An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing

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An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing. / Shimada, Yukiko; Carl, Sarah H.; Skribbe, Merle; Flury, Valentin; Kuzdere, Tahsin; Kempf, Georg; Bühler, Marc.

In: PLOS Genetics, Vol. 17, No. 6, e1009645, 2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Shimada, Y, Carl, SH, Skribbe, M, Flury, V, Kuzdere, T, Kempf, G & Bühler, M 2021, 'An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing', PLOS Genetics, vol. 17, no. 6, e1009645. https://doi.org/10.1371/journal.pgen.1009645

APA

Shimada, Y., Carl, S. H., Skribbe, M., Flury, V., Kuzdere, T., Kempf, G., & Bühler, M. (2021). An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing. PLOS Genetics, 17(6), [e1009645]. https://doi.org/10.1371/journal.pgen.1009645

Vancouver

Shimada Y, Carl SH, Skribbe M, Flury V, Kuzdere T, Kempf G et al. An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing. PLOS Genetics. 2021;17(6). e1009645. https://doi.org/10.1371/journal.pgen.1009645

Author

Shimada, Yukiko ; Carl, Sarah H. ; Skribbe, Merle ; Flury, Valentin ; Kuzdere, Tahsin ; Kempf, Georg ; Bühler, Marc. / An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing. In: PLOS Genetics. 2021 ; Vol. 17, No. 6.

Bibtex

@article{05acc332225a429398646f4048318f28,
title = "An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing",
abstract = "Small non-protein coding RNAs are involved in pathways that control the genome at the level of chromatin. In Schizosaccharomyces pombe, small interfering RNAs (siRNAs) are required for the faithful propagation of heterochromatin that is found at peri-centromeric repeats. In contrast to repetitive DNA, protein-coding genes are refractory to siRNA-mediated heterochromatin formation, unless siRNAs are expressed in mutant cells. Here we report the identification of 20 novel mutant alleles that enable de novo formation of heterochromatin at a euchromatic protein-coding gene by using trans-acting siRNAs as triggers. For example, a single amino acid substitution in the pre-mRNA cleavage factor Yth1 enables siRNAs to trigger silent chromatin formation with unparalleled efficiency. Our results are consistent with a kinetic nascent transcript processing model for the inhibition of small-RNA-directed de novo formation of heterochromatin and lay a foundation for further mechanistic dissection of cellular activities that counteract epigenetic gene silencing.",
author = "Yukiko Shimada and Carl, {Sarah H.} and Merle Skribbe and Valentin Flury and Tahsin Kuzdere and Georg Kempf and Marc B{\"u}hler",
note = "Publisher Copyright: Copyright: {\textcopyright} 2021 Shimada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",
year = "2021",
doi = "10.1371/journal.pgen.1009645",
language = "English",
volume = "17",
journal = "P L o S Genetics",
issn = "1553-7390",
publisher = "Public Library of Science",
number = "6",

}

RIS

TY - JOUR

T1 - An enhancer screen identifies new suppressors of small-RNA-mediated epigenetic gene silencing

AU - Shimada, Yukiko

AU - Carl, Sarah H.

AU - Skribbe, Merle

AU - Flury, Valentin

AU - Kuzdere, Tahsin

AU - Kempf, Georg

AU - Bühler, Marc

N1 - Publisher Copyright: Copyright: © 2021 Shimada et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2021

Y1 - 2021

N2 - Small non-protein coding RNAs are involved in pathways that control the genome at the level of chromatin. In Schizosaccharomyces pombe, small interfering RNAs (siRNAs) are required for the faithful propagation of heterochromatin that is found at peri-centromeric repeats. In contrast to repetitive DNA, protein-coding genes are refractory to siRNA-mediated heterochromatin formation, unless siRNAs are expressed in mutant cells. Here we report the identification of 20 novel mutant alleles that enable de novo formation of heterochromatin at a euchromatic protein-coding gene by using trans-acting siRNAs as triggers. For example, a single amino acid substitution in the pre-mRNA cleavage factor Yth1 enables siRNAs to trigger silent chromatin formation with unparalleled efficiency. Our results are consistent with a kinetic nascent transcript processing model for the inhibition of small-RNA-directed de novo formation of heterochromatin and lay a foundation for further mechanistic dissection of cellular activities that counteract epigenetic gene silencing.

AB - Small non-protein coding RNAs are involved in pathways that control the genome at the level of chromatin. In Schizosaccharomyces pombe, small interfering RNAs (siRNAs) are required for the faithful propagation of heterochromatin that is found at peri-centromeric repeats. In contrast to repetitive DNA, protein-coding genes are refractory to siRNA-mediated heterochromatin formation, unless siRNAs are expressed in mutant cells. Here we report the identification of 20 novel mutant alleles that enable de novo formation of heterochromatin at a euchromatic protein-coding gene by using trans-acting siRNAs as triggers. For example, a single amino acid substitution in the pre-mRNA cleavage factor Yth1 enables siRNAs to trigger silent chromatin formation with unparalleled efficiency. Our results are consistent with a kinetic nascent transcript processing model for the inhibition of small-RNA-directed de novo formation of heterochromatin and lay a foundation for further mechanistic dissection of cellular activities that counteract epigenetic gene silencing.

U2 - 10.1371/journal.pgen.1009645

DO - 10.1371/journal.pgen.1009645

M3 - Journal article

C2 - 34157021

AN - SCOPUS:85108916703

VL - 17

JO - P L o S Genetics

JF - P L o S Genetics

SN - 1553-7390

IS - 6

M1 - e1009645

ER -

ID: 337387880