Transcriptional control of mycobacterial DNA damage response by sigma adaptation

Research output: Contribution to journal › Journal article › Research › peer-review

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Transcriptional control of mycobacterial DNA damage response by sigma adaptation. / Müller, Andreas U; Kummer, Eva; Schilling, Charlotte M; Ban, Nenad; Weber-Ban, Eilika.

In: Science Advances, Vol. 7, No. 49, eabl4064, 03.12.2021.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Müller, AU, Kummer, E, Schilling, CM, Ban, N & Weber-Ban, E 2021, 'Transcriptional control of mycobacterial DNA damage response by sigma adaptation', Science Advances, vol. 7, no. 49, eabl4064. https://doi.org/10.1126/sciadv.abl4064

APA

Müller, A. U., Kummer, E., Schilling, C. M., Ban, N., & Weber-Ban, E. (2021). Transcriptional control of mycobacterial DNA damage response by sigma adaptation. Science Advances, 7(49), [eabl4064]. https://doi.org/10.1126/sciadv.abl4064

Vancouver

Müller AU, Kummer E, Schilling CM, Ban N, Weber-Ban E. Transcriptional control of mycobacterial DNA damage response by sigma adaptation. Science Advances. 2021 Dec 3;7(49). eabl4064. https://doi.org/10.1126/sciadv.abl4064

Author

Müller, Andreas U ; Kummer, Eva ; Schilling, Charlotte M ; Ban, Nenad ; Weber-Ban, Eilika. / Transcriptional control of mycobacterial DNA damage response by sigma adaptation. In: Science Advances. 2021 ; Vol. 7, No. 49.

Bibtex

@article{08b1c5b71daf48df8bceed4b816004e1,
title = "Transcriptional control of mycobacterial DNA damage response by sigma adaptation",
abstract = "Transcriptional activator PafBC is the key regulator of the mycobacterial DNA damage response and controls around 150 genes, including genes involved in the canonical SOS response, through an unknown molecular mechanism. Using a combination of biochemistry and cryo–electron microscopy, we demonstrate that PafBC in the presence of single-stranded DNA activates transcription by reprogramming the canonical −10 and −35 promoter specificity of RNA polymerase associated with the housekeeping sigma subunit. We determine the structure of this transcription initiation complex, revealing a unique mode of promoter recognition, which we term “sigma adaptation.” PafBC inserts between DNA and sigma factor to mediate recognition of hybrid promoters lacking the −35 but featuring the canonical −10 and a PafBC-specific −26 element. Sigma adaptation may constitute a more general mechanism of transcriptional control in mycobacteria.",
author = "M{\"u}ller, {Andreas U} and Eva Kummer and Schilling, {Charlotte M} and Nenad Ban and Eilika Weber-Ban",
year = "2021",
month = dec,
day = "3",
doi = "10.1126/sciadv.abl4064",
language = "English",
volume = "7",
journal = "Science advances",
issn = "2375-2548",
publisher = "American Association for the Advancement of Science",
number = "49",

}

RIS

TY - JOUR

T1 - Transcriptional control of mycobacterial DNA damage response by sigma adaptation

AU - Müller, Andreas U

AU - Kummer, Eva

AU - Schilling, Charlotte M

AU - Ban, Nenad

AU - Weber-Ban, Eilika

PY - 2021/12/3

Y1 - 2021/12/3

N2 - Transcriptional activator PafBC is the key regulator of the mycobacterial DNA damage response and controls around 150 genes, including genes involved in the canonical SOS response, through an unknown molecular mechanism. Using a combination of biochemistry and cryo–electron microscopy, we demonstrate that PafBC in the presence of single-stranded DNA activates transcription by reprogramming the canonical −10 and −35 promoter specificity of RNA polymerase associated with the housekeeping sigma subunit. We determine the structure of this transcription initiation complex, revealing a unique mode of promoter recognition, which we term “sigma adaptation.” PafBC inserts between DNA and sigma factor to mediate recognition of hybrid promoters lacking the −35 but featuring the canonical −10 and a PafBC-specific −26 element. Sigma adaptation may constitute a more general mechanism of transcriptional control in mycobacteria.

AB - Transcriptional activator PafBC is the key regulator of the mycobacterial DNA damage response and controls around 150 genes, including genes involved in the canonical SOS response, through an unknown molecular mechanism. Using a combination of biochemistry and cryo–electron microscopy, we demonstrate that PafBC in the presence of single-stranded DNA activates transcription by reprogramming the canonical −10 and −35 promoter specificity of RNA polymerase associated with the housekeeping sigma subunit. We determine the structure of this transcription initiation complex, revealing a unique mode of promoter recognition, which we term “sigma adaptation.” PafBC inserts between DNA and sigma factor to mediate recognition of hybrid promoters lacking the −35 but featuring the canonical −10 and a PafBC-specific −26 element. Sigma adaptation may constitute a more general mechanism of transcriptional control in mycobacteria.

U2 - 10.1126/sciadv.abl4064

DO - 10.1126/sciadv.abl4064

M3 - Journal article

C2 - 34851662

VL - 7

JO - Science advances

JF - Science advances

SN - 2375-2548

IS - 49

M1 - eabl4064

ER -

ID: 303112310