Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis

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Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis. / Kuzdere, Tahsin; Flury, Valentin; Schalch, Thomas; Iesmantavicius, Vytautas; Hess, Daniel; Bühler, Marc.

In: EMBO Reports, Vol. 24, No. 1, e55928, 2023.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kuzdere, T, Flury, V, Schalch, T, Iesmantavicius, V, Hess, D & Bühler, M 2023, 'Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis', EMBO Reports, vol. 24, no. 1, e55928. https://doi.org/10.15252/embr.202255928

APA

Kuzdere, T., Flury, V., Schalch, T., Iesmantavicius, V., Hess, D., & Bühler, M. (2023). Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis. EMBO Reports, 24(1), [e55928]. https://doi.org/10.15252/embr.202255928

Vancouver

Kuzdere T, Flury V, Schalch T, Iesmantavicius V, Hess D, Bühler M. Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis. EMBO Reports. 2023;24(1). e55928. https://doi.org/10.15252/embr.202255928

Author

Kuzdere, Tahsin ; Flury, Valentin ; Schalch, Thomas ; Iesmantavicius, Vytautas ; Hess, Daniel ; Bühler, Marc. / Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis. In: EMBO Reports. 2023 ; Vol. 24, No. 1.

Bibtex

@article{9d74120892b04d30b5baae238e5c9970,
title = "Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis",
abstract = "Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis.",
keywords = "chromosome segregation, fission yeast, histone methylation, meiosis, phosphorylation",
author = "Tahsin Kuzdere and Valentin Flury and Thomas Schalch and Vytautas Iesmantavicius and Daniel Hess and Marc B{\"u}hler",
note = "Publisher Copyright: {\textcopyright} 2022 Friedrich Miescher Institute for Biomedical Research. Published under the terms of the CC BY 4.0 license.",
year = "2023",
doi = "10.15252/embr.202255928",
language = "English",
volume = "24",
journal = "E M B O Reports",
issn = "1469-221X",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Differential phosphorylation of Clr4SUV39H by Cdk1 accompanies a histone H3 methylation switch that is essential for gametogenesis

AU - Kuzdere, Tahsin

AU - Flury, Valentin

AU - Schalch, Thomas

AU - Iesmantavicius, Vytautas

AU - Hess, Daniel

AU - Bühler, Marc

N1 - Publisher Copyright: © 2022 Friedrich Miescher Institute for Biomedical Research. Published under the terms of the CC BY 4.0 license.

PY - 2023

Y1 - 2023

N2 - Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis.

AB - Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis.

KW - chromosome segregation

KW - fission yeast

KW - histone methylation

KW - meiosis

KW - phosphorylation

U2 - 10.15252/embr.202255928

DO - 10.15252/embr.202255928

M3 - Journal article

C2 - 36408846

AN - SCOPUS:85142260411

VL - 24

JO - E M B O Reports

JF - E M B O Reports

SN - 1469-221X

IS - 1

M1 - e55928

ER -

ID: 337385559