OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres. / Kristensen, David M; Nielsen, John E; Kalisz, Mark; Dalgaard, Marlene D; Audouze, Karine; Larsen, Malene E; Jacobsen, Grete K; Horn, Thomas; Brunak, Søren; Skakkebaek, Niels E; Leffers, Henrik; Kristensen, David Møbjerg; Nielsen, John E; Kalisz, Mark; Dalgaard, Marlene D; Audouze, Karine Marie Laure; Larsen, Malene E; Jacobsen, Grete K; Horn, Thomas; Brunak, Søren; Skakkebæk, Niels Erik; Leffers, Henrik.

In: Molecular Human Reproduction, Vol. 16, No. 11, 01.11.2010, p. 835-45.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Kristensen, DM, Nielsen, JE, Kalisz, M, Dalgaard, MD, Audouze, K, Larsen, ME, Jacobsen, GK, Horn, T, Brunak, S, Skakkebaek, NE, Leffers, H, Kristensen, DM, Nielsen, JE, Kalisz, M, Dalgaard, MD, Audouze, KML, Larsen, ME, Jacobsen, GK, Horn, T, Brunak, S, Skakkebæk, NE & Leffers, H 2010, 'OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres', Molecular Human Reproduction, vol. 16, no. 11, pp. 835-45. https://doi.org/10.1093/molehr/gaq008, https://doi.org/10.1093/molehr/gaq008

APA

Kristensen, D. M., Nielsen, J. E., Kalisz, M., Dalgaard, M. D., Audouze, K., Larsen, M. E., Jacobsen, G. K., Horn, T., Brunak, S., Skakkebaek, N. E., Leffers, H., Kristensen, D. M., Nielsen, J. E., Kalisz, M., Dalgaard, M. D., Audouze, K. M. L., Larsen, M. E., Jacobsen, G. K., Horn, T., ... Leffers, H. (2010). OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres. Molecular Human Reproduction, 16(11), 835-45. https://doi.org/10.1093/molehr/gaq008, https://doi.org/10.1093/molehr/gaq008

Vancouver

Kristensen DM, Nielsen JE, Kalisz M, Dalgaard MD, Audouze K, Larsen ME et al. OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres. Molecular Human Reproduction. 2010 Nov 1;16(11):835-45. https://doi.org/10.1093/molehr/gaq008, https://doi.org/10.1093/molehr/gaq008

Author

Kristensen, David M ; Nielsen, John E ; Kalisz, Mark ; Dalgaard, Marlene D ; Audouze, Karine ; Larsen, Malene E ; Jacobsen, Grete K ; Horn, Thomas ; Brunak, Søren ; Skakkebaek, Niels E ; Leffers, Henrik ; Kristensen, David Møbjerg ; Nielsen, John E ; Kalisz, Mark ; Dalgaard, Marlene D ; Audouze, Karine Marie Laure ; Larsen, Malene E ; Jacobsen, Grete K ; Horn, Thomas ; Brunak, Søren ; Skakkebæk, Niels Erik ; Leffers, Henrik. / OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres. In: Molecular Human Reproduction. 2010 ; Vol. 16, No. 11. pp. 835-45.

Bibtex

@article{f1fd2860957011df928f000ea68e967b,
title = "OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres",
abstract = "The transcription factor OCT4 plays a crucial role in the earliest differentiation of the mammalian embryo and in self-renewal of embryonic stem cells. However, it remains controversial whether this gene is also expressed in somatic tissues. Here we use a combination of RT-PCR on whole and microdissected tissues, in situ hybridisation, immunohistochemistry, and Western blotting to show that OCT4 and SOX2 together with downstream targets, UTF1 and REX1, are expressed in the human male urogenital tract. We further supported these results by analysis of DNA methylation of a region in the OCT4 promoter. In culture, human primary epididymis cells formed spheres that continued to express the investigated genes for at least 20 days. Transcriptomic analysis of cultured cells showed up-regulation of CD29, CD44, and CD133 that are normally associated with sphere-forming cancer stem cells. Furthermore, stimulation with retinoic acid resulted in down-regulation of OCT4 expression, however, without multilineage differentiation. Our results show that OCT4 and associated genes are expressed in somatic epithelial cells from the urogenital tract and that these cells can form spheres, a general marker of stem cell behaviour.",
author = "Kristensen, {David M} and Nielsen, {John E} and Mark Kalisz and Dalgaard, {Marlene D} and Karine Audouze and Larsen, {Malene E} and Jacobsen, {Grete K} and Thomas Horn and S{\o}ren Brunak and Skakkebaek, {Niels E} and Henrik Leffers and Kristensen, {David M{\o}bjerg} and Nielsen, {John E} and Mark Kalisz and Dalgaard, {Marlene D} and Audouze, {Karine Marie Laure} and Larsen, {Malene E} and Jacobsen, {Grete K} and Thomas Horn and S{\o}ren Brunak and Skakkeb{\ae}k, {Niels Erik} and Henrik Leffers",
year = "2010",
month = nov,
day = "1",
doi = "10.1093/molehr/gaq008",
language = "English",
volume = "16",
pages = "835--45",
journal = "Molecular Human Reproduction",
issn = "1360-9947",
publisher = "Oxford University Press",
number = "11",

