TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells. / Pedersen, Rune Troelsgaard; Kruse, Thomas; Nilsson, Jakob; Østergaard, Vibe Hallundbæk; Lisby, Michael.

In: Journal of Cell Biology, Vol. 210, No. 4, 2015, p. 565-582.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Pedersen, RT, Kruse, T, Nilsson, J, Østergaard, VH & Lisby, M 2015, 'TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells', Journal of Cell Biology, vol. 210, no. 4, pp. 565-582. https://doi.org/10.1083/jcb.201502107

APA

Pedersen, R. T., Kruse, T., Nilsson, J., Østergaard, V. H., & Lisby, M. (2015). TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells. Journal of Cell Biology, 210(4), 565-582. https://doi.org/10.1083/jcb.201502107

Vancouver

Pedersen RT, Kruse T, Nilsson J, Østergaard VH, Lisby M. TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells. Journal of Cell Biology. 2015;210(4):565-582. https://doi.org/10.1083/jcb.201502107

Author

Pedersen, Rune Troelsgaard ; Kruse, Thomas ; Nilsson, Jakob ; Østergaard, Vibe Hallundbæk ; Lisby, Michael. / TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells. In: Journal of Cell Biology. 2015 ; Vol. 210, No. 4. pp. 565-582.

Bibtex

@article{cf85a83fffcc49c1b099a9233e1ed938,
title = "TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells",
abstract = "Genome integrity is critically dependent on timely DNA replication and accurate chromosome segregation. Replication stress delays replication into G2/M, which in turn impairs proper chromosome segregation and inflicts DNA damage on the daughter cells. Here we show that TopBP1 forms foci upon mitotic entry. In early mitosis, TopBP1 marks sites of and promotes unscheduled DNA synthesis. Moreover, TopBP1 is required for focus formation of the structure-selective nuclease and scaffold protein SLX4 in mitosis. Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise temporal depletion of TopBP1 just before mitotic entry induced formation of 53BP1 NBs in the next cell cycle, showing that TopBP1 acts to reduce transmission of DNA damage to G1 daughter cells. Based on these results, we propose that TopBP1 maintains genome integrity in mitosis by controlling chromatin recruitment of SLX4 and by facilitating unscheduled DNA synthesis.",
author = "Pedersen, {Rune Troelsgaard} and Thomas Kruse and Jakob Nilsson and {\O}stergaard, {Vibe Hallundb{\ae}k} and Michael Lisby",
note = "{\textcopyright} 2015 Pedersen et al.",
year = "2015",
doi = "10.1083/jcb.201502107",
language = "English",
volume = "210",
pages = "565--582",
journal = "Journal of Cell Biology",
issn = "0021-9525",
publisher = "Rockefeller University Press",
number = "4",

}

RIS

TY - JOUR

T1 - TopBP1 is required at mitosis to reduce transmission of DNA damage to G1 daughter cells

AU - Pedersen, Rune Troelsgaard

AU - Kruse, Thomas

AU - Nilsson, Jakob

AU - Østergaard, Vibe Hallundbæk

AU - Lisby, Michael

N1 - © 2015 Pedersen et al.

PY - 2015

Y1 - 2015

N2 - Genome integrity is critically dependent on timely DNA replication and accurate chromosome segregation. Replication stress delays replication into G2/M, which in turn impairs proper chromosome segregation and inflicts DNA damage on the daughter cells. Here we show that TopBP1 forms foci upon mitotic entry. In early mitosis, TopBP1 marks sites of and promotes unscheduled DNA synthesis. Moreover, TopBP1 is required for focus formation of the structure-selective nuclease and scaffold protein SLX4 in mitosis. Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise temporal depletion of TopBP1 just before mitotic entry induced formation of 53BP1 NBs in the next cell cycle, showing that TopBP1 acts to reduce transmission of DNA damage to G1 daughter cells. Based on these results, we propose that TopBP1 maintains genome integrity in mitosis by controlling chromatin recruitment of SLX4 and by facilitating unscheduled DNA synthesis.

AB - Genome integrity is critically dependent on timely DNA replication and accurate chromosome segregation. Replication stress delays replication into G2/M, which in turn impairs proper chromosome segregation and inflicts DNA damage on the daughter cells. Here we show that TopBP1 forms foci upon mitotic entry. In early mitosis, TopBP1 marks sites of and promotes unscheduled DNA synthesis. Moreover, TopBP1 is required for focus formation of the structure-selective nuclease and scaffold protein SLX4 in mitosis. Persistent TopBP1 foci transition into 53BP1 nuclear bodies (NBs) in G1 and precise temporal depletion of TopBP1 just before mitotic entry induced formation of 53BP1 NBs in the next cell cycle, showing that TopBP1 acts to reduce transmission of DNA damage to G1 daughter cells. Based on these results, we propose that TopBP1 maintains genome integrity in mitosis by controlling chromatin recruitment of SLX4 and by facilitating unscheduled DNA synthesis.

U2 - 10.1083/jcb.201502107

DO - 10.1083/jcb.201502107

M3 - Journal article

C2 - 26283799

VL - 210

SP - 565

EP - 582

JO - Journal of Cell Biology

JF - Journal of Cell Biology

SN - 0021-9525

IS - 4

ER -

ID: 144285969