Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology. / Rojas, Ana M; Santamaria, Anna; Malik, Rainer; Jensen, Thomas Skøt; Körner, Roman; Morilla, Ian; de Juan, David; Krallinger, Martin; Hansen, Daniel Aaen; Hoffmann, Robert; Lees, Jonathan; Reid, Adam; Yeats, Corin; Wehner, Anja; Elowe, Sabine; Clegg, Andrew B; Brunak, Søren; Nigg, Erich A; Orengo, Christine; Valencia, Alfonso; Ranea, Juan A G.

In: P L o S One, Vol. 7, No. 3, 2012, p. e31813.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Rojas, AM, Santamaria, A, Malik, R, Jensen, TS, Körner, R, Morilla, I, de Juan, D, Krallinger, M, Hansen, DA, Hoffmann, R, Lees, J, Reid, A, Yeats, C, Wehner, A, Elowe, S, Clegg, AB, Brunak, S, Nigg, EA, Orengo, C, Valencia, A & Ranea, JAG 2012, 'Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology', P L o S One, vol. 7, no. 3, pp. e31813. https://doi.org/10.1371/journal.pone.0031813

APA

Rojas, A. M., Santamaria, A., Malik, R., Jensen, T. S., Körner, R., Morilla, I., de Juan, D., Krallinger, M., Hansen, D. A., Hoffmann, R., Lees, J., Reid, A., Yeats, C., Wehner, A., Elowe, S., Clegg, A. B., Brunak, S., Nigg, E. A., Orengo, C., ... Ranea, J. A. G. (2012). Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology. P L o S One, 7(3), e31813. https://doi.org/10.1371/journal.pone.0031813

Vancouver

Rojas AM, Santamaria A, Malik R, Jensen TS, Körner R, Morilla I et al. Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology. P L o S One. 2012;7(3):e31813. https://doi.org/10.1371/journal.pone.0031813

Author

Rojas, Ana M ; Santamaria, Anna ; Malik, Rainer ; Jensen, Thomas Skøt ; Körner, Roman ; Morilla, Ian ; de Juan, David ; Krallinger, Martin ; Hansen, Daniel Aaen ; Hoffmann, Robert ; Lees, Jonathan ; Reid, Adam ; Yeats, Corin ; Wehner, Anja ; Elowe, Sabine ; Clegg, Andrew B ; Brunak, Søren ; Nigg, Erich A ; Orengo, Christine ; Valencia, Alfonso ; Ranea, Juan A G. / Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology. In: P L o S One. 2012 ; Vol. 7, No. 3. pp. e31813.

Bibtex

@article{a9228f6073154e28b1c3d6c3a0e7d788,
title = "Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology",
abstract = "The mitotic spindle is an essential molecular machine involved in cell division, whose composition has been studied extensively by detailed cellular biology, high-throughput proteomics, and RNA interference experiments. However, because of its dynamic organization and complex regulation it is difficult to obtain a complete description of its molecular composition. We have implemented an integrated computational approach to characterize novel human spindle components and have analysed in detail the individual candidates predicted to be spindle proteins, as well as the network of predicted relations connecting known and putative spindle proteins. The subsequent experimental validation of a number of predicted novel proteins confirmed not only their association with the spindle apparatus but also their role in mitosis. We found that 75% of our tested proteins are localizing to the spindle apparatus compared to a success rate of 35% when expert knowledge alone was used. We compare our results to the previously published MitoCheck study and see that our approach does validate some findings by this consortium. Further, we predict so-called {"}hidden spindle hub{"}, proteins whose network of interactions is still poorly characterised by experimental means and which are thought to influence the functionality of the mitotic spindle on a large scale. Our analyses suggest that we are still far from knowing the complete repertoire of functionally important components of the human spindle network. Combining integrated bio-computational approaches and single gene experimental follow-ups could be key to exploring the still hidden regions of the human spindle system.",
author = "Rojas, {Ana M} and Anna Santamaria and Rainer Malik and Jensen, {Thomas Sk{\o}t} and Roman K{\"o}rner and Ian Morilla and {de Juan}, David and Martin Krallinger and Hansen, {Daniel Aaen} and Robert Hoffmann and Jonathan Lees and Adam Reid and Corin Yeats and Anja Wehner and Sabine Elowe and Clegg, {Andrew B} and S{\o}ren Brunak and Nigg, {Erich A} and Christine Orengo and Alfonso Valencia and Ranea, {Juan A G}",
year = "2012",
doi = "10.1371/journal.pone.0031813",
language = "English",
volume = "7",
pages = "e31813",
journal = "PLoS ONE",
issn = "1932-6203",
publisher = "Public Library of Science",
number = "3",

