An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics

Research output: Contribution to journal › Journal article › Research › peer-review

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An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics. / Angelidis, Ilias; Simon, Lukas M; Fernandez, Isis E; Strunz, Maximilian; Mayr, Christoph H; Greiffo, Flavia R; Tsitsiridis, George; Ansari, Meshal; Graf, Elisabeth; Strom, Tim-Matthias; Nagendran, Monica; Desai, Tushar; Eickelberg, Oliver; Mann, Matthias; Theis, Fabian J; Schiller, Herbert B.

In: Nature Communications, Vol. 10, No. 1, 27.02.2019, p. 963.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Angelidis, I, Simon, LM, Fernandez, IE, Strunz, M, Mayr, CH, Greiffo, FR, Tsitsiridis, G, Ansari, M, Graf, E, Strom, T-M, Nagendran, M, Desai, T, Eickelberg, O, Mann, M, Theis, FJ & Schiller, HB 2019, 'An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics', Nature Communications, vol. 10, no. 1, pp. 963. https://doi.org/10.1038/s41467-019-08831-9

APA

Angelidis, I., Simon, L. M., Fernandez, I. E., Strunz, M., Mayr, C. H., Greiffo, F. R., Tsitsiridis, G., Ansari, M., Graf, E., Strom, T-M., Nagendran, M., Desai, T., Eickelberg, O., Mann, M., Theis, F. J., & Schiller, H. B. (2019). An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics. Nature Communications, 10(1), 963. https://doi.org/10.1038/s41467-019-08831-9

Vancouver

Angelidis I, Simon LM, Fernandez IE, Strunz M, Mayr CH, Greiffo FR et al. An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics. Nature Communications. 2019 Feb 27;10(1):963. https://doi.org/10.1038/s41467-019-08831-9

Author

Angelidis, Ilias ; Simon, Lukas M ; Fernandez, Isis E ; Strunz, Maximilian ; Mayr, Christoph H ; Greiffo, Flavia R ; Tsitsiridis, George ; Ansari, Meshal ; Graf, Elisabeth ; Strom, Tim-Matthias ; Nagendran, Monica ; Desai, Tushar ; Eickelberg, Oliver ; Mann, Matthias ; Theis, Fabian J ; Schiller, Herbert B. / An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics. In: Nature Communications. 2019 ; Vol. 10, No. 1. pp. 963.

Bibtex

@article{89d5b1f1230e4fd29ddb98cff590ad3f,
title = "An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics",
abstract = "Aging promotes lung function decline and susceptibility to chronic lung diseases, which are the third leading cause of death worldwide. Here, we use single cell transcriptomics and mass spectrometry-based proteomics to quantify changes in cellular activity states across 30 cell types and chart the lung proteome of young and old mice. We show that aging leads to increased transcriptional noise, indicating deregulated epigenetic control. We observe cell type-specific effects of aging, uncovering increased cholesterol biosynthesis in type-2 pneumocytes and lipofibroblasts and altered relative frequency of airway epithelial cells as hallmarks of lung aging. Proteomic profiling reveals extracellular matrix remodeling in old mice, including increased collagen IV and XVI and decreased Fraser syndrome complex proteins and collagen XIV. Computational integration of the aging proteome with the single cell transcriptomes predicts the cellular source of regulated proteins and creates an unbiased reference map of the aging lung.",
author = "Ilias Angelidis and Simon, {Lukas M} and Fernandez, {Isis E} and Maximilian Strunz and Mayr, {Christoph H} and Greiffo, {Flavia R} and George Tsitsiridis and Meshal Ansari and Elisabeth Graf and Tim-Matthias Strom and Monica Nagendran and Tushar Desai and Oliver Eickelberg and Matthias Mann and Theis, {Fabian J} and Schiller, {Herbert B}",
year = "2019",
month = feb,
day = "27",
doi = "10.1038/s41467-019-08831-9",
language = "English",
volume = "10",
pages = "963",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",
number = "1",

}

RIS

TY - JOUR

T1 - An atlas of the aging lung mapped by single cell transcriptomics and deep tissue proteomics

AU - Angelidis, Ilias

AU - Simon, Lukas M

AU - Fernandez, Isis E

AU - Strunz, Maximilian

AU - Mayr, Christoph H

AU - Greiffo, Flavia R

AU - Tsitsiridis, George

AU - Ansari, Meshal

AU - Graf, Elisabeth

AU - Strom, Tim-Matthias

AU - Nagendran, Monica

AU - Desai, Tushar

AU - Eickelberg, Oliver

AU - Mann, Matthias

AU - Theis, Fabian J

AU - Schiller, Herbert B

PY - 2019/2/27

Y1 - 2019/2/27

N2 - Aging promotes lung function decline and susceptibility to chronic lung diseases, which are the third leading cause of death worldwide. Here, we use single cell transcriptomics and mass spectrometry-based proteomics to quantify changes in cellular activity states across 30 cell types and chart the lung proteome of young and old mice. We show that aging leads to increased transcriptional noise, indicating deregulated epigenetic control. We observe cell type-specific effects of aging, uncovering increased cholesterol biosynthesis in type-2 pneumocytes and lipofibroblasts and altered relative frequency of airway epithelial cells as hallmarks of lung aging. Proteomic profiling reveals extracellular matrix remodeling in old mice, including increased collagen IV and XVI and decreased Fraser syndrome complex proteins and collagen XIV. Computational integration of the aging proteome with the single cell transcriptomes predicts the cellular source of regulated proteins and creates an unbiased reference map of the aging lung.

AB - Aging promotes lung function decline and susceptibility to chronic lung diseases, which are the third leading cause of death worldwide. Here, we use single cell transcriptomics and mass spectrometry-based proteomics to quantify changes in cellular activity states across 30 cell types and chart the lung proteome of young and old mice. We show that aging leads to increased transcriptional noise, indicating deregulated epigenetic control. We observe cell type-specific effects of aging, uncovering increased cholesterol biosynthesis in type-2 pneumocytes and lipofibroblasts and altered relative frequency of airway epithelial cells as hallmarks of lung aging. Proteomic profiling reveals extracellular matrix remodeling in old mice, including increased collagen IV and XVI and decreased Fraser syndrome complex proteins and collagen XIV. Computational integration of the aging proteome with the single cell transcriptomes predicts the cellular source of regulated proteins and creates an unbiased reference map of the aging lung.

U2 - 10.1038/s41467-019-08831-9

DO - 10.1038/s41467-019-08831-9

M3 - Journal article

C2 - 30814501

VL - 10

SP - 963

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

IS - 1

ER -

ID: 214464264