TY - JOUR

T1 - The urokinase receptor homolog Haldisin is a novel differentiation marker of stratum granulosum in squamous epithelia

AU - Gårdsvoll, Henrik

AU - Kriegbaum, Mette C

AU - Hertz, Emil P

AU - Alpízar-Alpízar, Warner

AU - Ploug, Michael

PY - 2013/11

Y1 - 2013/11

N2 - Several members of the Ly-6/uPAR (LU)-protein domain family are differentially expressed in human squamous epithelia. In some cases, they even play important roles in maintaining skin homeostasis, as exemplified by the secreted single domain member, SLURP-1, the deficiency of which is associated with the development of palmoplantar hyperkeratosis in the congenital skin disorder Mal de Meleda. In the present study, we have characterized a new member of the LU-protein domain family, which we find to be predominantly expressed in the stratum granulosum of human skin, thus resembling the expression of SLURP-1. In accordance with its expression pattern, we denote this protein product, which is encoded by the LYPD5 gene, as Haldisin (human antigen with LU-domains expressed in skin). Two of the five human glycolipid-anchored membrane proteins with multiple LU-domains characterized so far are predominantly confined to squamous epithelia (i.e., C4.4A), to stratum spinosum, and Haldisin to stratum granulosum under normal homeostatic conditions. Whether Haldisin is a prognostic biomarker for certain epithelial malignancies, like C4.4A and SLURP-1, remains to be explored.

AB - Several members of the Ly-6/uPAR (LU)-protein domain family are differentially expressed in human squamous epithelia. In some cases, they even play important roles in maintaining skin homeostasis, as exemplified by the secreted single domain member, SLURP-1, the deficiency of which is associated with the development of palmoplantar hyperkeratosis in the congenital skin disorder Mal de Meleda. In the present study, we have characterized a new member of the LU-protein domain family, which we find to be predominantly expressed in the stratum granulosum of human skin, thus resembling the expression of SLURP-1. In accordance with its expression pattern, we denote this protein product, which is encoded by the LYPD5 gene, as Haldisin (human antigen with LU-domains expressed in skin). Two of the five human glycolipid-anchored membrane proteins with multiple LU-domains characterized so far are predominantly confined to squamous epithelia (i.e., C4.4A), to stratum spinosum, and Haldisin to stratum granulosum under normal homeostatic conditions. Whether Haldisin is a prognostic biomarker for certain epithelial malignancies, like C4.4A and SLURP-1, remains to be explored.

KW - Animals

KW - Cloning, Molecular

KW - Drosophila

KW - Epidermis

KW - Fluorescent Antibody Technique

KW - Gene Expression

KW - Gene Expression Regulation, Neoplastic

KW - HEK293 Cells

KW - Humans

KW - Immunohistochemistry

KW - Mice

KW - Neoplasms, Squamous Cell

KW - Rats

KW - Rats, Sprague-Dawley

KW - Receptors, Urokinase Plasminogen Activator

KW - Recombinant Proteins

U2 - 10.1369/0022155413501879

DO - 10.1369/0022155413501879

M3 - Journal article

C2 - 23896969

VL - 61

SP - 802

EP - 813

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 11

ER -