Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation. / Hoefig, Kai P; Reim, Alexander; Gallus, Christian; Wong, Elaine H; Behrens, Gesine; Conrad, Christine; Xu, Meng; Kifinger, Lisa; Ito-Kureha, Taku; Defourny, Kyra A Y; Geerlof, Arie; Mautner, Josef; Hauck, Stefanie M; Baumjohann, Dirk; Feederle, Regina; Mann, Matthias; Wierer, Michael; Glasmacher, Elke; Heissmeyer, Vigo.

In: Nature Communications, Vol. 12, No. 1, 01.09.2021, p. 5208.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Hoefig, KP, Reim, A, Gallus, C, Wong, EH, Behrens, G, Conrad, C, Xu, M, Kifinger, L, Ito-Kureha, T, Defourny, KAY, Geerlof, A, Mautner, J, Hauck, SM, Baumjohann, D, Feederle, R, Mann, M, Wierer, M, Glasmacher, E & Heissmeyer, V 2021, 'Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation', Nature Communications, vol. 12, no. 1, pp. 5208. https://doi.org/10.1038/s41467-021-25345-5

APA

Hoefig, K. P., Reim, A., Gallus, C., Wong, E. H., Behrens, G., Conrad, C., Xu, M., Kifinger, L., Ito-Kureha, T., Defourny, K. A. Y., Geerlof, A., Mautner, J., Hauck, S. M., Baumjohann, D., Feederle, R., Mann, M., Wierer, M., Glasmacher, E., & Heissmeyer, V. (2021). Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation. Nature Communications, 12(1), 5208. https://doi.org/10.1038/s41467-021-25345-5

Vancouver

Hoefig KP, Reim A, Gallus C, Wong EH, Behrens G, Conrad C et al. Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation. Nature Communications. 2021 Sep 1;12(1):5208. https://doi.org/10.1038/s41467-021-25345-5

Author

Hoefig, Kai P ; Reim, Alexander ; Gallus, Christian ; Wong, Elaine H ; Behrens, Gesine ; Conrad, Christine ; Xu, Meng ; Kifinger, Lisa ; Ito-Kureha, Taku ; Defourny, Kyra A Y ; Geerlof, Arie ; Mautner, Josef ; Hauck, Stefanie M ; Baumjohann, Dirk ; Feederle, Regina ; Mann, Matthias ; Wierer, Michael ; Glasmacher, Elke ; Heissmeyer, Vigo. / Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation. In: Nature Communications. 2021 ; Vol. 12, No. 1. pp. 5208.

Bibtex

@article{3f9e5befb394416e8ebe3ac59fcf5a6b,
title = "Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation",
abstract = "Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This is evident for the Icos mRNA encoding an essential costimulatory receptor that is regulated by several RNA-binding proteins (RBP), including Roquin-1 and Roquin-2. Here, we identify a core RBPome of 798 mouse and 801 human T cell proteins by utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS). The RBPome includes Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we select ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also show Roquin-1/2-dependent and target-specific coregulation of Icos by Celf1 and Igf2bp3. Connecting the cellular RBPome in a post-transcriptional context, we find contributions from multiple RBPs to the prototypic regulation of mRNA targets by individual trans-acting factors.",
keywords = "Animals, DNA-Binding Proteins, Gene Expression Regulation, HEK293 Cells, Humans, Inducible T-Cell Co-Stimulator Protein/genetics, Mice, Proto-Oncogene Proteins c-vav, RNA, Messenger/metabolism, RNA-Binding Proteins/genetics, STAT1 Transcription Factor, STAT4 Transcription Factor, Signal Transduction, T-Lymphocytes, Helper-Inducer/metabolism, Trans-Activators/metabolism, Ubiquitin-Protein Ligases/genetics",
author = "Hoefig, {Kai P} and Alexander Reim and Christian Gallus and Wong, {Elaine H} and Gesine Behrens and Christine Conrad and Meng Xu and Lisa Kifinger and Taku Ito-Kureha and Defourny, {Kyra A Y} and Arie Geerlof and Josef Mautner and Hauck, {Stefanie M} and Dirk Baumjohann and Regina Feederle and Matthias Mann and Michael Wierer and Elke Glasmacher and Vigo Heissmeyer",
note = "{\textcopyright} 2021. The Author(s).",
year = "2021",
month = sep,
day = "1",
doi = "10.1038/s41467-021-25345-5",
language = "English",
volume = "12",
pages = "5208",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - Defining the RBPome of primary T helper cells to elucidate higher-order Roquin-mediated mRNA regulation

