Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency

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Standard

Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency. / Yang, Pengyi; Humphrey, Sean J; Cinghu, Senthilkumar; Pathania, Rajneesh; Oldfield, Andrew J; Kumar, Dhirendra; Perera, Dinuka; Yang, Jean Y H; James, David E; Mann, Matthias; Jothi, Raja.

In: Cell Systems, Vol. 8, No. 5, 22.05.2019, p. 427-445.e10.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Yang, P, Humphrey, SJ, Cinghu, S, Pathania, R, Oldfield, AJ, Kumar, D, Perera, D, Yang, JYH, James, DE, Mann, M & Jothi, R 2019, 'Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency', Cell Systems, vol. 8, no. 5, pp. 427-445.e10. https://doi.org/10.1016/j.cels.2019.03.012

APA

Yang, P., Humphrey, S. J., Cinghu, S., Pathania, R., Oldfield, A. J., Kumar, D., Perera, D., Yang, J. Y. H., James, D. E., Mann, M., & Jothi, R. (2019). Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency. Cell Systems, 8(5), 427-445.e10. https://doi.org/10.1016/j.cels.2019.03.012

Vancouver

Yang P, Humphrey SJ, Cinghu S, Pathania R, Oldfield AJ, Kumar D et al. Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency. Cell Systems. 2019 May 22;8(5):427-445.e10. https://doi.org/10.1016/j.cels.2019.03.012

Author

Yang, Pengyi ; Humphrey, Sean J ; Cinghu, Senthilkumar ; Pathania, Rajneesh ; Oldfield, Andrew J ; Kumar, Dhirendra ; Perera, Dinuka ; Yang, Jean Y H ; James, David E ; Mann, Matthias ; Jothi, Raja. / Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency. In: Cell Systems. 2019 ; Vol. 8, No. 5. pp. 427-445.e10.

Bibtex

@article{1312a969cdc5497bb6fc06c657fc300d,
title = "Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency",
abstract = "Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains incomplete. Here, we dissect the dynamics of pluripotent state transitions underlying pre- to post-implantation epiblast differentiation. Through comprehensive mapping of the proteome, phosphoproteome, transcriptome, and epigenome of embryonic stem cells transitioning from naive to primed pluripotency, we find that rapid, acute, and widespread changes to the phosphoproteome precede ordered changes to the epigenome, transcriptome, and proteome. Reconstruction of the kinase-substrate networks reveals signaling cascades, dynamics, and crosstalk. Distinct waves of global proteomic changes mark discrete phases of pluripotency, with cell-state-specific surface markers tracking pluripotent state transitions. Our data provide new insights into multi-layered control of the phased progression of pluripotency and a foundation for modeling mechanisms regulating pluripotent state transitions (www.stemcellatlas.org).",
author = "Pengyi Yang and Humphrey, {Sean J} and Senthilkumar Cinghu and Rajneesh Pathania and Oldfield, {Andrew J} and Dhirendra Kumar and Dinuka Perera and Yang, {Jean Y H} and James, {David E} and Matthias Mann and Raja Jothi",
note = "Copyright {\textcopyright} 2019 The Author(s). Published by Elsevier Inc. All rights reserved.",
year = "2019",
month = may,
day = "22",
doi = "10.1016/j.cels.2019.03.012",
language = "English",
volume = "8",
pages = "427--445.e10",
journal = "Cell Systems",
issn = "2405-4712",
publisher = "Cell Press",
number = "5",

}

RIS

TY - JOUR

T1 - Multi-omic Profiling Reveals Dynamics of the Phased Progression of Pluripotency

AU - Yang, Pengyi

AU - Humphrey, Sean J

AU - Cinghu, Senthilkumar

AU - Pathania, Rajneesh

AU - Oldfield, Andrew J

AU - Kumar, Dhirendra

AU - Perera, Dinuka

AU - Yang, Jean Y H

AU - James, David E

AU - Mann, Matthias

AU - Jothi, Raja

N1 - Copyright © 2019 The Author(s). Published by Elsevier Inc. All rights reserved.

PY - 2019/5/22

Y1 - 2019/5/22

N2 - Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains incomplete. Here, we dissect the dynamics of pluripotent state transitions underlying pre- to post-implantation epiblast differentiation. Through comprehensive mapping of the proteome, phosphoproteome, transcriptome, and epigenome of embryonic stem cells transitioning from naive to primed pluripotency, we find that rapid, acute, and widespread changes to the phosphoproteome precede ordered changes to the epigenome, transcriptome, and proteome. Reconstruction of the kinase-substrate networks reveals signaling cascades, dynamics, and crosstalk. Distinct waves of global proteomic changes mark discrete phases of pluripotency, with cell-state-specific surface markers tracking pluripotent state transitions. Our data provide new insights into multi-layered control of the phased progression of pluripotency and a foundation for modeling mechanisms regulating pluripotent state transitions (www.stemcellatlas.org).

AB - Pluripotency is highly dynamic and progresses through a continuum of pluripotent stem cell states. The two states that bookend the pluripotency continuum, naive and primed, are well characterized, but our understanding of the intermediate states and transitions between them remains incomplete. Here, we dissect the dynamics of pluripotent state transitions underlying pre- to post-implantation epiblast differentiation. Through comprehensive mapping of the proteome, phosphoproteome, transcriptome, and epigenome of embryonic stem cells transitioning from naive to primed pluripotency, we find that rapid, acute, and widespread changes to the phosphoproteome precede ordered changes to the epigenome, transcriptome, and proteome. Reconstruction of the kinase-substrate networks reveals signaling cascades, dynamics, and crosstalk. Distinct waves of global proteomic changes mark discrete phases of pluripotency, with cell-state-specific surface markers tracking pluripotent state transitions. Our data provide new insights into multi-layered control of the phased progression of pluripotency and a foundation for modeling mechanisms regulating pluripotent state transitions (www.stemcellatlas.org).

U2 - 10.1016/j.cels.2019.03.012

DO - 10.1016/j.cels.2019.03.012

M3 - Journal article

C2 - 31078527

VL - 8

SP - 427-445.e10

JO - Cell Systems

JF - Cell Systems

SN - 2405-4712

IS - 5

ER -

ID: 219531644