The transcriptionally active regions in the genome of Bacillus subtilis

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The majority of all genes have so far been identified and annotated systematically through in silico gene finding. Here we report the finding of 3662 strand-specific transcriptionally active regions (TARs) in the genome of Bacillus subtilis by the use of tiling arrays. We have measured the genome-wide expression during mid-exponential growth on rich (LB) and minimal (M9) medium. The identified TARs account for 77.3% of the genes as they are currently annotated and additionally we find 84 putative non-coding RNAs (ncRNAs) and 127 antisense transcripts. One ncRNA, ncr22, is predicted to act as a translational control on cstA and an antisense transcript was observed opposite the housekeeping sigma factor sigA. Through this work we have discovered a long conserved 3' untranslated region (UTR) in a group of membrane-associated genes that is predicted to fold into a large and highly stable secondary structure. One of the genes having this tail is efeN, which encodes a target of the twin-arginine translocase (Tat) protein translocation system.

Original languageEnglish
JournalMolecular Microbiology
Issue number6
Pages (from-to)1043-57
Number of pages15
Publication statusPublished - Sep 2009
Externally publishedYes

    Research areas

  • Bacillus subtilis/genetics, Base Sequence, Culture Media/chemistry, Gene Expression Profiling/methods, Models, Molecular, Molecular Sequence Data, Nucleic Acid Conformation, RNA, Antisense/biosynthesis, RNA, Untranslated/biosynthesis, Transcription, Genetic

ID: 210773333