AU - Hoefig, Kai P

AU - Reim, Alexander

AU - Gallus, Christian

AU - Wong, Elaine H

AU - Behrens, Gesine

AU - Conrad, Christine

AU - Xu, Meng

AU - Kifinger, Lisa

AU - Ito-Kureha, Taku

AU - Defourny, Kyra A Y

AU - Geerlof, Arie

AU - Mautner, Josef

AU - Hauck, Stefanie M

AU - Baumjohann, Dirk

AU - Feederle, Regina

AU - Mann, Matthias

AU - Wierer, Michael

AU - Glasmacher, Elke

AU - Heissmeyer, Vigo

N1 - © 2021. The Author(s).

PY - 2021/9/1

Y1 - 2021/9/1

N2 - Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This is evident for the Icos mRNA encoding an essential costimulatory receptor that is regulated by several RNA-binding proteins (RBP), including Roquin-1 and Roquin-2. Here, we identify a core RBPome of 798 mouse and 801 human T cell proteins by utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS). The RBPome includes Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we select ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also show Roquin-1/2-dependent and target-specific coregulation of Icos by Celf1 and Igf2bp3. Connecting the cellular RBPome in a post-transcriptional context, we find contributions from multiple RBPs to the prototypic regulation of mRNA targets by individual trans-acting factors.

AB - Post-transcriptional gene regulation in T cells is dynamic and complex as targeted transcripts respond to various factors. This is evident for the Icos mRNA encoding an essential costimulatory receptor that is regulated by several RNA-binding proteins (RBP), including Roquin-1 and Roquin-2. Here, we identify a core RBPome of 798 mouse and 801 human T cell proteins by utilizing global RNA interactome capture (RNA-IC) and orthogonal organic phase separation (OOPS). The RBPome includes Stat1, Stat4 and Vav1 proteins suggesting unexpected functions for these transcription factors and signal transducers. Based on proximity to Roquin-1, we select ~50 RBPs for testing coregulation of Roquin-1/2 targets by induced expression in wild-type or Roquin-1/2-deficient T cells. Besides Roquin-independent contributions from Rbms1 and Cpeb4 we also show Roquin-1/2-dependent and target-specific coregulation of Icos by Celf1 and Igf2bp3. Connecting the cellular RBPome in a post-transcriptional context, we find contributions from multiple RBPs to the prototypic regulation of mRNA targets by individual trans-acting factors.

KW - Animals

KW - DNA-Binding Proteins

KW - Gene Expression Regulation

KW - HEK293 Cells

KW - Humans

KW - Inducible T-Cell Co-Stimulator Protein/genetics

KW - Mice

KW - Proto-Oncogene Proteins c-vav

KW - RNA, Messenger/metabolism

KW - RNA-Binding Proteins/genetics

KW - STAT1 Transcription Factor

KW - STAT4 Transcription Factor

KW - Signal Transduction

KW - T-Lymphocytes, Helper-Inducer/metabolism

KW - Trans-Activators/metabolism

KW - Ubiquitin-Protein Ligases/genetics

U2 - 10.1038/s41467-021-25345-5

DO - 10.1038/s41467-021-25345-5

M3 - Journal article

C2 - 34471108

VL - 12

SP - 5208

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -

ID: 303116308