Identification of the transcription factor MAZ as a regulator of erythropoiesis

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Identification of the transcription factor MAZ as a regulator of erythropoiesis. / Deen, Darya; Butter, Falk; Daniels, Deborah E; Ferrer-Vicens, Ivan; Ferguson, Daniel C J; Holland, Michelle L; Samara, Vasiliki; Sloane-Stanley, Jacqueline A; Ayyub, Helena; Mann, Matthias; Frayne, Jan; Garrick, David; Vernimmen, Douglas.

In: Blood advances, Vol. 5, No. 15, 10.08.2021, p. 3002-3015.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Deen, D, Butter, F, Daniels, DE, Ferrer-Vicens, I, Ferguson, DCJ, Holland, ML, Samara, V, Sloane-Stanley, JA, Ayyub, H, Mann, M, Frayne, J, Garrick, D & Vernimmen, D 2021, 'Identification of the transcription factor MAZ as a regulator of erythropoiesis', Blood advances, vol. 5, no. 15, pp. 3002-3015. https://doi.org/10.1182/bloodadvances.2021004609

APA

Deen, D., Butter, F., Daniels, D. E., Ferrer-Vicens, I., Ferguson, D. C. J., Holland, M. L., Samara, V., Sloane-Stanley, J. A., Ayyub, H., Mann, M., Frayne, J., Garrick, D., & Vernimmen, D. (2021). Identification of the transcription factor MAZ as a regulator of erythropoiesis. Blood advances, 5(15), 3002-3015. https://doi.org/10.1182/bloodadvances.2021004609

Vancouver

Deen D, Butter F, Daniels DE, Ferrer-Vicens I, Ferguson DCJ, Holland ML et al. Identification of the transcription factor MAZ as a regulator of erythropoiesis. Blood advances. 2021 Aug 10;5(15):3002-3015. https://doi.org/10.1182/bloodadvances.2021004609

Author

Deen, Darya ; Butter, Falk ; Daniels, Deborah E ; Ferrer-Vicens, Ivan ; Ferguson, Daniel C J ; Holland, Michelle L ; Samara, Vasiliki ; Sloane-Stanley, Jacqueline A ; Ayyub, Helena ; Mann, Matthias ; Frayne, Jan ; Garrick, David ; Vernimmen, Douglas. / Identification of the transcription factor MAZ as a regulator of erythropoiesis. In: Blood advances. 2021 ; Vol. 5, No. 15. pp. 3002-3015.

Bibtex

@article{78212d0703f2484382840d943d49f23b,
title = "Identification of the transcription factor MAZ as a regulator of erythropoiesis",
abstract = "Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors (TFs). Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as a TF that binds to the promoter of the erythroid-specific human α-globin gene. Genome-wide mapping in primary human erythroid cells revealed that MAZ also occupies active promoters as well as GATA1-bound enhancer elements of key erythroid genes. Consistent with an important role during erythropoiesis, knockdown of MAZ reduces α-globin expression in K562 cells and impairs differentiation in primary human erythroid cells. Genetic variants in the MAZ locus are associated with changes in clinically important human erythroid traits. Taken together, these findings reveal the zinc-finger TF MAZ to be a previously unrecognized regulator of the erythroid differentiation program.",
keywords = "DNA-Binding Proteins/genetics, Erythroid Cells/metabolism, Erythropoiesis/genetics, Gene Expression Regulation, Humans, K562 Cells, Promoter Regions, Genetic, Transcription Factors/genetics",
author = "Darya Deen and Falk Butter and Daniels, {Deborah E} and Ivan Ferrer-Vicens and Ferguson, {Daniel C J} and Holland, {Michelle L} and Vasiliki Samara and Sloane-Stanley, {Jacqueline A} and Helena Ayyub and Matthias Mann and Jan Frayne and David Garrick and Douglas Vernimmen",
note = "{\textcopyright} 2021 by The American Society of Hematology.",
year = "2021",
month = aug,
day = "10",
doi = "10.1182/bloodadvances.2021004609",
language = "English",
volume = "5",
pages = "3002--3015",
journal = "Blood advances",
issn = "2473-9529",
publisher = "American Society of Hematology",
number = "15",

}

RIS

TY - JOUR

T1 - Identification of the transcription factor MAZ as a regulator of erythropoiesis

AU - Deen, Darya

AU - Butter, Falk

AU - Daniels, Deborah E

AU - Ferrer-Vicens, Ivan

AU - Ferguson, Daniel C J

AU - Holland, Michelle L

AU - Samara, Vasiliki

AU - Sloane-Stanley, Jacqueline A

AU - Ayyub, Helena

AU - Mann, Matthias

AU - Frayne, Jan

AU - Garrick, David

AU - Vernimmen, Douglas

N1 - © 2021 by The American Society of Hematology.

PY - 2021/8/10

Y1 - 2021/8/10

N2 - Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors (TFs). Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as a TF that binds to the promoter of the erythroid-specific human α-globin gene. Genome-wide mapping in primary human erythroid cells revealed that MAZ also occupies active promoters as well as GATA1-bound enhancer elements of key erythroid genes. Consistent with an important role during erythropoiesis, knockdown of MAZ reduces α-globin expression in K562 cells and impairs differentiation in primary human erythroid cells. Genetic variants in the MAZ locus are associated with changes in clinically important human erythroid traits. Taken together, these findings reveal the zinc-finger TF MAZ to be a previously unrecognized regulator of the erythroid differentiation program.

AB - Erythropoiesis requires a combination of ubiquitous and tissue-specific transcription factors (TFs). Here, through DNA affinity purification followed by mass spectrometry, we have identified the widely expressed protein MAZ (Myc-associated zinc finger) as a TF that binds to the promoter of the erythroid-specific human α-globin gene. Genome-wide mapping in primary human erythroid cells revealed that MAZ also occupies active promoters as well as GATA1-bound enhancer elements of key erythroid genes. Consistent with an important role during erythropoiesis, knockdown of MAZ reduces α-globin expression in K562 cells and impairs differentiation in primary human erythroid cells. Genetic variants in the MAZ locus are associated with changes in clinically important human erythroid traits. Taken together, these findings reveal the zinc-finger TF MAZ to be a previously unrecognized regulator of the erythroid differentiation program.

KW - DNA-Binding Proteins/genetics

KW - Erythroid Cells/metabolism

KW - Erythropoiesis/genetics

KW - Gene Expression Regulation

KW - Humans

KW - K562 Cells

KW - Promoter Regions, Genetic

KW - Transcription Factors/genetics

U2 - 10.1182/bloodadvances.2021004609

DO - 10.1182/bloodadvances.2021004609

M3 - Journal article

C2 - 34351390

VL - 5

SP - 3002

EP - 3015

JO - Blood advances

JF - Blood advances

SN - 2473-9529

IS - 15

ER -

ID: 276701164