The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores. / Zhang, Gang; Kelstrup, Christian D; Hu, Xiao-Wen; Hansen, Mathilde J Kaas; Singleton, Martin R; Olsen, Jesper V; Nilsson, Jakob.

In: Journal of Cell Science, 2012, p. 3243-3253.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Zhang, G, Kelstrup, CD, Hu, X-W, Hansen, MJK, Singleton, MR, Olsen, JV & Nilsson, J 2012, 'The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores', Journal of Cell Science, pp. 3243-3253. https://doi.org/10.1242/jcs.104208

APA

Zhang, G., Kelstrup, C. D., Hu, X-W., Hansen, M. J. K., Singleton, M. R., Olsen, J. V., & Nilsson, J. (2012). The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores. Journal of Cell Science, 3243-3253. https://doi.org/10.1242/jcs.104208

Vancouver

Zhang G, Kelstrup CD, Hu X-W, Hansen MJK, Singleton MR, Olsen JV et al. The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores. Journal of Cell Science. 2012;3243-3253. https://doi.org/10.1242/jcs.104208

Author

Zhang, Gang ; Kelstrup, Christian D ; Hu, Xiao-Wen ; Hansen, Mathilde J Kaas ; Singleton, Martin R ; Olsen, Jesper V ; Nilsson, Jakob. / The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores. In: Journal of Cell Science. 2012 ; pp. 3243-3253.

Bibtex

@article{c11da94359e246ab8f04c68762e52d5b,
title = "The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores",
abstract = "The Ndc80 complex establishes end-on attachment of kinetochores to microtubules essential for chromosome segregation. The Ndc80 subunit is characterized by an N-terminal region, that binds directly to microtubules, and a long coiled-coil region that interacts with Nuf2. A loop region in Ndc80 that generates a kink in the structure disrupts the long coiled-coil region but the exact function of this loop is not clear.Here we show that this loop region is essential for end-on attachment of kinetochores to microtubules in human cells. Cells expressing loop mutants of Ndc80 are unable to align the chromosomes and stable kinetochore fibers are absent. Through quantitative mass spectrometry and immunofluorescence we find that the binding of the Ska complex depends on the loop region explaining why end-on attachment is defective. This underscores the importance of the Ndc80 loop region in coordinating chromosome segregation through the recruitment of specific proteins to the kinetochore.",
author = "Gang Zhang and Kelstrup, {Christian D} and Xiao-Wen Hu and Hansen, {Mathilde J Kaas} and Singleton, {Martin R} and Olsen, {Jesper V} and Jakob Nilsson",
year = "2012",
doi = "10.1242/jcs.104208",
language = "English",
pages = "3243--3253",
journal = "Journal of Cell Science",
issn = "0021-9533",
publisher = "The/Company of Biologists Ltd.",

}

RIS

TY - JOUR

T1 - The Ndc80 internal loop is required for recruitment of the Ska complex to establish end-on microtubule attachment to kinetochores

AU - Zhang, Gang

AU - Kelstrup, Christian D

AU - Hu, Xiao-Wen

AU - Hansen, Mathilde J Kaas

AU - Singleton, Martin R

AU - Olsen, Jesper V

AU - Nilsson, Jakob

PY - 2012

Y1 - 2012

N2 - The Ndc80 complex establishes end-on attachment of kinetochores to microtubules essential for chromosome segregation. The Ndc80 subunit is characterized by an N-terminal region, that binds directly to microtubules, and a long coiled-coil region that interacts with Nuf2. A loop region in Ndc80 that generates a kink in the structure disrupts the long coiled-coil region but the exact function of this loop is not clear.Here we show that this loop region is essential for end-on attachment of kinetochores to microtubules in human cells. Cells expressing loop mutants of Ndc80 are unable to align the chromosomes and stable kinetochore fibers are absent. Through quantitative mass spectrometry and immunofluorescence we find that the binding of the Ska complex depends on the loop region explaining why end-on attachment is defective. This underscores the importance of the Ndc80 loop region in coordinating chromosome segregation through the recruitment of specific proteins to the kinetochore.

AB - The Ndc80 complex establishes end-on attachment of kinetochores to microtubules essential for chromosome segregation. The Ndc80 subunit is characterized by an N-terminal region, that binds directly to microtubules, and a long coiled-coil region that interacts with Nuf2. A loop region in Ndc80 that generates a kink in the structure disrupts the long coiled-coil region but the exact function of this loop is not clear.Here we show that this loop region is essential for end-on attachment of kinetochores to microtubules in human cells. Cells expressing loop mutants of Ndc80 are unable to align the chromosomes and stable kinetochore fibers are absent. Through quantitative mass spectrometry and immunofluorescence we find that the binding of the Ska complex depends on the loop region explaining why end-on attachment is defective. This underscores the importance of the Ndc80 loop region in coordinating chromosome segregation through the recruitment of specific proteins to the kinetochore.

U2 - 10.1242/jcs.104208

DO - 10.1242/jcs.104208

M3 - Journal article

C2 - 22454517

SP - 3243

EP - 3253

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

ER -

ID: 40291402