Plasma Proteome Profiling to Assess Human Health and Disease

Research output: Contribution to journalJournal articleResearchpeer-review

  • Philipp E Geyer
  • Nils A Kulak
  • Garwin Pichler
  • Lesca M Holdt
  • Daniel Teupser
  • Mann, Matthias

Proteins in the circulatory system mirror an individual's physiology. In daily clinical practice, protein levels are generally determined using single-protein immunoassays. High-throughput, quantitative analysis using mass-spectrometry-based proteomics of blood, plasma, and serum would be advantageous but is challenging because of the high dynamic range of protein abundances. Here, we introduce a rapid and robust "plasma proteome profiling" pipeline. This single-run shotgun proteomic workflow does not require protein depletion and enables quantitative analysis of hundreds of plasma proteomes from 1 μl single finger pricks with 20 min gradients. The apolipoprotein family, inflammatory markers such as C-reactive protein, gender-related proteins, and >40 FDA-approved biomarkers are reproducibly quantified (CV <20% with label-free quantification). Furthermore, we functionally interpret a 1,000-protein, quantitative plasma proteome obtained by simple peptide pre-fractionation. Plasma proteome profiling delivers an informative portrait of a person's health state, and we envision its large-scale use in biomedicine.

Original languageEnglish
JournalCell Systems
Issue number3
Pages (from-to)185-95
Number of pages11
Publication statusPublished - 23 Mar 2016

    Research areas

  • Journal Article

ID: 165939283