Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts

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Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts. / Larsen, Nicolai B.; Gao, Alan O.; Sparks, Justin L; Gallina, Irene; Wu, R Alex; Mann, Matthias; Räschle, Markus; Walter, Johannes C; Duxin, Julien P.

In: Molecular Cell, Vol. 73, No. 3, 2019, p. 574-588.e7.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Larsen, NB, Gao, AO, Sparks, JL, Gallina, I, Wu, RA, Mann, M, Räschle, M, Walter, JC & Duxin, JP 2019, 'Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts', Molecular Cell, vol. 73, no. 3, pp. 574-588.e7. https://doi.org/10.1016/j.molcel.2018.11.024

APA

Larsen, N. B., Gao, A. O., Sparks, J. L., Gallina, I., Wu, R. A., Mann, M., Räschle, M., Walter, J. C., & Duxin, J. P. (2019). Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts. Molecular Cell, 73(3), 574-588.e7. https://doi.org/10.1016/j.molcel.2018.11.024

Vancouver

Larsen NB, Gao AO, Sparks JL, Gallina I, Wu RA, Mann M et al. Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts. Molecular Cell. 2019;73(3):574-588.e7. https://doi.org/10.1016/j.molcel.2018.11.024

Author

Larsen, Nicolai B. ; Gao, Alan O. ; Sparks, Justin L ; Gallina, Irene ; Wu, R Alex ; Mann, Matthias ; Räschle, Markus ; Walter, Johannes C ; Duxin, Julien P. / Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts. In: Molecular Cell. 2019 ; Vol. 73, No. 3. pp. 574-588.e7.

Bibtex

@article{9cff4358cc554efabf47e208d9a72c40,
title = "Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts",
abstract = "DNA-protein crosslinks (DPCs) are bulky lesions that interfere with DNA metabolism and therefore threaten genomic integrity. Recent studies implicate the metalloprotease SPRTN in S phase removal of DPCs, but how SPRTN is targeted to DPCs during DNA replication is unknown. Using Xenopus egg extracts that recapitulate replication-coupled DPC proteolysis, we show that DPCs can be degraded by SPRTN or the proteasome, which act as independent DPC proteases. Proteasome recruitment requires DPC polyubiquitylation, which is partially dependent on the ubiquitin ligase activity of TRAIP. In contrast, SPRTN-mediated DPC degradation does not require DPC polyubiquitylation but instead depends on nascent strand extension to within a few nucleotides of the lesion, implying that polymerase stalling at the DPC activates SPRTN on both leading and lagging strand templates. Our results demonstrate that SPRTN and proteasome activities are coupled to DNA replication by distinct mechanisms that promote replication across immovable protein barriers.",
author = "Larsen, {Nicolai B.} and Gao, {Alan O.} and Sparks, {Justin L} and Irene Gallina and Wu, {R Alex} and Matthias Mann and Markus R{\"a}schle and Walter, {Johannes C} and Duxin, {Julien P.}",
year = "2019",
doi = "10.1016/j.molcel.2018.11.024",
language = "English",
volume = "73",
pages = "574--588.e7",
journal = "Molecular Cell",
issn = "1097-2765",
publisher = "Cell Press",
number = "3",

}

RIS

TY - JOUR

T1 - Replication-Coupled DNA-Protein Crosslink Repair by SPRTN and the Proteasome in Xenopus Egg Extracts

AU - Larsen, Nicolai B.

AU - Gao, Alan O.

AU - Sparks, Justin L

AU - Gallina, Irene

AU - Wu, R Alex

AU - Mann, Matthias

AU - Räschle, Markus

AU - Walter, Johannes C

AU - Duxin, Julien P.

PY - 2019

Y1 - 2019

N2 - DNA-protein crosslinks (DPCs) are bulky lesions that interfere with DNA metabolism and therefore threaten genomic integrity. Recent studies implicate the metalloprotease SPRTN in S phase removal of DPCs, but how SPRTN is targeted to DPCs during DNA replication is unknown. Using Xenopus egg extracts that recapitulate replication-coupled DPC proteolysis, we show that DPCs can be degraded by SPRTN or the proteasome, which act as independent DPC proteases. Proteasome recruitment requires DPC polyubiquitylation, which is partially dependent on the ubiquitin ligase activity of TRAIP. In contrast, SPRTN-mediated DPC degradation does not require DPC polyubiquitylation but instead depends on nascent strand extension to within a few nucleotides of the lesion, implying that polymerase stalling at the DPC activates SPRTN on both leading and lagging strand templates. Our results demonstrate that SPRTN and proteasome activities are coupled to DNA replication by distinct mechanisms that promote replication across immovable protein barriers.

AB - DNA-protein crosslinks (DPCs) are bulky lesions that interfere with DNA metabolism and therefore threaten genomic integrity. Recent studies implicate the metalloprotease SPRTN in S phase removal of DPCs, but how SPRTN is targeted to DPCs during DNA replication is unknown. Using Xenopus egg extracts that recapitulate replication-coupled DPC proteolysis, we show that DPCs can be degraded by SPRTN or the proteasome, which act as independent DPC proteases. Proteasome recruitment requires DPC polyubiquitylation, which is partially dependent on the ubiquitin ligase activity of TRAIP. In contrast, SPRTN-mediated DPC degradation does not require DPC polyubiquitylation but instead depends on nascent strand extension to within a few nucleotides of the lesion, implying that polymerase stalling at the DPC activates SPRTN on both leading and lagging strand templates. Our results demonstrate that SPRTN and proteasome activities are coupled to DNA replication by distinct mechanisms that promote replication across immovable protein barriers.

U2 - 10.1016/j.molcel.2018.11.024

DO - 10.1016/j.molcel.2018.11.024

M3 - Journal article

C2 - 30595436

VL - 73

SP - 574-588.e7

JO - Molecular Cell

JF - Molecular Cell

SN - 1097-2765

IS - 3

ER -

ID: 211995096