}

RIS

TY - JOUR

T1 - Uncovering the molecular machinery of the human spindle--an integration of wet and dry systems biology

AU - Rojas, Ana M

AU - Santamaria, Anna

AU - Malik, Rainer

AU - Jensen, Thomas Skøt

AU - Körner, Roman

AU - Morilla, Ian

AU - de Juan, David

AU - Krallinger, Martin

AU - Hansen, Daniel Aaen

AU - Hoffmann, Robert

AU - Lees, Jonathan

AU - Reid, Adam

AU - Yeats, Corin

AU - Wehner, Anja

AU - Elowe, Sabine

AU - Clegg, Andrew B

AU - Brunak, Søren

AU - Nigg, Erich A

AU - Orengo, Christine

AU - Valencia, Alfonso

AU - Ranea, Juan A G

PY - 2012

Y1 - 2012

N2 - The mitotic spindle is an essential molecular machine involved in cell division, whose composition has been studied extensively by detailed cellular biology, high-throughput proteomics, and RNA interference experiments. However, because of its dynamic organization and complex regulation it is difficult to obtain a complete description of its molecular composition. We have implemented an integrated computational approach to characterize novel human spindle components and have analysed in detail the individual candidates predicted to be spindle proteins, as well as the network of predicted relations connecting known and putative spindle proteins. The subsequent experimental validation of a number of predicted novel proteins confirmed not only their association with the spindle apparatus but also their role in mitosis. We found that 75% of our tested proteins are localizing to the spindle apparatus compared to a success rate of 35% when expert knowledge alone was used. We compare our results to the previously published MitoCheck study and see that our approach does validate some findings by this consortium. Further, we predict so-called "hidden spindle hub", proteins whose network of interactions is still poorly characterised by experimental means and which are thought to influence the functionality of the mitotic spindle on a large scale. Our analyses suggest that we are still far from knowing the complete repertoire of functionally important components of the human spindle network. Combining integrated bio-computational approaches and single gene experimental follow-ups could be key to exploring the still hidden regions of the human spindle system.

AB - The mitotic spindle is an essential molecular machine involved in cell division, whose composition has been studied extensively by detailed cellular biology, high-throughput proteomics, and RNA interference experiments. However, because of its dynamic organization and complex regulation it is difficult to obtain a complete description of its molecular composition. We have implemented an integrated computational approach to characterize novel human spindle components and have analysed in detail the individual candidates predicted to be spindle proteins, as well as the network of predicted relations connecting known and putative spindle proteins. The subsequent experimental validation of a number of predicted novel proteins confirmed not only their association with the spindle apparatus but also their role in mitosis. We found that 75% of our tested proteins are localizing to the spindle apparatus compared to a success rate of 35% when expert knowledge alone was used. We compare our results to the previously published MitoCheck study and see that our approach does validate some findings by this consortium. Further, we predict so-called "hidden spindle hub", proteins whose network of interactions is still poorly characterised by experimental means and which are thought to influence the functionality of the mitotic spindle on a large scale. Our analyses suggest that we are still far from knowing the complete repertoire of functionally important components of the human spindle network. Combining integrated bio-computational approaches and single gene experimental follow-ups could be key to exploring the still hidden regions of the human spindle system.

U2 - 10.1371/journal.pone.0031813

DO - 10.1371/journal.pone.0031813

M3 - Journal article

C2 - 22427808

VL - 7

SP - e31813

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 3

ER -

ID: 40804019