}

RIS

TY - JOUR

T1 - OCT4 and downstream factors are expressed in human somatic urogenital epithelia and in culture of epididymal spheres

AU - Kristensen, David M

AU - Nielsen, John E

AU - Kalisz, Mark

AU - Dalgaard, Marlene D

AU - Audouze, Karine

AU - Larsen, Malene E

AU - Jacobsen, Grete K

AU - Horn, Thomas

AU - Brunak, Søren

AU - Skakkebaek, Niels E

AU - Leffers, Henrik

AU - Kristensen, David Møbjerg

AU - Nielsen, John E

AU - Kalisz, Mark

AU - Dalgaard, Marlene D

AU - Audouze, Karine Marie Laure

AU - Larsen, Malene E

AU - Jacobsen, Grete K

AU - Horn, Thomas

AU - Brunak, Søren

AU - Skakkebæk, Niels Erik

AU - Leffers, Henrik

PY - 2010/11/1

Y1 - 2010/11/1

N2 - The transcription factor OCT4 plays a crucial role in the earliest differentiation of the mammalian embryo and in self-renewal of embryonic stem cells. However, it remains controversial whether this gene is also expressed in somatic tissues. Here we use a combination of RT-PCR on whole and microdissected tissues, in situ hybridisation, immunohistochemistry, and Western blotting to show that OCT4 and SOX2 together with downstream targets, UTF1 and REX1, are expressed in the human male urogenital tract. We further supported these results by analysis of DNA methylation of a region in the OCT4 promoter. In culture, human primary epididymis cells formed spheres that continued to express the investigated genes for at least 20 days. Transcriptomic analysis of cultured cells showed up-regulation of CD29, CD44, and CD133 that are normally associated with sphere-forming cancer stem cells. Furthermore, stimulation with retinoic acid resulted in down-regulation of OCT4 expression, however, without multilineage differentiation. Our results show that OCT4 and associated genes are expressed in somatic epithelial cells from the urogenital tract and that these cells can form spheres, a general marker of stem cell behaviour.

AB - The transcription factor OCT4 plays a crucial role in the earliest differentiation of the mammalian embryo and in self-renewal of embryonic stem cells. However, it remains controversial whether this gene is also expressed in somatic tissues. Here we use a combination of RT-PCR on whole and microdissected tissues, in situ hybridisation, immunohistochemistry, and Western blotting to show that OCT4 and SOX2 together with downstream targets, UTF1 and REX1, are expressed in the human male urogenital tract. We further supported these results by analysis of DNA methylation of a region in the OCT4 promoter. In culture, human primary epididymis cells formed spheres that continued to express the investigated genes for at least 20 days. Transcriptomic analysis of cultured cells showed up-regulation of CD29, CD44, and CD133 that are normally associated with sphere-forming cancer stem cells. Furthermore, stimulation with retinoic acid resulted in down-regulation of OCT4 expression, however, without multilineage differentiation. Our results show that OCT4 and associated genes are expressed in somatic epithelial cells from the urogenital tract and that these cells can form spheres, a general marker of stem cell behaviour.

U2 - 10.1093/molehr/gaq008

DO - 10.1093/molehr/gaq008

M3 - Journal article

C2 - 20123703

VL - 16

SP - 835

EP - 845

JO - Molecular Human Reproduction

JF - Molecular Human Reproduction

SN - 1360-9947

IS - 11

ER -

ID: 20970294