Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation

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Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation. / Edupuganti, Raghu Ram; Harikumar, Arigela; Aaronson, Yair; Biran, Alva; Sailaja, Badi Sri; Nissim-Rafinia, Malka; Azad, Gajendra Kumar; Cohen, Malkiel A.; Park, Jung Eun; Shivalila, Chikdu S.; Markoulaki, Styliani; Sze, Siu Kwan; Jaenisch, Rudolf; Meshorer, Eran.

In: Stem Cell Reports, Vol. 9, No. 4, 2017, p. 1291-1303.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Edupuganti, RR, Harikumar, A, Aaronson, Y, Biran, A, Sailaja, BS, Nissim-Rafinia, M, Azad, GK, Cohen, MA, Park, JE, Shivalila, CS, Markoulaki, S, Sze, SK, Jaenisch, R & Meshorer, E 2017, 'Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation', Stem Cell Reports, vol. 9, no. 4, pp. 1291-1303. https://doi.org/10.1016/j.stemcr.2017.08.021

APA

Edupuganti, R. R., Harikumar, A., Aaronson, Y., Biran, A., Sailaja, B. S., Nissim-Rafinia, M., Azad, G. K., Cohen, M. A., Park, J. E., Shivalila, C. S., Markoulaki, S., Sze, S. K., Jaenisch, R., & Meshorer, E. (2017). Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation. Stem Cell Reports, 9(4), 1291-1303. https://doi.org/10.1016/j.stemcr.2017.08.021

Vancouver

Edupuganti RR, Harikumar A, Aaronson Y, Biran A, Sailaja BS, Nissim-Rafinia M et al. Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation. Stem Cell Reports. 2017;9(4):1291-1303. https://doi.org/10.1016/j.stemcr.2017.08.021

Author

Edupuganti, Raghu Ram ; Harikumar, Arigela ; Aaronson, Yair ; Biran, Alva ; Sailaja, Badi Sri ; Nissim-Rafinia, Malka ; Azad, Gajendra Kumar ; Cohen, Malkiel A. ; Park, Jung Eun ; Shivalila, Chikdu S. ; Markoulaki, Styliani ; Sze, Siu Kwan ; Jaenisch, Rudolf ; Meshorer, Eran. / Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation. In: Stem Cell Reports. 2017 ; Vol. 9, No. 4. pp. 1291-1303.

Bibtex

@article{4a73adc7190a488887a4b131b63ba4e1,
title = "Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation",
abstract = "Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage. In this article, Meshorer and colleagues screen the endogenously tagged fluorescent library they generated (see the accompanying paper by Harikumar et al.) and identify a linker histone chaperone, SET/TAF-I, to be involved in regulating mouse ESC pluripotency and early differentiation decisions. They show that SET has two isoforms regulated by alternative promoters and pluripotency factors, which are switched during early differentiation. SETα/SETβ replacement is important for ESC differentiation.",
keywords = "chromatin, differentiation, embryonic stem cells, epigenetics, histone chaperone, histone dynamics, pluripotency, SET, TAF-I, TAFI",
author = "Edupuganti, {Raghu Ram} and Arigela Harikumar and Yair Aaronson and Alva Biran and Sailaja, {Badi Sri} and Malka Nissim-Rafinia and Azad, {Gajendra Kumar} and Cohen, {Malkiel A.} and Park, {Jung Eun} and Shivalila, {Chikdu S.} and Styliani Markoulaki and Sze, {Siu Kwan} and Rudolf Jaenisch and Eran Meshorer",
note = "Publisher Copyright: {\textcopyright} 2017 The Authors",
year = "2017",
doi = "10.1016/j.stemcr.2017.08.021",
language = "English",
volume = "9",
pages = "1291--1303",
journal = "Stem Cell Reports",
issn = "2213-6711",
publisher = "Cell Press",
number = "4",

}

RIS

TY - JOUR

T1 - Alternative SET/TAFI Promoters Regulate Embryonic Stem Cell Differentiation

AU - Edupuganti, Raghu Ram

AU - Harikumar, Arigela

AU - Aaronson, Yair

AU - Biran, Alva

AU - Sailaja, Badi Sri

AU - Nissim-Rafinia, Malka

AU - Azad, Gajendra Kumar

AU - Cohen, Malkiel A.

AU - Park, Jung Eun

AU - Shivalila, Chikdu S.

AU - Markoulaki, Styliani

AU - Sze, Siu Kwan

AU - Jaenisch, Rudolf

AU - Meshorer, Eran

N1 - Publisher Copyright: © 2017 The Authors

PY - 2017

Y1 - 2017

N2 - Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage. In this article, Meshorer and colleagues screen the endogenously tagged fluorescent library they generated (see the accompanying paper by Harikumar et al.) and identify a linker histone chaperone, SET/TAF-I, to be involved in regulating mouse ESC pluripotency and early differentiation decisions. They show that SET has two isoforms regulated by alternative promoters and pluripotency factors, which are switched during early differentiation. SETα/SETβ replacement is important for ESC differentiation.

AB - Embryonic stem cells (ESCs) are regulated by pluripotency-related transcription factors in concert with chromatin regulators. To identify additional stem cell regulators, we screened a library of endogenously labeled fluorescent fusion proteins in mouse ESCs for fluorescence loss during differentiation. We identified SET, which displayed a rapid isoform shift during early differentiation from the predominant isoform in ESCs, SETα, to the primary isoform in differentiated cells, SETβ, through alternative promoters. SETα is selectively bound and regulated by pluripotency factors. SET depletion causes proliferation slowdown and perturbed neuronal differentiation in vitro and developmental arrest in vivo, and photobleaching methods demonstrate SET's role in maintaining a dynamic chromatin state in ESCs. This work identifies an important regulator of pluripotency and early differentiation, which is controlled by alternative promoter usage. In this article, Meshorer and colleagues screen the endogenously tagged fluorescent library they generated (see the accompanying paper by Harikumar et al.) and identify a linker histone chaperone, SET/TAF-I, to be involved in regulating mouse ESC pluripotency and early differentiation decisions. They show that SET has two isoforms regulated by alternative promoters and pluripotency factors, which are switched during early differentiation. SETα/SETβ replacement is important for ESC differentiation.

KW - chromatin

KW - differentiation

KW - embryonic stem cells

KW - epigenetics

KW - histone chaperone

KW - histone dynamics

KW - pluripotency

KW - SET

KW - TAF-I

KW - TAFI

U2 - 10.1016/j.stemcr.2017.08.021

DO - 10.1016/j.stemcr.2017.08.021

M3 - Journal article

C2 - 28966118

AN - SCOPUS:85030696288

VL - 9

SP - 1291

EP - 1303

JO - Stem Cell Reports

JF - Stem Cell Reports

SN - 2213-6711

IS - 4

ER -

ID: 380217010