Recycling of modified H2A-H2B provides short-term memory of chromatin states

Research output: Contribution to journalJournal articleResearchpeer-review


  • Fulltext

    Final published version, 5.09 MB, PDF document

Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The histone H3-H4 modification landscape is restored by parental histone recycling and modification of new histones. How DNA replication impacts on histone H2A-H2B is currently unknown. Here, we measure H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub1, H2BK120ub1, and H2A.Z are recycled accurately during DNA replication. Modified H2A-H2B are segregated symmetrically to daughter strands via POLA1 on the lagging strand, but independent of H3-H4 recycling. Post-replication, H2A-H2B modification and variant landscapes are quickly restored, and H2AK119ub1 guides accurate restoration of H3K27me3. This work reveals epigenetic transmission of parental H2A-H2B during DNA replication and identifies cross talk between H3-H4 and H2A-H2B modifications in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates restoration of stable H3-H4 chromatin states.

Original languageEnglish
Issue number5
Pages (from-to)1050-1065.e19
Number of pages35
Publication statusPublished - 2023

Bibliographical note

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Number of downloads are based on statistics from Google Scholar and

No data available

ID: 337355397