Dynamics in protein translation sustaining T cell preparedness

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Dynamics in protein translation sustaining T cell preparedness. / Wolf, Tobias; Jin, Wenjie; Zoppi, Giada; Vogel, Ian A; Akhmedov, Murodzhon; Bleck, Christopher K E; Beltraminelli, Tim; Rieckmann, Jan C; Ramirez, Neftali J; Benevento, Marco; Notarbartolo, Samuele; Bumann, Dirk; Meissner, Felix; Grimbacher, Bodo; Mann, Matthias; Lanzavecchia, Antonio; Sallusto, Federica; Kwee, Ivo; Geiger, Roger.

In: Nature Immunology, 06.07.2020.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wolf, T, Jin, W, Zoppi, G, Vogel, IA, Akhmedov, M, Bleck, CKE, Beltraminelli, T, Rieckmann, JC, Ramirez, NJ, Benevento, M, Notarbartolo, S, Bumann, D, Meissner, F, Grimbacher, B, Mann, M, Lanzavecchia, A, Sallusto, F, Kwee, I & Geiger, R 2020, 'Dynamics in protein translation sustaining T cell preparedness', Nature Immunology. https://doi.org/10.1038/s41590-020-0714-5

APA

Wolf, T., Jin, W., Zoppi, G., Vogel, I. A., Akhmedov, M., Bleck, C. K. E., Beltraminelli, T., Rieckmann, J. C., Ramirez, N. J., Benevento, M., Notarbartolo, S., Bumann, D., Meissner, F., Grimbacher, B., Mann, M., Lanzavecchia, A., Sallusto, F., Kwee, I., & Geiger, R. (2020). Dynamics in protein translation sustaining T cell preparedness. Nature Immunology. https://doi.org/10.1038/s41590-020-0714-5

Vancouver

Wolf T, Jin W, Zoppi G, Vogel IA, Akhmedov M, Bleck CKE et al. Dynamics in protein translation sustaining T cell preparedness. Nature Immunology. 2020 Jul 6. https://doi.org/10.1038/s41590-020-0714-5

Author

Wolf, Tobias ; Jin, Wenjie ; Zoppi, Giada ; Vogel, Ian A ; Akhmedov, Murodzhon ; Bleck, Christopher K E ; Beltraminelli, Tim ; Rieckmann, Jan C ; Ramirez, Neftali J ; Benevento, Marco ; Notarbartolo, Samuele ; Bumann, Dirk ; Meissner, Felix ; Grimbacher, Bodo ; Mann, Matthias ; Lanzavecchia, Antonio ; Sallusto, Federica ; Kwee, Ivo ; Geiger, Roger. / Dynamics in protein translation sustaining T cell preparedness. In: Nature Immunology. 2020.

Bibtex

@article{1e82922646bd436cb5c98f9b6c23f8d5,
title = "Dynamics in protein translation sustaining T cell preparedness",
abstract = "In response to pathogenic threats, naive T cells rapidly transition from a quiescent to an activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naive and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion following activation. Furthermore, naive T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells (https://www.immunomics.ch).",
author = "Tobias Wolf and Wenjie Jin and Giada Zoppi and Vogel, {Ian A} and Murodzhon Akhmedov and Bleck, {Christopher K E} and Tim Beltraminelli and Rieckmann, {Jan C} and Ramirez, {Neftali J} and Marco Benevento and Samuele Notarbartolo and Dirk Bumann and Felix Meissner and Bodo Grimbacher and Matthias Mann and Antonio Lanzavecchia and Federica Sallusto and Ivo Kwee and Roger Geiger",
year = "2020",
month = jul,
day = "6",
doi = "10.1038/s41590-020-0714-5",
language = "English",
journal = "Nature Immunology",
issn = "1529-2908",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Dynamics in protein translation sustaining T cell preparedness

AU - Wolf, Tobias

AU - Jin, Wenjie

AU - Zoppi, Giada

AU - Vogel, Ian A

AU - Akhmedov, Murodzhon

AU - Bleck, Christopher K E

AU - Beltraminelli, Tim

AU - Rieckmann, Jan C

AU - Ramirez, Neftali J

AU - Benevento, Marco

AU - Notarbartolo, Samuele

AU - Bumann, Dirk

AU - Meissner, Felix

AU - Grimbacher, Bodo

AU - Mann, Matthias

AU - Lanzavecchia, Antonio

AU - Sallusto, Federica

AU - Kwee, Ivo

AU - Geiger, Roger

PY - 2020/7/6

Y1 - 2020/7/6

N2 - In response to pathogenic threats, naive T cells rapidly transition from a quiescent to an activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naive and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion following activation. Furthermore, naive T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells (https://www.immunomics.ch).

AB - In response to pathogenic threats, naive T cells rapidly transition from a quiescent to an activated state, yet the underlying mechanisms are incompletely understood. Using a pulsed SILAC approach, we investigated the dynamics of mRNA translation kinetics and protein turnover in human naive and activated T cells. Our datasets uncovered that transcription factors maintaining T cell quiescence had constitutively high turnover, which facilitated their depletion following activation. Furthermore, naive T cells maintained a surprisingly large number of idling ribosomes as well as 242 repressed mRNA species and a reservoir of glycolytic enzymes. These components were rapidly engaged following stimulation, promoting an immediate translational and glycolytic switch to ramp up the T cell activation program. Our data elucidate new insights into how T cells maintain a prepared state to mount a rapid immune response, and provide a resource of protein turnover, absolute translation kinetics and protein synthesis rates in T cells (https://www.immunomics.ch).

U2 - 10.1038/s41590-020-0714-5

DO - 10.1038/s41590-020-0714-5

M3 - Journal article

C2 - 32632289

JO - Nature Immunology

JF - Nature Immunology

SN - 1529-2908

ER -

ID: 244994465