Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation

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Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation. / Zhang, Gang; Mendez, Blanca Lopez; Sedgwick, Garry G; Nilsson, Jakob.

In: Nature Communications, Vol. 7, 12256, 2016, p. 1-12.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, G, Mendez, BL, Sedgwick, GG & Nilsson, J 2016, 'Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation', Nature Communications, vol. 7, 12256, pp. 1-12. https://doi.org/10.1038/ncomms12256

APA

Zhang, G., Mendez, B. L., Sedgwick, G. G., & Nilsson, J. (2016). Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation. Nature Communications, 7, 1-12. [12256]. https://doi.org/10.1038/ncomms12256

Vancouver

Zhang G, Mendez BL, Sedgwick GG, Nilsson J. Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation. Nature Communications. 2016;7:1-12. 12256. https://doi.org/10.1038/ncomms12256

Author

Zhang, Gang ; Mendez, Blanca Lopez ; Sedgwick, Garry G ; Nilsson, Jakob. / Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation. In: Nature Communications. 2016 ; Vol. 7. pp. 1-12.

Bibtex

@article{9119030aaacb4616a698e44d676efe42,
title = "Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation",
abstract = "The BubR1/Bub3 complex is an important regulator of chromosome segregation as it facilitates proper kinetochore-microtubule interactions and is also an essential component of the spindle assembly checkpoint (SAC). Whether BubR1/Bub3 localization to kinetochores in human cells stimulates SAC signalling or only contributes to kinetochore-microtubule interactions is debated. Here we show that two distinct pools of BubR1/Bub3 exist at kinetochores and we uncouple these with defined BubR1/Bub3 mutants to address their function. The major kinetochore pool of BubR1/Bub3 is dependent on direct Bub1/Bub3 binding and is required for chromosome alignment but not for the SAC. A distinct pool of BubR1/Bub3 localizes by directly binding to phosphorylated MELT repeats on the outer kinetochore protein KNL1. When we prevent the direct binding of BubR1/Bub3 to KNL1 the checkpoint is weakened because BubR1/Bub3 is not incorporated into checkpoint complexes efficiently. In conclusion, kinetochore localization supports both known functions of BubR1/Bub3.",
keywords = "Journal Article",
author = "Gang Zhang and Mendez, {Blanca Lopez} and Sedgwick, {Garry G} and Jakob Nilsson",
year = "2016",
doi = "10.1038/ncomms12256",
language = "English",
volume = "7",
pages = "1--12",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "nature publishing group",

}

RIS

TY - JOUR

T1 - Two functionally distinct kinetochore pools of BubR1 ensure accurate chromosome segregation

AU - Zhang, Gang

AU - Mendez, Blanca Lopez

AU - Sedgwick, Garry G

AU - Nilsson, Jakob

PY - 2016

Y1 - 2016

N2 - The BubR1/Bub3 complex is an important regulator of chromosome segregation as it facilitates proper kinetochore-microtubule interactions and is also an essential component of the spindle assembly checkpoint (SAC). Whether BubR1/Bub3 localization to kinetochores in human cells stimulates SAC signalling or only contributes to kinetochore-microtubule interactions is debated. Here we show that two distinct pools of BubR1/Bub3 exist at kinetochores and we uncouple these with defined BubR1/Bub3 mutants to address their function. The major kinetochore pool of BubR1/Bub3 is dependent on direct Bub1/Bub3 binding and is required for chromosome alignment but not for the SAC. A distinct pool of BubR1/Bub3 localizes by directly binding to phosphorylated MELT repeats on the outer kinetochore protein KNL1. When we prevent the direct binding of BubR1/Bub3 to KNL1 the checkpoint is weakened because BubR1/Bub3 is not incorporated into checkpoint complexes efficiently. In conclusion, kinetochore localization supports both known functions of BubR1/Bub3.

AB - The BubR1/Bub3 complex is an important regulator of chromosome segregation as it facilitates proper kinetochore-microtubule interactions and is also an essential component of the spindle assembly checkpoint (SAC). Whether BubR1/Bub3 localization to kinetochores in human cells stimulates SAC signalling or only contributes to kinetochore-microtubule interactions is debated. Here we show that two distinct pools of BubR1/Bub3 exist at kinetochores and we uncouple these with defined BubR1/Bub3 mutants to address their function. The major kinetochore pool of BubR1/Bub3 is dependent on direct Bub1/Bub3 binding and is required for chromosome alignment but not for the SAC. A distinct pool of BubR1/Bub3 localizes by directly binding to phosphorylated MELT repeats on the outer kinetochore protein KNL1. When we prevent the direct binding of BubR1/Bub3 to KNL1 the checkpoint is weakened because BubR1/Bub3 is not incorporated into checkpoint complexes efficiently. In conclusion, kinetochore localization supports both known functions of BubR1/Bub3.

KW - Journal Article

U2 - 10.1038/ncomms12256

DO - 10.1038/ncomms12256

M3 - Journal article

C2 - 27457023

VL - 7

SP - 1

EP - 12

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 12256

ER -

ID: 164181889