padmanabhan, Shalini

AU - Kuras, Yuliya I.

AU - Vu, Duc Tung

AU - Tuncali, Idil

AU - Merchant, Kalpana

AU - Wills, Anne Marie

AU - Scherzer, Clemens R.

AU - Mann, Matthias

N1 - Publisher Copyright: © 2022 The Author(s)

PY - 2022

Y1 - 2022

N2 - Parkinson's disease (PD) is a growing burden worldwide, and there is no reliable biomarker used in clinical routines to date. Cerebrospinal fluid (CSF) is routinely collected in patients with neurological symptoms and should closely reflect alterations in PD patients’ brains. Here, we describe a scalable and sensitive mass spectrometry (MS)-based proteomics workflow for CSF proteome profiling. From two independent cohorts with over 200 individuals, our workflow reproducibly quantifies over 1,700 proteins from minimal CSF amounts. Machine learning determines OMD, CD44, VGF, PRL, and MAN2B1 to be altered in PD patients or to significantly correlate with clinical scores. We also uncover signatures of enhanced neuroinflammation in LRRK2 G2019S carriers, as indicated by increased levels of CTSS, PLD4, and HLA proteins. A comparison with our previously acquired urinary proteomes reveals a large overlap in PD-associated changes, including lysosomal proteins, opening up new avenues to improve our understanding of PD pathogenesis.

AB - Parkinson's disease (PD) is a growing burden worldwide, and there is no reliable biomarker used in clinical routines to date. Cerebrospinal fluid (CSF) is routinely collected in patients with neurological symptoms and should closely reflect alterations in PD patients’ brains. Here, we describe a scalable and sensitive mass spectrometry (MS)-based proteomics workflow for CSF proteome profiling. From two independent cohorts with over 200 individuals, our workflow reproducibly quantifies over 1,700 proteins from minimal CSF amounts. Machine learning determines OMD, CD44, VGF, PRL, and MAN2B1 to be altered in PD patients or to significantly correlate with clinical scores. We also uncover signatures of enhanced neuroinflammation in LRRK2 G2019S carriers, as indicated by increased levels of CTSS, PLD4, and HLA proteins. A comparison with our previously acquired urinary proteomes reveals a large overlap in PD-associated changes, including lysosomal proteins, opening up new avenues to improve our understanding of PD pathogenesis.

KW - biomarker

KW - CSF

KW - DIA

KW - LRRK2

KW - mass spectrometry

KW - Parkinson's disease

KW - proteomics

U2 - 10.1016/j.xcrm.2022.100661

DO - 10.1016/j.xcrm.2022.100661

M3 - Journal article

C2 - 35732154

AN - SCOPUS:85132594235

VL - 3

JO - Cell Reports Medicine

JF - Cell Reports Medicine

SN - 2666-3791

IS - 6

M1 - 100661

ER -

ID: 312700472